BACKGROUND: The two-layer organ preservation method (TLM) based on oxygenated perfluorocarbon overlaid with University of Wisconsin (UW) solution has been successfully used in clinical islet and experimental heart and intestine transplantation. We tested whether this technique would prevent tissue damage and improve kidney function in a model of syngeneic kidney transplantation with prolonged ischemia time. METHODS: Kidneys were stored for 24 h either in UW solution (n = 16), with TLM (n = 16) or transplanted immediately (control group, n = 12). In half of the animals, survival was observed and in the other animals grafts were procured for semiquantitative histological scoring and TUNEL apoptosis assessment 24 h after transplantation. RESULTS: One-month survival rates in the UW, TLM and control groups were 12.5, 62.5 and 100%, respectively (UW vs. TLM, p < 0.01). Median creatinine levels 24 h after transplantation were 381, 299 and 121 microM, respectively (UW vs. TLM, p < 0.02). Histological scoring showed more severe tissue damage in the UW group than in the TLM group (p < 0.05). Apoptosis was more frequent in the UW group than in the TLM group (p < 0.05). CONCLUSION: We demonstrated for the first time that conservation with TLM significantly improves the outcome of kidney transplantation in a rat model and should therefore be further studied in larger animals.
- MeSH
- adenosin MeSH
- alopurinol MeSH
- apoptóza MeSH
- časové faktory MeSH
- fluorokarbony MeSH
- glutathion MeSH
- inzulin MeSH
- kreatinin krev MeSH
- krevní náhražky MeSH
- krysa rodu rattus MeSH
- ledviny zranění patologie patofyziologie MeSH
- potkani inbrední BN MeSH
- přežívání štěpu MeSH
- rafinosa MeSH
- reperfuzní poškození patologie prevence a kontrola MeSH
- roztoky pro uchovávání orgánů MeSH
- studená ischemie metody MeSH
- transplantace izogenní MeSH
- transplantace ledvin metody patologie fyziologie MeSH
- uchovávání orgánů metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
