Tularemie patří mezi celosvětově rozšířené zoonózy a i přes její relativně nízkou incidenci v našich podmínkách by neměla být v rámci diferenciální diagnostiky opomíjena. V popisované kazuistice bychom chtěli upozornit na klíště jako možný vektor přenosu ulceroglandulární formy tularemie u dětí a současně vyzvednout výhody metody PCR, která může být použita jako časná a spolehlivá metoda detekce obtížně kultivovatelné bakterie Francisella tularensis.

Tularaemia is a widespread zoonosis, which should be considered in differential diagnostics of lymphadenopathy despite of relatively low incidence of the disease. In the presented case we would like to point out a tick bite as a possible way of transmission of an ulceroglandular form of tularaemia in children as well as underline PCR method as an early and reliable form of detection.

• MeSH
• Anti-Bacterial Agents MeSH
• Ciprofloxacin administration & dosage   therapeutic use   MeSH
• Francisella tularensis pathogenicity   MeSH
• Humans MeSH
• Lymphadenopathy surgery   etiology   MeSH
• Tick-Borne Diseases diagnosis   drug therapy   pathology   MeSH
• Face abnormalities   surgery   pathology   MeSH
• Child, Preschool MeSH
• Tularemia * diagnosis   drug therapy   pathology   MeSH
• Zoonoses diagnosis   drug therapy   pathology   MeSH
• Check Tag
• Humans MeSH
• Child, Preschool MeSH
• Female MeSH
• Publication type
• Case Reports MeSH

Background: The proportion of intensive care unit (ICU) admissions in children that have and have not been directly caused by SARS-CoV-2 remains unclear. The aim of the study is to analyse a cohort of children admitted to the ICU with SARS-CoV-2 and determine whether the infection was the primary cause of their hospitalisation, a significant contributor, a suspected accomplice, or an incidental finding. Methods: This was a retrospective observational study of all the children admitted to the ICU with SARS-CoV-2 from March 2020 to February 2022 from the South Moravia region. The aim of the study was to assess whether the hospitalisation was likely to be directly caused by the virus (i.e., patients with acute COVID-19; the COVID group), whether the virus was a significant contributor to the hospitalisation (i.e., patients with multisystem inflammatory syndrome in children due to COVID-19; the MIS-C group), whether it may have contributed to the worsening of their underlying disease (the WORSENING group), or whether it was an incidental finding very likely unrelated to hospitalisation where SARS-CoV-2 positivity merely placed patients in the COVID-19 unit (the ISOLATION group). The groups were compared using a series of secondary outcomes. Results: The study population represented 150 paediatric ICU cases (age 8.6; IQR 3.5−13.3 years), with 66.7% being male. The COVID group represented 32.7% of cases (49/150); MIS-C, 30% (45/150); WORSENING, 14.7% (22/150); and ISOLATION, 22.7% (34/150). The median length of hospitalisation was found for the MIS-C group (11 days; 9 days in the ICU), the COVID group (6 days; five days in the ICU), WORSENING group (4.5 days; 4.5 days in the ICU) and the ISOLATION group (5.5 days; 3.5 days in the ICU), where the difference was significant (p < 0.001). Asymptomatic and mild cases were most common in the WORSENING (36.4% and 63.6%) and ISOLATION (52.9% and 44.1%) groups. Severe and critical cases were only present in the COVID (6.1% and 12.2%) and MIS-C (4.4% and 11.1%) groups; the severity difference was significant (p < 0.001). The groups did not differ significantly in the proportion of complete recovery and short- and long-term sequelae (p = 0.09). Conclusions: Patients with acute COVID-19 accounted for one-third of all ICU admissions, patients with MIS-C accounted for approximately another third, patients with worsening underlying disease accounted for 15%, and patients with incidental findings of SARS-CoV-2 positivity accounted for one-fifth of ICU admissions. A more significant disease was seen with acute COVID-19 and MIS-C.

• Publication type
• Journal Article MeSH

• MeSH
• Cardiovascular Diseases veterinary   MeSH
• Castration adverse effects   veterinary   MeSH
• Cats MeSH
• Comorbidity * MeSH
• Animal Feed MeSH
• Musculoskeletal Diseases veterinary   MeSH
• Overweight veterinary   MeSH
• Obesity * epidemiology   complications   veterinary   MeSH
• Human-Animal Bond MeSH
• Dogs MeSH
• Risk Factors * MeSH
• Animals MeSH
• Check Tag
• Cats MeSH
• Dogs MeSH
• Animals MeSH
• Publication type
• Review MeSH

The hotspot c.7541_7542delCT NOTCH1 mutation has been proven to have a negative clinical impact in chronic lymphocytic leukemia (CLL). However, an optimal method for its detection has not yet been specified. The aim of our study was to examine the presence of the NOTCH1 mutation in CLL using three commonly used molecular methods. Sanger sequencing, fragment analysis and allele-specific PCR were compared in the detection of the c.7541_7542delCT NOTCH1 mutation in 201 CLL patients. In 7 patients with inconclusive mutational analysis results, the presence of the NOTCH1 mutation was also confirmed using ultra-deep next generation sequencing. The NOTCH1 mutation was detected in 15% (30/201) of examined patients. Only fragment analysis was able to identify all 30 NOTCH1-mutated patients. Sanger sequencing and allele-specific PCR showed a lower detection efficiency, determining 93% (28/30) and 80% (24/30) of the present NOTCH1 mutations, respectively. Considering these three most commonly used methodologies for c.7541_7542delCT NOTCH1 mutation screening in CLL, we defined fragment analysis as the most suitable approach for detecting the hotspot NOTCH1 mutation.

• MeSH
• Leukemia, Lymphocytic, Chronic, B-Cell genetics   MeSH
• Humans MeSH
• Mutation * MeSH
• DNA Mutational Analysis methods   MeSH
• Peptide Fragments analysis   MeSH
• Receptor, Notch1 genetics   MeSH
• High-Throughput Nucleotide Sequencing MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Publikace poskytuje odborné veřejnosti a studentům VŠ informace o specifikách finančního řízení komerčních pojišťoven, výkonu dohledu v pojišťovnictví, zajišťovacích operacích, tvorbě a užití technických rezerv, metodách hodnocení výkonnosti pojišťoven a požadavcích směrnice Solvency II.

• MeSH
• Diagnostic Imaging methods   instrumentation   utilization   MeSH
• Fractures, Bone diagnosis   classification   radiography   MeSH
• Humans MeSH
• Elbow Joint radiography   MeSH
• Elbow Injuries MeSH
• Radiography methods   instrumentation   utilization   MeSH
• Fractures, Comminuted diagnosis   radiography   MeSH
• Check Tag
• Humans MeSH

• MeSH
• Diagnostic Imaging methods   instrumentation   utilization   MeSH
• Fractures, Bone diagnosis   classification   MeSH
• Humans MeSH
• Radiation Protection methods   standards   MeSH
• Radiography methods   instrumentation   utilization   MeSH
• Practice Guidelines as Topic MeSH
• Statistics as Topic MeSH
• Check Tag
• Humans MeSH

Publikace se zaměřuje na české zákony a právní předpisy, které se týkají znečištění životního prostředí hlukem. Určeno odborné veřejnosti.; Sborník stanovisek ochránce veřejných práv je zaměřen na ochranu veřejného zdraví před hlukem.

• MeSH
• Noise legislation & jurisprudence   MeSH
• Human Rights MeSH
• Jurisprudence MeSH
• Legislation as Topic MeSH
• Environmental Pollution legislation & jurisprudence   MeSH
• Publication type
• Collected Work MeSH
• Geographicals
• Czech Republic MeSH

• Conspectus
• Úkoly veřejné správy, správní opatření, legislativa
• NML Fields
• státní správa
• environmentální vědy

Příspěvky ze semináře pořádaného Ekumenickou akademií Praha zabývající se vztahem člověka a zvířete.

• MeSH
• Ethology MeSH
• Equine-Assisted Therapy history   MeSH
• Animal Assisted Therapy MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Collected Work MeSH

• Conspectus
• Sociální interakce. Sociální komunikace
• NML Fields
• humanitní vědy a umění
• NML Publication type
• semináře

• MeSH
• Animal Welfare MeSH
• Physical Therapy Modalities methods   MeSH
• Equine-Assisted Therapy MeSH

• Conspectus
• Fyzioterapie. Psychoterapie. Alternativní lékařství
• NML Fields
• rehabilitační a fyzikální medicína
• rehabilitační a fyzikální medicína
• NML Publication type
• informační publikace