The aim of this study was to investigate the use of a standardized animal model subjected to antibiotic treatment, and the effects of this treatment on the course of dextran sodium sulphate (DSS)-induced colitis in mice. By decontamination with selective antibiotics and observation of pathogenesis of ulcerative colitis (UC) induced chemically by exposure of mice to various concentrations of DSS, we obtained an optimum animal PGF model of acute UC manifested by mucin depletion, epithelial degeneration and necrosis, leading to the disappearance of epithelial cells, infiltration of lamina propria and submucosa with neutrophils, cryptitis, and accompanied by decreased viability of intestinal microbiota, loss of body weight, dehydration, moderate rectal bleeding, and a decrease in the selected markers of cellular proliferation and apoptosis. The obtained PGF model did not exhibit changes that could contribute to inflammation by means of alteration of the metabolic status and the induced dysbiosis did not serve as a bearer of pathogenic microorganisms participating in development of ulcerative colitis. The inflammatory process was induced particularly by exposure to DSS and its toxic action on compactness and integrity of mucosal barrier in the large intestine. This offers new possibilities of the use of this animal model in studies with or without participation of pathogenic microbiota in IBD pathogenesis.
- MeSH
- antibakteriální látky farmakologie MeSH
- apoptóza fyziologie MeSH
- epitelové buňky patologie MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- proliferace buněk fyziologie MeSH
- síran dextranu farmakologie MeSH
- střevní mikroflóra účinky léků fyziologie MeSH
- střevní sliznice mikrobiologie patologie MeSH
- ulcerózní kolitida chemicky indukované farmakoterapie patologie MeSH
- zánět farmakoterapie patologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
All twenty-nine PRRSV ORF7 nucleotide sequences obtained from clinical samples originating from the three Central European countries Austria (n = 7), Czech Republic (n = 12), and Slovakia (n = 10) belonged to type 1, subtype I (EU-1). Twenty-seven sequences encoded the typical length of the nucleocapsid protein composed of 128 amino acids. Two genetically identical ORF7s of PRRSV originating from a single farm in Slovakia showed a new length polymorphism of the nucleocapsid protein comprising 132 amino acids.
- MeSH
- fylogeneze MeSH
- genotyp MeSH
- lymfatické uzliny virologie MeSH
- molekulární sekvence - údaje MeSH
- nukleokapsida - proteiny genetika MeSH
- otevřené čtecí rámce MeSH
- plíce virologie MeSH
- polymorfismus genetický * MeSH
- prasata virologie MeSH
- reprodukční a respirační syndrom prasat virologie MeSH
- RNA virová krev MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční seřazení MeSH
- virus reprodukčního a respiračního syndromu prasat klasifikace genetika patogenita MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Rakousko MeSH
- Slovenská republika MeSH
The objective of our study was to develop and evaluate a TaqMan real-time RT-PCR (RRT-PCR) assay for universal detection of influenza A (IA) viruses. The primers and LNA-modified octanucleotide probe were selected to correspond to extremely conserved regions of the membrane protein (MP) segment identified by a comprehensive bioinformatics analysis including 10,405 IA viruses MP sequences, i.e., all of the sequences of the Influenza Virus Sequence database collected as of August 20, 2009. The RRT-PCR has a detection limit of approximately five copies of target RNA/reaction and excellent reaction parameters tested in four IA viruses reference laboratories. The inclusivity of the assay was estimated at both the bioinformatic and the experimental level. Our results predicted that this RRT-PCR assay was able to detect 99.5% of known human IA virus strains, 99.84% of pandemic influenza A (H1N1) strains, 99.75% of avian strains, 98.89% of swine strains, 98.15% of equine strains, and 100% of influenza A viruses of other origin.
- MeSH
- 5' nepřekládaná oblast genetika MeSH
- antilopy virologie MeSH
- financování organizované MeSH
- fylogeneze MeSH
- konzervovaná sekvence MeSH
- molekulární sekvence - údaje MeSH
- neutralizační testy MeSH
- pestivirové infekce veterinární virologie MeSH
- Pestivirus genetika izolace a purifikace klasifikace MeSH
- posttranslační úpravy proteinů MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční homologie MeSH
- virové proteiny MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- MeSH
- bovinní diarea virologie MeSH
- finanční podpora výzkumu jako téma MeSH
- virus bovinní diarey 1 genetika MeSH
- virus bovinní diarey 2 genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Geografické názvy
- Argentina MeSH
- Brazílie MeSH
- Egypt MeSH
- Finsko MeSH
- Slovenská republika MeSH
