A genetic etiology has been proposed for some severe forms of idiopathic male infertility and a region of the Y chromosome long arm (Yq) defined AZF is thought to be critical for the regulation of spermatogenesis. To date, several genes have been identified in AZF, but the actual relationship between genotype and phenotype related to AZF deletions is not well characterized. ICSI performed using spermatozoa of this deleted patiens will invariably pass this defect onto their male offspring. PGD for sex selection of preimplantation embryos is indicated in these patients. Material and methods: 762 azoospermic and severe oligozoospermic patiens from our IVF centrum were observed. Multiplex PCR strategy, 12 STSs in AZF region was used. Results: Microdeletions in AZF region were found in 15 men. Two pairs decided for PGD sex selection. Case report is presented in the oral presentation. Conclusions: Ethical and practical aspects of PGD indication for sex selection in Y-deleted men are discussed.
In the course of several past years, the question of healthcare quality was discussed from all angles, supported officially, and requested in some institutions; however, the level of the quality has often been defined chaotically, and it is not altogether clear what is to be expected from the various proposed systems. With the help of high-quality counselling, the team of the author's institution has introduced a system which from the start allowed setting up written and graphic working protocols based on the detailed analysis of all processes, definition of weak points and their reconstruction, and their following synthesis. These protocols are elaborated into detail horizontally and vertically in the form of processes, according to the needs of individual procedures, but in all of them the risk parts are clearly determined. For each risk point there is a definition of potential hazards, their solution, and the competent person responsible for their solution. The risk management system introduced in this way represents the most powerful instrument for providing healthcare in highest quality with minimizing the risk of damage to the patient. Regardless of other undeniable benefits (economic), the sole possibility to decrease the risk of patients‘ damage represents an unequivocal stimulus to introduce qualitative systems into the healthcare institutions.
- MeSH
- Aneuploidy MeSH
- Spindle Apparatus physiology MeSH
- Blastomeres cytology MeSH
- Photomicrography utilization MeSH
- Mitosis physiology genetics MeSH
- Publication type
- Abstracts MeSH
- MeSH
- Aneuploidy MeSH
- Spindle Apparatus physiology MeSH
- Blastomeres cytology MeSH
- Photomicrography utilization MeSH
- Mitosis physiology genetics MeSH
- Publication type
- Abstracts MeSH
