PURPOSE: We examined the effect of epidermal growth factor (EGF) and bone marrow transplantation (BMT) on gastrointestinal damage after high-dose irradiation of mice. MATERIAL AND METHODS: C57Black/6 mice were used. Two survival experiments were performed (12 and 13 Gy; (60)Co, 0.59-0.57 Gy/min). To evaluate BMT and EGF action, five groups were established - 0 Gy, 13 Gy, 13 Gy + EGF (at 2 mg/kg, first dose 24 h after irradiation and then every 48 h), 13 Gy + BMT (5 × 10(6) cells from green fluorescent protein [GFP] syngenic mice, 4 h after irradiation), and 13 Gy + BMT + EGF. Survival data, blood cell counts, gastrointestine and liver parameters and GFP positive cell migration were measured. RESULTS: BMT and EGF (three doses, at 2 mg/kg, administered 1, 3 and 5 days after irradiation) significantly increased survival (13 Gy). In blood, progressive cytopenia was observed with BMT, EGF or their combination having no improving effect early after irradiation. In gastrointestinal system, BMT, EGF and their combination attenuated radiation-induced atrophy and increased regeneration during first week after irradiation with the combination being most effective. Signs of systemic inflammatory reaction were observed 30 days after irradiation. CONCLUSIONS: Our data indicate that BMT together with EGF is a promising strategy in the treatment of high-dose whole-body irradiation damage.
- MeSH
- apoptóza účinky léků účinky záření MeSH
- celotělové ozáření škodlivé účinky MeSH
- epidermální růstový faktor aplikace a dávkování terapeutické užití MeSH
- experimentální radiační poranění farmakoterapie patologie terapie MeSH
- gastrointestinální trakt zranění patologie účinky záření MeSH
- kombinovaná terapie MeSH
- lithostatin účinky léků MeSH
- mitóza účinky léků účinky záření MeSH
- myši inbrední C57BL MeSH
- myši transgenní MeSH
- myši MeSH
- transplantace kostní dřeně * MeSH
- zánět patologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Aragonit nalezený při mineralogické analýze močových konkrementů je považován za velmi vzácný. Často je tento nález interpretován jako artefakt ve směsi dalších složek litiázy. Popisujeme případ pacientky se záchytem 16 konkrementů aragonitové urolitiázy. Jistou metabolickou poruchu se běžnými vyšetřovacími postupy nepodařilo zjistit, a proto metafylaxe a léčba selhávají.
Aragonite is considered to be rare in mineralogical analysis of urinary calculi. It's often interpreted as an artifact in a mixture of other crystals of the calculi. We present a case of patient with 16 stones formed by aragonite. Common investigative procedures failed to detect certain metabolic disorder and therefore metaphylaxis and treatment fail.
- MeSH
- diferenciální diagnóza MeSH
- lidé středního věku MeSH
- lidé MeSH
- lithostatin fyziologie nedostatek MeSH
- progrese nemoci MeSH
- uhličitan vápenatý moč MeSH
- urolitiáza * diagnóza etiologie komplikace terapie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Pancreatic stone protein (PSP)/regenerating protein 1-alpha (reg) is associated with inflammation, infection, and other disease-related stimuli. The prognostic value of PSP/reg among critically ill pediatric patients is unknown. The aim of this pilot study was to evaluate PSP/reg in children with systemic inflammatory response syndrome or sepsis. Prospective observational study, a five day evaluation period in children 0-19years old with systemic inflammatory response syndrome or septic state. Blood tests to determine levels of PSP/reg were obtained as long as the patient met the criteria for systemic inflammatory response syndrome or sepsis. PSP/reg levels did not differ between patients with systemic inflammatory response syndrome and septic condition until organ dysfunction signs were present. PSP/reg levels were significantly higher in patients with a PELOD score of 12 or higher or in those with MODS. Patients who died tended to have higher PSP/reg levels.
- MeSH
- biologické markery krev MeSH
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- lithostatin krev MeSH
- mladiství MeSH
- mladý dospělý MeSH
- multiorgánové selhání krev mortalita patologie MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- prognóza MeSH
- prospektivní studie MeSH
- sepse krev mortalita patologie MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- pozorovací studie MeSH
BACKGROUND: Reg-1alpha plays a role in various types of tissue regeneration. AIM: To evaluate serum Reg-1alpha for the diagnosis of metabolic syndrome. METHODS: 14 non-obese, healthy subjects and 15 individuals with metabolic syndrome were studied. Anthropometric and laboratory analysis in sera (Body Mass Index--BMI, insulin, triglycerides, cholesterol, HDL-cholesterol, LDL-cholesterol, uric acid, aglucose, Quicki calculation, Reg-1alpha) were performed. RESULTS: Reg-1alpha levels did not differ between subjects with metabolic syndrome and healthy subjects (means 597.7 vs. 631.1 ng/l, p < 0.01) and positively correlated only with fasting glucose (r = 0.34; p < 0.01) and age (r = 0.38; p < 0.01); Reg-1alpha correlated even after adjustment to age. Reg-lalpha concentrations did not differ in men and women. CONCLUSIONS: Our study, for the first time, indicates that serum Reg-1alpha is not useful for the diagnosis of metabolic syndrome (Tab. 3, Ref. 7).
BACKGROUND: The need for a laboratory marker of myocardial ischemia has been alluded to for at least the last decade. AIM: The aim of this study was to evaluate the diagnostic importance of the myosin light chain-1 (MLC-1), clusterin and Reg-Ialpha in patients with suspected myocardial ischemia. METHODS: A group of 176 at high-risk for myocardial ischemia subjects was evaluated and divided into two subgroups using myocardial SPECT (Single Photon Emission Computed Tomography) - individuals with and without signs of myocardial ischemia. Laboratory markers in venous blood were repeatedly examined in all subjects: a) immediately prior to SPECT: C-reactive protein, Haemoglobin, Hematocrite, Lactate, MLC-1, Clusterin, Reg-Ialpha b) at subjective maximum: Hb, Htc, lactate, MLC-1, Clusterin, Reg-Ialpha c) 30 min after stress levels reached their peak: MLC-1, Clusterin, Reg-Ialpha and d) 60 min after peak stress levels: MLC-1, Clusterin, Reg-Ialpha. RESULTS: Patients were divided into subgroups according to their positive and negative SPECT results (positive: n = 37; negative: n = 139). MLC-1 values were different for all 4 blood collections. An increase in MLC-1 > 2.2 mg/l showed 64 % sensitivity and 88 % specificity for the diagnosed presence of myocardial ischemia (AUC 0.81; LR+ 5.9; PPV+ 68 % and NPV- 87 %). There was no significant difference between the groups in terms of Clusterin and Reg-Ialpha for any of the sampling periods. CONCLUSIONS: High diagnostic efficacy of detectable MLC-1 was shown for the diagnosis of latent myocardial ischemia. Measurement of serum Clusterin or Reg-Ialpha did not sufficient for the diagnosis of latent myocardial ischemia.
- MeSH
- biologické markery krev MeSH
- ischemická choroba srdeční diagnóza MeSH
- jednofotonová emisní výpočetní tomografie MeSH
- klusterin krev MeSH
- lehké řetězce myosinu krev MeSH
- lidé MeSH
- lithostatin krev MeSH
- senzitivita a specificita MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
The commonly used laboratory markers of coronary involvement in subjects with acute coronary syndrome (ACS) are not yet myocardial ischemia-specific and show a late irreversible involvement of the myocardium. A laboratory test has been searched for in order to distinguish persons with myocardial ischemia and typical CAD symptoms to CAD-free individuals. Reg-Ialpha is the product of Reg-I gene which plays a significant role in myocardial regeneration. 38 individuals with suspicion of acute coronary syndrome were tested on admission, after 2 and 6 hours. In all of them cardiac troponin I, myoglobin, C-reactive protein (CRP) and Reg-I alpha were analysed. Our findings did not support the hypothesis that measurement of Reg-Ia maybe the useful marker of myocardial stress.
- MeSH
- biologické markery krev MeSH
- C-reaktivní protein analýza MeSH
- infarkt myokardu diagnóza MeSH
- lidé MeSH
- lithostatin krev MeSH
- myoglobin krev MeSH
- nestabilní angina pectoris diagnóza MeSH
- senioři MeSH
- troponin I krev MeSH
- Check Tag
- lidé MeSH
- senioři MeSH
- Publikační typ
- časopisecké články MeSH
