BACKGROUND: The COMBO stent (OrbusNeich Medical, Ft. Lauderdale, Florida) is a new-generation bio-engineered drug eluting stent, combining an abluminal coating of a bioabsorbable polymer matrix for sustained release of sirolimus and luminal anti-CD34 coating for endothelial progenitor cell capture and rapid endothelialization. METHODS: The Multinational Abluminal Sirolimus Coated BiO-Engineered StenT (MASCOT) registry was a prospective post-marketing study conducted from June 2014-May 2017 across 60 centers globally. Patients were eligible if COMBO stent implantation was attempted, and they received dual antiplatelet therapy (DAPT) per local guidelines. Follow-up was conducted by trained research staff at 1, 6 and 12 months by phone or clinic visit to capture clinical events and DAPT cessation events. The primary endpoint was 1-year target lesion failure (TLF), composite of cardiac death, non-fatal myocardial infarction not clearly attributable to a non-target vessel, or ischemia-driven target lesion revascularization. RESULTS: A total of 2614 patients were enrolled over the study period with 96.7% completion of 1-year follow-up. The mean age of enrolled patients was 62.9 ± 11.2 years and 23.0% were female. Diabetes mellitus was present at baseline in 33.5%. A total of 56.1% patients underwent PCI for acute coronary syndrome (ACS). The 1-year primary endpoint of TLF occurred in 3.4% patients (n = 88). Definite stent thrombosis occurred in 0.5% patients (n = 12). CONCLUSION: The MASCOT post marketing registry provides comprehensive safety and efficacy outcomes following contemporary PCI using the novel COMBO stent in an all-comer population. This platform is associated with low rates of 1-year TLF and ST. CLINICALTRIALS. GOV IDENTIFIER: NCT02183454.
- MeSH
- Bioengineering methods MeSH
- Time Factors MeSH
- Immunosuppressive Agents pharmacology MeSH
- Percutaneous Coronary Intervention methods MeSH
- Middle Aged MeSH
- Humans MeSH
- Follow-Up Studies MeSH
- Coronary Artery Disease surgery MeSH
- Product Surveillance, Postmarketing statistics & numerical data MeSH
- Prosthesis Design MeSH
- Registries * MeSH
- Retrospective Studies MeSH
- Sirolimus pharmacology MeSH
- Drug-Eluting Stents * MeSH
- Treatment Outcome MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Multicenter Study MeSH
- Observational Study MeSH
The porous polymer foams act as a template for neotissuegenesis in tissue engineering, and, as a reservoir for cell transplants such as pancreatic islets while simultaneously providing a functional interface with the host body. The fabrication of foams with the controlled shape, size and pore structure is of prime importance in various bioengineering applications. To this end, here we demonstrate a thermally induced phase separation (TIPS) based facile process for the fabrication of polymer foams with a controlled architecture. The setup comprises of a metallic template bar (T), a metallic conducting block (C) and a non-metallic reservoir tube (R), connected in sequence T-C-R. The process hereinafter termed as Dip TIPS, involves the dipping of the T-bar into a polymer solution, followed by filling of the R-tube with a freezing mixture to induce the phase separation of a polymer solution in the immediate vicinity of T-bar; Subsequent free-drying or freeze-extraction steps produced the polymer foams. An easy exchange of the T-bar of a spherical or rectangular shape allowed the fabrication of tubular, open- capsular and flat-sheet shaped foams. A mere change in the quenching time produced the foams with a thickness ranging from hundreds of microns to several millimeters. And, the pore size was conveniently controlled by varying either the polymer concentration or the quenching temperature. Subsequent in vivo studies in brown Norway rats for 4-weeks demonstrated the guided cell infiltration and homogenous cell distribution through the polymer matrix, without any fibrous capsule and necrotic core. In conclusion, the results show the "Dip TIPS" as a facile and adaptable process for the fabrication of anisotropic channeled porous polymer foams of various shapes and sizes for potential applications in tissue engineering, cell transplantation and other related fields.
- MeSH
- Bioengineering methods MeSH
- Time Factors MeSH
- Calorimetry, Differential Scanning MeSH
- Microscopy, Electron, Scanning MeSH
- Molecular Weight MeSH
- Polymers chemistry MeSH
- Porosity MeSH
- Rats, Inbred BN MeSH
- Surface Properties MeSH
- Mercury analysis MeSH
- Temperature * MeSH
- Tissue Scaffolds chemistry MeSH
- Phase Transition * MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
In contemporary science, the analysis of human walking is extensively used. The prediction of leg motion, as well as rehabilitation, can be usable for orthosis and prosthesis programing. Our work is focused on predicting of human walking by angle-angle diagrams, also called cyclograms. The applications of cyclograms in conjunction with artificial intelligence offers wide area of applications in medicine. But until now, this approach has not been studied or applied in practice.
- MeSH
- Principal Component Analysis MeSH
- Leg physiology MeSH
- Bioengineering methods MeSH
- Models, Biological MeSH
- Biomechanical Phenomena MeSH
- Walking physiology MeSH
- Infrared Rays MeSH
- Knee physiology MeSH
- Ankle physiology MeSH
- Hip physiology MeSH
- Humans MeSH
- Neural Networks, Computer MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Enzymy jsou jako biokatalyzátory s prospěchem využívány v mnoha průmyslových odvětvích. Použití enzymových technologií je omezeno nízkou stabilitou enzymů při technologických výrobních procesech. Na enzymy jsou kladeny vysoké nároky z hlediska jejich stability (pn skladováni teplotní a v rozpouštědlech), katalytické aktivity a specifity. Často jsou potřeba enzymy s novými vlastnostmi Nejdéle používaným způsobem zvýšení stability enzymů je imobilizace, dále lze použít stabilizační činidla nebo kovalentní modifikace. Vlastnosti enzymů lze také částečně ovlivnit změnou podmínek, v nichž probíhá reakce, může to být např. změna rozpouštědla či tlaku. Novějšími metodami umožňujícími získat enzymy s novými vlastnostmi jsou rekombinantní techniky využívající postupy racionální nebo kombinatorické.
Enzymes as biocatalysts are used with benefit in many industrial processes. The use of enzyme technology is limited by the low stability of enzymes in technological production processes. The technological processes are demanding in terms of enzyme stability (during storage, temperature stabiirty and stability in orgnic solvents), catalytic activity and specificity. There are often needs for enzymes with new properties. The longest used way to enhance the stability of the enzyme is immobilization, also the stabilizing agents can be used, or covalent modifications. Properties of enzymes may also be partially affected by changing conditions in which the reaction takes place, a solvent or pressure can be changed for example. Newer methods which allow production of enzymes with new properties are recombinant techniques using rational and combinatorial methods.
