AIMS: This review article focuses on electrochemical biosensors in the diagnosis of diabetes mellitus and their latest trends and advances. In particular, non-enzymatic, non-invasive, wearable, and non-glucose biosensors are described. METHODS: The current literature was searched and recent works on this matter were cited and discussed in the text of this paper. RESULTS: The overworld health problem, the incurable disease, the global burden on health insurers and society, and above all one of the leading causes of death – all characterize diabetes mellitus, a lifelong chronic disease that affects hundreds of millions of people around the world. The new types of biosensors bring new opportunities in the care of diabetic patients and improve current methods. The practical relevance of the recent fi ndings is expected in medicine in next years. CONCLUSIONS: The authors summarized the modern possibilities of biosensing, their pros and cons, and their perspectives for the future. The discussion outcome from the current literature (Tab. 4, Fig. 1, Ref. 63).
- MeSH
- biosenzitivní techniky * klasifikace metody statistika a číselné údaje trendy MeSH
- diabetes mellitus * diagnóza prevence a kontrola MeSH
- glykovaný hemoglobin analýza MeSH
- inzulin lidský analýza MeSH
- krevní glukóza analýza MeSH
- lidé MeSH
- lidský sérový albumin analýza MeSH
- nanostruktury analýza klasifikace statistika a číselné údaje terapeutické užití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
There is increased interest in the investigation and implementation of rapid screening methods for detection of pesticide residues. This study reports development of an immunostrip test for thiabendazole detection based on indirect competitive principle using carbon particles as a label. Nitrocellulose membrane strip was coated with a thiabendazole-protein conjugate in the defined test zone. In flow of an antibody-carbon complex and thiabendazole along the strip, the intensity of black colour formed in the test line reflected the thiabendazole concentration and semi-quantitative estimation could be carried out visually. The optimized test was accomplished within 10 min and the visual detection limit was achieved 0.25 ng mL(-1) of standard sample. Moreover, immunostrip was evaluated quantitatively using scanning densitometry. Based on standard curve, the detection limit of the proposed test was as low as 0.08+/-0.03 ng mL(-1) with an IC(50) value of 0.60+/-0.08 ng mL(-1) and a linear working range of 0.11-4.13 ng mL(-1). Results of testing precision, stability, and specificity demonstrated that the assay provided a reliable performance. This immunostrip was applied to analysis of spiked fruit juices in range of 0.05-5 mg L(-1). Matrix interferences were avoided by simple dilution of samples. Both visual and instrumental evaluations indicated a good agreement with results obtained by ELISA. Recoveries from juices were from 81.9 to 123.6% and relative standard deviations ranged from 9.9 to 19.3%. The developed strip offers potential as a useful rapid and simple method for screening of thiabendazole in fruit juices at levels far below the maximum residue limits.
- MeSH
- biosenzitivní techniky přístrojové vybavení normy statistika a číselné údaje MeSH
- ELISA MeSH
- imunoanalýza přístrojové vybavení normy statistika a číselné údaje MeSH
- koloidy MeSH
- kontaminace potravin analýza MeSH
- lidé MeSH
- maximální přípustná koncentrace MeSH
- nápoje analýza MeSH
- ovoce chemie MeSH
- protilátky MeSH
- rezidua pesticidů analýza imunologie MeSH
- thiabendazol analýza imunologie MeSH
- uhlík MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
