Bazocelulárny karcinóm je jednou z celosvetovo najčastejšie sa vyskytujúcich malignít. V indikácii lokálnej liečby bazocelulárneho karcinómu zohráva centrálnu úlohu imiquimod a fotodynamická terapia. Uvedené modality sú rezervované pre superficiálne nízkorizikové podtypy, čo významne redukuje mieru ich využiteľnosti v klinickej praxi. S úmyslom preklenutia súčasných limitácií prebieha v oblasti lokálnej liečby bazocelulárneho karcinómu rozsiahly výskum. Predkladaný prehľad stručne sumarizuje aktuálne terapeutické možnosti, ich obmedzenia a príklady nových prístupov v liečbe tohto vysoko prevalentného ochorenia.
Basal cell carcinoma is one of the most common malignancies worldwide. In the indication of topical treatment of basal cell carcinoma, imiquimod and photodynamic therapy play a central role. These modalities are reserved for low-risk superficial subtypes, which reduces their degree of applicability in clinical practice. With the intention of overcoming current limitations, extensive research is conducted in the field of topical treatment of basal cell carcinoma. This article briefly summarizes the current therapeutic modalities, their limitations, and examples of new approaches in the treatment of this highly prevalent disease.
Today, researchers have focused on the application of environmentally-benign and sustainable micro- and nanosystems for drug delivery and cancer therapy. Compared to conventional chemotherapeutics, advanced micro- and nanosystems designed by applying abundant, natural, and renewable feedstocks have shown biodegradability, biocompatibility, and low toxicity advantages. However, important aspects of toxicological assessments, clinical translational studies, and suitable functionalization/modification still need to be addressed. Herein, the benefits and challenges of green nanomedicine in cancer nanotherapy and targeted drug delivery are cogitated using nanomaterials designed by exploiting natural and renewable resources. The application of nanomaterials accessed from renewable natural resources, comprising metallic nanomaterials, carbon-based nanomaterials, metal-organic frameworks, natural-derived nanomaterials, etc. for targeted anticancer drug delivery and cancer nanotherapy are deliberated, with emphasis on important limitations/challenges and future perspectives.
- MeSH
- lékové transportní systémy MeSH
- lidé MeSH
- nádory * farmakoterapie MeSH
- nanomedicína MeSH
- nanostruktury * terapeutické užití MeSH
- přírodní zdroje MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Bazocelulárny karcinóm je jednou z celosvetovo najčastejšie sa vyskytujúcich malignít. V indikácii lokálnej liečby bazocelulárneho karcinómu zohráva centrálnu úlohu imiquimod a fotodynamická terapia. Uvedené modality sú rezervované pre superficiálne nízkorizikové podtypy, čo významne redukuje mieru ich využiteľnosti v klinickej praxi. S úmyslom preklenutia súčasných limitácií prebieha v oblasti lokálnej liečby bazocelulárneho karcinómu rozsiahly výskum. Predkladaný prehľad stručne sumarizuje aktuálne terapeutické možnosti, ich obmedzenia a príklady nových prístupov v liečbe tohto vysoko prevalentného ochorenia.
Basal cell carcinoma is one of the most common malignancies worldwide. In the indication of topical treatment of basal cell carcinoma, imiquimod and photodynamic therapy play a central role. These modalities are reserved for low-risk superficial subtypes, which reduces their degree of applicability in clinical practice. With the intention of overcoming current limitations, extensive research is conducted in the field of topical treatment of basal cell carcinoma. This article briefly summarizes the current therapeutic modalities, their limitations, and examples of new approaches in the treatment of this highly prevalent disease.
AIMS: This review article focuses on electrochemical biosensors in the diagnosis of diabetes mellitus and their latest trends and advances. In particular, non-enzymatic, non-invasive, wearable, and non-glucose biosensors are described. METHODS: The current literature was searched and recent works on this matter were cited and discussed in the text of this paper. RESULTS: The overworld health problem, the incurable disease, the global burden on health insurers and society, and above all one of the leading causes of death – all characterize diabetes mellitus, a lifelong chronic disease that affects hundreds of millions of people around the world. The new types of biosensors bring new opportunities in the care of diabetic patients and improve current methods. The practical relevance of the recent fi ndings is expected in medicine in next years. CONCLUSIONS: The authors summarized the modern possibilities of biosensing, their pros and cons, and their perspectives for the future. The discussion outcome from the current literature (Tab. 4, Fig. 1, Ref. 63).
- MeSH
- biosenzitivní techniky * klasifikace metody statistika a číselné údaje trendy MeSH
- diabetes mellitus * diagnóza prevence a kontrola MeSH
- glykovaný hemoglobin analýza MeSH
- inzulin lidský analýza MeSH
- krevní glukóza analýza MeSH
- lidé MeSH
- lidský sérový albumin analýza MeSH
- nanostruktury analýza klasifikace statistika a číselné údaje terapeutické užití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Bacterial nanocellulose (BNC) is produced by some bacterial strains to enable them to keep on the surface of cultural media. Compared to “common” cellulose, BNC becomes even more popular for its higher crystallinity and better mechanical properties. It can be used in a broad spectrum of industrial applications. This work describes the structure, properties, and origin of BNC together with factors that can affect the growth of BNC in a laboratory. Possible usage in industry, particularly in various medical applications, such as wound healing, is also discussed.
- Klíčová slova
- bakteriální nanocelulosa,
- MeSH
- anaerobní bakterie MeSH
- celulosa * biosyntéza terapeutické užití MeSH
- hojení ran MeSH
- kultivační techniky metody MeSH
- lidé MeSH
- nanostruktury terapeutické užití MeSH
- tkáňové inženýrství MeSH
- zpracovatelský průmysl MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Závěrečná zpráva o řešení grantu Agentury pro zdravotnický výzkum MZ ČR
Nestr.
The artificial skin substitutes are applied in the treatment of chronic skin defects and acute wounds to control wound healing process. However, a significant problem is their high price and risks originated from infectious complications. Whereas bilayer sponge Integra involves two step surgery procedure, the monolayer Matriderm applicable in one step is not approved in Czech. Our proposed 3D nanostructured bio-substitute is consisted of antibacterial collagen/chitosan porous sponge (dermal substitute) doped with hyperstable fibroblast growth factor FGF2 and covered with elastic nanofiber layer (dermal-epidermal junction). The dermal substitute layer facilitates ingrowth of native fibroblasts and blood vessels while nanoporous mucoadhesive membrane simulates the “glue-like” interlayer ensuring good adhesion to the epidermis autograft. In this project, proposed bio-substitute will be evaluated physico-chemically, biomechanically, in-vitro on fibroblasts, keratinocytes and macrophages followed by treatment of full thickness skin defect in preclinical study on experimental pig model.
Umělé kožní náhrady řídící proces hojení ran se používají v léčbě chronických kožních defektů a akutních ran. Závažným problémem je jejich vysoká cena a rizika pocházející z infekčních komplikací. Zatímco dvojvrstevná pěna Integra vyžaduje dvoukrokový chirurgický postup, jenovrstevný Matriderm použitelný v jednom kroku není schválen v ČR. Námi navrhovaná 3D nanostrukturní bio-náhrada se skládá z antibakteriální kolagen/chitosanové porézní pěny (dermální náhrada) s přísadou hyperstabilního fibroblastového růstového faktoru FGF2 a potažená elastickou nanovlákennou vrstvou (dermálně-epidermální spoj). Vrstva dermální náhrady umožňuje prorůstání nativních fibroblastů a cév, zatímco nanoporézní mukoadhezivní membrána simuluje "lepidlovou" mezivrstvu zajišťující dobrou přilnavost k epidermálnímu autograftu. V rámci tohoto projektu bude navržená bio-náhrada hodnocena fyzikálně-chemicky, biomechanicky, in-vitro pomocí fibroblastů, keratinocytů a makrofágů a následně použita na hojení defektu kůže v celé jeho tloušťce v preklinické studii na experimentálním modelu prasete.
- MeSH
- biokompatibilní materiály terapeutické užití MeSH
- biopolymery terapeutické užití MeSH
- fibroblastový růstový faktor 2 terapeutické užití MeSH
- hojení ran MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- nanostruktury terapeutické užití MeSH
- prasata MeSH
- protézy a implantáty MeSH
- tkáně MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- hodnotící studie MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- technika lékařská, zdravotnický materiál a protetika
- chirurgie
- NLK Publikační typ
- závěrečné zprávy o řešení grantu AZV MZ ČR
Skin is the external part of the human body; thus, it is exposed to outer stimuli leading to injuries and damage, due to being the tissue mostly affected by wounds and aging that compromise its protective function. The recent extension of the average lifespan raises the interest in products capable of counteracting skin related health conditions. However, the skin barrier is not easy to permeate and could be influenced by different factors. In the last decades an innovative pharmacotherapeutic approach has been possible thanks to the advent of nanomedicine. Nanodevices can represent an appropriate formulation to enhance the passive penetration, modulate drug solubility and increase the thermodynamic activity of drugs. Here, we summarize the recent nanotechnological approaches to maintain and replace skin homeostasis, with particular attention to nanomaterials applications on wound healing, regeneration and rejuvenation of skin tissue. The different nanomaterials as nanofibers, hydrogels, nanosuspensions, and nanoparticles are described and in particular we highlight their main chemical features that are useful in drug delivery and tissue regeneration.
- MeSH
- fyziologie kůže účinky léků MeSH
- kůže metabolismus MeSH
- lékové transportní systémy * MeSH
- lidé MeSH
- nanostruktury terapeutické užití MeSH
- omlazení * MeSH
- regenerace účinky léků MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Dyslipidemias are lipid metabolism alterations that cause increased levels of serum lipoprotein, cholesterol, and triglycerides. These alterations are associated with a higher incidence of cardiovascular diseases and are a risk factor for atherosclerosis development. This study aimed to evaluate the effect of Rosmarinus officinalis essential oil (EORO, 100 mg/kg) and its nanoemulsion (NEORO, 500 µg/kg) on Triton and coconut saturated-fat-induced (CSF) dyslipidemias using Wistar rats. The phytochemical evaluation of EORO performed by gas chromatography-mass spectroscopy (GC-MS) revealed 1,8-cineole (33.70%), camphor (27.68%), limonene (21.99%), and α-pinene (8.13%) as its major compounds. Triton-induced dyslipidemia significantly increased total cholesterol, LDL, and triglycerides levels. On the other hand, the groups treated with EORO and NEORO had significantly reduced total cholesterol, LDL, and triglycerides compared to the group treated only with Triton. Similar results were observed on the positive control treated with simvastatin. Dyslipidemia induced with coconut saturated-fat (CSF) caused abdominal fat gain, hypercholesterolemia, hypertriglyceridemia, increased LDL levels, and atherogenesis in the aorta. In contrast, the groups treated with EORO, NEORO, and simvastatin had significantly reduced hypercholesterolemia and hypertriglyceridemia, reduced abdominal fat gain, and absence of atherogenesis in the vascular endothelium. Overall, in the Triton-induced dyslipidemia model, EORO treatment had superior values than NEORO's (and simvastatin), although the differences were not too high, while in the CSF model, the values were mixed. In this manner, our results show an anti-dyslipidemic and anti-atherogenic activity effect by EORO and NEORO.
- Klíčová slova
- nanoemulze,
- MeSH
- aorta thoracica diagnostické zobrazování patologie MeSH
- ateroskleróza farmakoterapie komplikace MeSH
- chromatografie statistika a číselné údaje MeSH
- dyslipidemie * farmakoterapie komplikace MeSH
- mikroskopie elektronová rastrovací MeSH
- modely u zvířat MeSH
- nanostruktury statistika a číselné údaje terapeutické užití MeSH
- oleje prchavé * analýza aplikace a dávkování chemie MeSH
- potkani Wistar MeSH
- rozmarýn MeSH
- simvastatin terapeutické užití MeSH
- tuky škodlivé účinky MeSH
- výsledek terapie MeSH
- Publikační typ
- klinická studie MeSH
- práce podpořená grantem MeSH
Hafnium dioxide (HfO2) is attracting attention for bio-related applications due to its good cytocompatibility, high density, and resistance to corrosion and mechanical damage. Here we synthesize two types of hafnium-oxide thin films on substrates via self-organized electrochemical anodization: (1) an array of hierarchically structured nanorods anchored to a thin oxide layer and (2) a microscopically flat oxide film. The nanostructured film is composed of a unique mixture of HfO2, suboxide Hf2O3, and oxide-hydroxide compound HfO2·nH2O whereas the flat film is mainly HfO2. In vitro interaction of the two films with MG-63 osteoblast-like cells and Gram-negative E. coli bacteria is studied for the first time to assess the potential of the films for biomedical application. Both films reveal good cytocompatibility and affinity for proteins, represented by fibronectin and especially albumin, which is absorbed in a nine times larger amount. The morphology and specific surface chemistry of the nanostructured film cause a two-fold enhanced antibacterial effect, better cell attachment, significantly improved proliferation of cells, five-fold rise in the cellular Young's modulus, slightly stronger production of reactive oxygen species, and formation of cell clusters. Compared with the flat film, the nanostructured one features the weakening of AFM-measured adhesion force at the cell/surface interface, probably caused by partially lifting the nanorods from the substrate due to the strong contact with cells. The present findings deepen the understanding of biological processes at the living cell/metal-oxide interface, underlying the role of surface chemistry and the impact of nanostructuring at the nanoscale.
- MeSH
- biokompatibilní materiály farmakologie MeSH
- buněčné linie MeSH
- Escherichia coli účinky léků MeSH
- hafnium * chemie farmakologie MeSH
- lidé MeSH
- nanostruktury chemie terapeutické užití MeSH
- osteoblasty cytologie účinky léků MeSH
- oxidy * chemie farmakologie MeSH
- povrchové vlastnosti MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The nanofiber scent carriers prepared by electrospinning from poly-vinyl-butyral (PVB), poly-ε-polycaprolactone (PCL), poly-vinyl- alcohol (PVA) and nylon 6/6 were tested as materials for collecting and preserving cigarette tobacco olfactory trace. Nanofiber material can include polar groups on its surface that influence wettability and also attracting of specific molecules creating olfactory trace. Nanofiber material and Aratex were also morphologicaly compared by scanning electron microscope (SEM) where nanofiber carrier had from twenty-two times to thirty-six times smaller fibre diameter than Aratex fibres. Olfactory testing of nanofiber scent carriers were compared to Aratex as traditionally used material for collecting of olfactory traces in criminology. Olfactory tests were carried out by dogs with special training and by mass spectrometer. Olfactory tests carried out by dogs clearly proved that nanofiber scent carriers were able to collect and preserve olfactory trace of lower concentration despite of their lower weight compared to samples of Aratex material. Olfactory tests carried out by mass spectrometer affirmed better olfactory properties of nanofiber scent carriers compared to Aratex, when nanofiber scent carriers were able to preserve 9 of 14 specific molecules characteristic for cigarette tobacco compared to Aratex that was able to preserve only 5 of 14 specific molecules characteristic for cigarette tobacco. The experiments with olfactory trace detection with materials with a comparable mass are in progress.
