Bacterial nanocellulose (BNC) is produced by some bacterial strains to enable them to keep on the surface of cultural media. Compared to “common” cellulose, BNC becomes even more popular for its higher crystallinity and better mechanical properties. It can be used in a broad spectrum of industrial applications. This work describes the structure, properties, and origin of BNC together with factors that can affect the growth of BNC in a laboratory. Possible usage in industry, particularly in various medical applications, such as wound healing, is also discussed.

• Klíčová slova
• bakteriální nanocelulosa,
• MeSH
• anaerobní bakterie MeSH
• celulosa * biosyntéza   terapeutické užití   MeSH
• hojení ran MeSH
• kultivační techniky metody   MeSH
• lidé MeSH
• nanostruktury terapeutické užití   MeSH
• tkáňové inženýrství MeSH
• zpracovatelský průmysl MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH

The growing interest in a healthy lifestyle and in environmental protection is changing habits regarding food consumption and agricultural practices. Good agricultural practice is indispensable, particularly for raw vegetables, and can include the use of plant probiotic bacteria for the purpose of biofertilization. In this work we analysed the probiotic potential of the rhizobial strain PEPV40, identified as Rhizobium laguerreae through the analysis of the recA and atpD genes, on the growth of spinach plants. This strain presents several in vitro plant growth promotion mechanisms, such as phosphate solubilisation and the production of indole acetic acid and siderophores. The strain PEPV40 produces cellulose and forms biofilms on abiotic surfaces. GFP labelling of this strain showed that PEPV40 colonizes the roots of spinach plants, forming microcolonies typical of biofilm initiation. Inoculation with this strain significantly increases several vegetative parameters such as leaf number, size and weight, as well as chlorophyll and nitrogen contents. Therefore, our findings indicate, for the first time, that Rhizobium laguerreae is an excellent plant probiotic, which increases the yield and quality of spinach, a vegetable that is increasingly being consumed raw worldwide.

• MeSH
• biofilmy MeSH
• celulosa biosyntéza   MeSH
• fenotyp MeSH
• fylogeneze MeSH
• kořeny rostlin mikrobiologie   MeSH
• probiotika * MeSH
• Rhizobium klasifikace   fyziologie   MeSH
• semenáček mikrobiologie   MeSH
• Spinacia oleracea růst a vývoj   mikrobiologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Stereospecific nucleation of mesoporous hybrid microspheres composed of CaCO3 and carrageenan was appended to one side of bacterial cellulose membrane synthesized in static cultures of Gluconacetobacter hansenii to develop an implantable drug delivery device. The synthesis of the hybrid microparticles proceeds by self-assembly mechanism in the presence of calcium and contains tailorable amounts of doxorubicin. However, in the absence of the particles, doxorubicin was distributed along the BC film, but without control release of drug. Infrared spectroscopy, confocal and scanning electron microscopies analyses demonstrate that the doxorubicin is entrapped inside the hybrid particles with approximately 80% drug loading compared to the 11% obtained for native bacterial cellulose. Doxorubicin content in the hybrid particles can be increased by a factor of 10 (from 258.6 to 2586.3 nmol ml−1), and also by the quantities of particles regulated by the CaCO3–carrageenan content and the physicochemical microenvironment. The hybrid BC system can be considered as smart device since the kinetic release of doxorubicin from the hybrid cellulose system rise from 1.50 to 2.75 μg/membrane/day when the pH decreases from 7.4 to 5.8 at 37 °C, a pathologic simulated environment. The hybrid microparticle system can be potentially used as an implantable drug delivery system for personalized oncological therapies.

• MeSH
• antitumorózní látky aplikace a dávkování   terapeutické užití   toxicita   MeSH
• bakteriální polysacharidy biosyntéza   farmakologie   metabolismus   MeSH
• biomedicínský výzkum metody   trendy   MeSH
• celulosa * biosyntéza   farmakologie   MeSH
• doxorubicin aplikace a dávkování   farmakologie   MeSH
• elektronová mikroskopie metody   využití   MeSH
• Gluconacetobacter růst a vývoj   účinky léků   MeSH
• individualizovaná medicína metody   trendy   využití   MeSH
• karagenan * biosyntéza   metabolismus   MeSH
• konfokální mikroskopie metody   využití   MeSH
• lidé MeSH
• nanokompozity chemie   mikrobiologie   využití   MeSH
• spektrofotometrie infračervená metody   využití   MeSH
• statistika jako téma metody   MeSH
• systémy cílené aplikace léků metody   využití   MeSH
• techniky in vitro MeSH
• uhličitan vápenatý * chemická syntéza   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH

• MeSH
• Acanthamoeba * parazitologie   patogenita   růst a vývoj   účinky léků   MeSH
• akantamébová keratitida diagnóza   etiologie   parazitologie   patologie   prevence a kontrola   přenos   MeSH
• celulosa-1,4-beta-cellobiosidasa izolace a purifikace   MeSH
• celulosa biosyntéza   MeSH
• glukosa metabolismus   nedostatek   MeSH
• glykogen metabolismus   sekrece   MeSH
• glykogenfosforylasa MeSH
• lidé MeSH
• malá interferující RNA aplikace a dávkování   MeSH
• prospektivní studie MeSH
• růst a vývoj fyziologie   účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH