Surface plasmon resonance microscopy and imaging are optical methods that enable observation and quantification of interactions of nano- and microscale objects near a metal surface in a temporally and spatially resolved manner. This review describes the principles of surface plasmon resonance microscopy and imaging and discusses recent advances in these methods, in particular, in optical platforms and functional coatings. In addition, the biological applications of these methods are reviewed. These include the detection of a broad variety of analytes (nucleic acids, proteins, bacteria), the investigation of biological systems (bacteria and cells), and biomolecular interactions (drug-receptor, protein-protein, protein-DNA, protein-cell).

• MeSH
• Bacteria isolation & purification   ultrastructure   MeSH
• Equipment Design MeSH
• Humans MeSH
• Protein Interaction Mapping instrumentation   methods   MeSH
• Microscopy instrumentation   methods   MeSH
• Nucleic Acids analysis   MeSH
• Surface Plasmon Resonance instrumentation   methods   MeSH
• Proteins analysis   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Review MeSH

Piezoelectric quartz crystals serve as resonator-based transducers for direct and real-time monitoring of immunoaffinity interactions. The measuring system is briefly characterized; several examples for immobilization of antibodies are recommended. The piezoelectric immunoassays employing direct, competitive, and displacement-based formats are demonstrated on examples. The method for kinetic characterization of immunoaffinity interactions is presented.

• MeSH
• Equipment Failure Analysis MeSH
• Biosensing Techniques methods   instrumentation   MeSH
• Equipment Design MeSH
• Immunoassay instrumentation   MeSH
• Protein Interaction Mapping methods   instrumentation   MeSH
• Transducers MeSH
• Micro-Electrical-Mechanical Systems instrumentation   MeSH
• Flow Injection Analysis instrumentation   MeSH
• Reproducibility of Results MeSH
• Sensitivity and Specificity MeSH