The purpose of our study was to examine an early activation of JNK and p38 mitogen activated protein kinases (MAPK) and their substrate c-Myc after soman poisoning in order to enlighten the pathogenetic mechanism of nerve agent-induced non-specific effects. Male Wistar rats were intramuscularly poisoned by soman (60 μg.kg-1 - 70% LD50). Samples were taken 4, 24, and 72 hours after poisoning, immunohistochemically stained and phospho-JNKThr-183/Tyr-185, phospho-p38Thr180/Tyr182, and phospho-c- MycThr58/Ser62 expressions were measured using a computer Image analysis in apical and cryptal enterocytes of the colon transversum. We observed decreased phospho-JNK in apical enterocytes 4 and 24 h after poisoning and increased phospho-JNK in cryptal and apical enterocytes 72 h after intoxication. Phosphop38 dropped significantly in the apical compartment 72 h after soman poisoning. An activation of c-Myc decreased in both apical and cryptal compartment 4 and 24 h after soman intoxication, while increased in both compartments 72 h after poisoning. Soman poisoning seems to temporarily suppress promitotic pathways of proliferating cryptal cells and causes delayed activation of JNK stress signaling pathway.
- MeSH
- časové faktory MeSH
- colon transversum chemie MeSH
- enterocyty chemie MeSH
- experimenty na zvířatech MeSH
- financování organizované MeSH
- JNK mitogenem aktivované proteinkinasy chemie MeSH
- kontrolní skupiny MeSH
- MAP kinasový signální systém fyziologie MeSH
- mitogenem aktivované proteinkinasy p38 chemie MeSH
- potkani Wistar MeSH
- protoonkogenní proteiny c-myc chemie MeSH
- soman otrava toxicita MeSH
- výzkumný projekt MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- MeSH
- destičkový růstový faktor chemie MeSH
- exprese genu MeSH
- fibroblastové růstové faktory chemie MeSH
- finanční podpora výzkumu jako téma MeSH
- imunohistochemie MeSH
- lidé MeSH
- melanom diagnóza patologie MeSH
- névus diagnóza patologie MeSH
- protoonkogenní proteiny c-myc chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
