This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method. We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test. The results showed that all types of scaffolds should be safe for use in the human body. The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp. This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold. Based on our results we concluded that the best PVA/HA/HAp combination is in the ratio 3:1:2.
- MeSH
- biokompatibilní materiály metabolismus farmakologie MeSH
- buněčná adheze účinky léků MeSH
- hydrogely chemie MeSH
- hydroxyapatit metabolismus farmakologie MeSH
- kyselina hyaluronová metabolismus farmakologie MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- osteoblasty metabolismus MeSH
- polyvinylalkohol metabolismus farmakologie MeSH
- proliferace buněk účinky léků MeSH
- testování materiálů metody MeSH
- tkáňové inženýrství metody MeSH
- tkáňové podpůrné struktury chemie MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The formation of mineralized tissues is governed by extracellular matrix proteins that assemble into a 3D organic matrix directing the deposition of hydroxyapatite. Although the formation of bones and dentin depends on the self-assembly of type I collagen via the Gly-X-Y motif, the molecular mechanism by which enamel matrix proteins (EMPs) assemble into the organic matrix remains poorly understood. Here we identified a Y/F-x-x-Y/L/F-x-Y/F motif, evolutionarily conserved from the first tetrapods to man, that is crucial for higher order structure self-assembly of the key intrinsically disordered EMPs, ameloblastin and amelogenin. Using targeted mutations in mice and high-resolution imaging, we show that impairment of ameloblastin self-assembly causes disorganization of the enamel organic matrix and yields enamel with disordered hydroxyapatite crystallites. These findings define a paradigm for the molecular mechanism by which the EMPs self-assemble into supramolecular structures and demonstrate that this process is crucial for organization of the organic matrix and formation of properly structured enamel.
- MeSH
- amelogenin metabolismus MeSH
- aminokyselinové motivy fyziologie MeSH
- biologická evoluce MeSH
- extracelulární matrix - proteiny metabolismus MeSH
- hydroxyapatit metabolismus MeSH
- myši MeSH
- proteiny zubní skloviny metabolismus MeSH
- sekvence aminokyselin MeSH
- vazba proteinů fyziologie MeSH
- vnitřně neuspořádané proteiny metabolismus MeSH
- zubní sklovina metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
The remediation of Pb(II) through biomineralization is rergarded as a promising technique as well as an interesting phenomenon for transforming heavy metals from mobile species into very stable minerals in the environment. Studies are well needed for in-depth understanding the mechanism of Pb(II) immobilized by bacteria. In the present study, we investigated the uptake and biomineralization of Pb(II) using Bacillus cereus 12-2 isolated from lead-zinc mine tailings. The maximum Pb(II) uptake capacity of B. cereus 12-2 was 340 mg/g at pH 3.0. Zeta potential analyses and selective passivation experiments demonstrated that electrostatic attraction was the main force driving the uptake of Pb(II), while the carboxyl, amide and phosphate functional groups of the bacteria provided the binding sites for immobilizing Pb(II). XRD and TEM investigation revealed that the Pb(II) loaded on bacteria could be stepwise transformed into rod-shaped Ca2.5Pb7.5(OH)2(PO4)6 nanocrystal. Combined with protein denaturalization experiments, we proposed that the biomineralization of Pb(II) possibly consisted of two steps: (1) Rapid biosorption of Pb(II) on B. cereus 12-2 through the synergy of electrostatic attraction, ionic exchange and chelating activity of functional groups; (2) enzyme-mediated mineral transformation from amorphous precipitate to rod-shaped crystalline minerals happening gradually inside the bacteria.
- MeSH
- dietoterapie metody trendy využití MeSH
- experimenty na zvířatech MeSH
- fosfor * izolace a purifikace metabolismus nedostatek MeSH
- hydroxyapatit izolace a purifikace metabolismus MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- metabolické nemoci diagnóza etiologie terapie MeSH
- mléko metabolismus využití MeSH
- osteoporóza diagnóza metabolismus prevence a kontrola MeSH
- poruchy metabolismu vápníku * diagnóza etiologie MeSH
- statistika jako téma MeSH
- vápník * izolace a purifikace metabolismus nedostatek MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
