Interleukin-10 prevents transition of a physiological inflammatory reaction to a pathological state that may result in neuropathic pain. We studied bilateral changes of IL-10 protein levels in L4-L5 and C7-C8 dorsal root ganglia (DRG) after a chronic constriction injury (CCI) of either L4-L5 spinal nerves (pCCI) or the sciatic nerve (dCCI). Rats undergoing pCCI or dCCI were left to survive for 1, 3, 7 or 14 d, sham-operated rats for 3 or 14 d. After the survival time, C7-C8 and L4-L5 DRG were removed bilaterally from naïve, operated, and sham-operated rats and IL-10 protein was detected by immunohistochemical staining and measured using ELISA analysis. Unilateral pCCI and dCCI induced a transient bilateral elevation in IL-10 protein level not only in the homonymous lumbar DRG but also in the heteronymous cervical DRG nonassociated with the spinal segments of constricted nerve. Sham operations also induced bilateral elevation of IL-10 protein in both homonymous and heteronymous DRG. Our experiments revealed that the more proximal is a nerve injury the more rapid is the initial increase and slower the subsequent decrease of IL-10 protein level in DRG. Changes of IL-10 protein in DRG nonassociated with damaged nerve could be related to a general neuroinflammatory reaction of the nervous system to injury and thereby promote potential of the DRG neurons for regenerating their axons following a conditioning lesion.
- MeSH
- axony patologie MeSH
- chronická nemoc MeSH
- interleukin-10 metabolismus MeSH
- krysa rodu rattus MeSH
- míšní nervy metabolismus patologie MeSH
- modely nemocí na zvířatech MeSH
- nemoci periferního nervového systému metabolismus patologie patofyziologie MeSH
- nemoci sedacího nervu metabolismus patologie patofyziologie MeSH
- nervus ischiadicus metabolismus patologie MeSH
- potkani Wistar MeSH
- spinální ganglia metabolismus patologie patofyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Cíl studie: Informace o neurologických komplikacích gynekologických pánevních operací. Typ studie: Literární přehled a kazuistika. Název a sídlo pracoviště: Gynekologicko-porodnická klinika LF UK a FN, Plzeň. Metodika: Literární přehled o poruchách cerebrospinálních nervů v oblasti plexus lumbalis a plexus sacralis při gynekologických pánevních operacích s ilustrativní kazuistikou. Závěr: Neurologické komplikace pánevních operací jsou často zpočátku málo zřetelné, a tak jsou relativně pozdě diagnostikovány. Při jejich řešení je potřebná spolupráce s neurology a anesteziology.
Objective: To summarize literary data dealing with neurological complications during gynecological pelvic surgery and present a case report. Design: Literary review and a case report. Setting: Department of Obstetrics and Gynecology, Medical Faculty Hospital Plzeň. Methods: Literary review of lesions of cerebrospinal nerves during gynecological pelvic surgery, case report. Conclusion: Neurological complications of pelvic surgery are usually discreet and their diagnosis is often delayed. A close cooperation with neurologists and anestesiologists is necessary.
- MeSH
- míšní nervy patologie MeSH
- mononeuropatie MeSH
- muskuloskeletální systém inervace patofyziologie MeSH
- Publikační typ
- recenze MeSH
