Chronic pain is associated with time-dependent structural and functional reorganization of the prefrontal cortex that may reflect adaptive pain compensatory and/or maladaptive pain-promoting mechanisms. However, the molecular underpinnings of these changes and whether there are time-dependent relationships to pain progression are not well characterized. In this study, we analyzed protein composition in the medial prefrontal cortex (mPFC) of rats at two timepoints after spinal nerve ligation (SNL) using two-dimensional gel electrophoresis (2D-ELFO) and liquid chromatography with tandem mass spectrometry (LC-MS/MS). SNL, but not sham-operated, rats developed persistent tactile allodynia and thermal hyperalgesia, confirming the presence of experimental neuropathic pain. Two weeks after SNL (early timepoint), we identified 11 proteins involved in signal transduction, protein transport, cell homeostasis, metabolism, and apoptosis, as well as heat-shock proteins and chaperones that were upregulated by more than 1.5-fold compared to the sham-operated rats. Interestingly, there were only four significantly altered proteins identified at 8 weeks after SNL (late timepoint). These findings demonstrate extensive time-dependent modifications of protein expression in the rat mPFC under a chronic neuropathic pain state that might underlie the evolution of chronic pain characterized by early pain-compensatory and later aberrant mechanisms.
- MeSH
- časové faktory MeSH
- chromatografie kapalinová MeSH
- hyperalgezie etiologie metabolismus MeSH
- krysa rodu rattus MeSH
- měření bolesti MeSH
- míšní nervy zranění MeSH
- neuralgie etiologie metabolismus MeSH
- potkani Sprague-Dawley MeSH
- prefrontální mozková kůra metabolismus MeSH
- proteomika metody MeSH
- regulace genové exprese MeSH
- tandemová hmotnostní spektrometrie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Cíl studie: Informace o neurologických komplikacích gynekologických pánevních operací. Typ studie: Literární přehled a kazuistika. Název a sídlo pracoviště: Gynekologicko-porodnická klinika LF UK a FN, Plzeň. Metodika: Literární přehled o poruchách cerebrospinálních nervů v oblasti plexus lumbalis a plexus sacralis při gynekologických pánevních operacích s ilustrativní kazuistikou. Závěr: Neurologické komplikace pánevních operací jsou často zpočátku málo zřetelné, a tak jsou relativně pozdě diagnostikovány. Při jejich řešení je potřebná spolupráce s neurology a anesteziology.
Objective: To summarize literary data dealing with neurological complications during gynecological pelvic surgery and present a case report. Design: Literary review and a case report. Setting: Department of Obstetrics and Gynecology, Medical Faculty Hospital Plzeň. Methods: Literary review of lesions of cerebrospinal nerves during gynecological pelvic surgery, case report. Conclusion: Neurological complications of pelvic surgery are usually discreet and their diagnosis is often delayed. A close cooperation with neurologists and anestesiologists is necessary.
