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MeSH: melanom-chemie

10 záznamů v Medvik Filtry

Článek

Rapid SERS-based recognition of cell secretome on the folic acid-functionalized gold gratings

Guselnikova, Olga
Autor Guselnikova, Olga Department of Solid State Engineering, University of Chemistry and Technology, 166 28, Prague, Czech Republic. Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk, Russian Federation, 634040
Dvorankova, Barbara
Autor Dvorankova, Barbara Institute of Anatomy, 1st Faculty of Medicine, Charles University, 166 36, Prague, Czech Republic
Kakisheva, Kamila
Autor Kakisheva, Kamila Department of Solid State Engineering, University of Chemistry and Technology, 166 28, Prague, Czech Republic
Kalachyova, Yevgeniya
Autor Kalachyova, Yevgeniya Department of Solid State Engineering, University of Chemistry and Technology, 166 28, Prague, Czech Republic. Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk, Russian Federation, 634040
Postnikov, Pavel
Autor Postnikov, Pavel Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk, Russian Federation, 634040
Svorcik, Vaclav
Autor Svorcik, Vaclav Department of Solid State Engineering, University of Chemistry and Technology, 166 28, Prague, Czech Republic
Lyutakov, Oleksiy
Autor Lyutakov, Oleksiy Department of Solid State Engineering, University of Chemistry and Technology, 166 28, Prague, Czech Republic. lyutakoo@vscht.cz. Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk, Russian Federation, 634040. lyutakoo@vscht.cz

Analytical and bioanalytical chemistry. 2019 ; 411 (15) : 3309-3319. [pub] 20190523

Anal Bioanal Chem
ISSN 1618-2650
Medvik
Zdroj

Nowadays, functionalization of the plasmon-supported nanostructured surface is considered as a powerful tool for tumour cell recognition. In this study, the SERS on a surface plasmon polariton-supported gold grating functionalized with folic acid was used to demonstrate an unpretentious recognition of melanoma-associated fibroblasts. Using cultivation media conditioned by different cells, we were able to detect reproducible differences in the secretome of melanoma-associated and normal control fibroblasts. The homogeneous distribution of plasmon energy along the grating surface was proved to provide excellent SERS signal reproducibility, while, to increase the affinity of (bio)molecules to SERS substrate, folic acid molecules were covalently grafted to the gold gratings. As proof of concept, fibroblasts were cultured in vitro, and culture media from the normal and tumour-associated lines were collected and analysed with our proposed SERS substrates. Identifying individual peaks of the Raman spectra as well as comparing their relative intensities, we showed that the proposed functional SERS platform can recognise the melanoma-associated cells without the need for further statistical spectral evaluation directly. We also demonstrated that the SERS chip created provided a stable SERS signal over a period of 90 days without loss of sensitivity. Graphical abstract.