CONCLUSION: Our results encourage the notion that the pharynx could be an extragastric reservoir of Helicobacter pylori (HP). The study confirmed the presence of HP in adenotonsillar tissue in children. It could have importance in the pathophysiology of upper respiratory diseases. However, its precise role in these processes remains unclear and requires further studies. OBJECTIVE: A prospective study was carried out to evaluate the presence of HP in tonsillar and adenoid tissue in children. The study focused on real-time PCR analyzing CagA and VacA genotypes of HP strains. METHODS: A total of 37 consecutive pediatric patients with adenotonsillar hypertrophy indicated for surgery were observed in a prospective study. Adenoidectomy and/or tonsillectomy was performed in each patient; 49 specimens were taken, 32 from adenoids and 17 from tonsils. The presence of HP and its genotype were tested in all samples by real-time PCR analysis. RESULTS: Of 49 samples analyzed, 48 were positive for the presence of HP (98%), so only 1 sample was negative. While the genotype VacAs1bm2 was definitely dominant in adenoid tissue, wider distribution was observed in tonsillar tissue. Cag(+) strains represented one-fifth of all samples (21%).
- MeSH
- adenoidy mikrobiologie patologie MeSH
- antigeny bakteriální analýza MeSH
- bakteriální proteiny analýza MeSH
- dítě MeSH
- Helicobacter pylori izolace a purifikace MeSH
- hypertrofie mikrobiologie MeSH
- infekce vyvolané Helicobacter pylori epidemiologie MeSH
- krční mandle mikrobiologie patologie MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé MeSH
- nemoci faryngu mikrobiologie MeSH
- předškolní dítě MeSH
- prevalence MeSH
- prospektivní studie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
CONCLUSION: Our results encourage the notion that the pharynx could be an extragastric reservoir of Helicobacter pylori (HP). The study confirmed the presence of HP in adenotonsillar tissue in children. It could have importance in the pathophysiology of upper respiratory diseases. However, its precise role in these processes remains unclear and requires further studies. OBJECTIVE: A prospective study was carried out to evaluate the presence of HP in tonsillar and adenoid tissue in children. The study focused on real-time PCR analyzing CagA and VacA genotypes of HP strains. METHODS: A total of 37 consecutive pediatric patients with adenotonsillar hypertrophy indicated for surgery were observed in a prospective study. Adenoidectomy and/or tonsillectomy was performed in each patient; 49 specimens were taken, 32 from adenoids and 17 from tonsils. The presence of HP and its genotype were tested in all samples by real-time PCR analysis. RESULTS: Of 49 samples analyzed, 48 were positive for the presence of HP (98%), so only 1 sample was negative. While the genotype VacAs1bm2 was definitely dominant in adenoid tissue, wider distribution was observed in tonsillar tissue. Cag(+) strains represented one-fifth of all samples (21%).
- MeSH
- adenoidy * mikrobiologie patologie MeSH
- antigeny bakteriální analýza MeSH
- bakteriální proteiny analýza MeSH
- dítě MeSH
- Helicobacter pylori * izolace a purifikace MeSH
- infekce vyvolané Helicobacter pylori epidemiologie MeSH
- krční mandle * mikrobiologie patologie MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- nemoci faryngu * mikrobiologie MeSH
- předškolní dítě MeSH
- prevalence MeSH
- prospektivní studie MeSH
- Check Tag
- dítě MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Geografické názvy
- Česká republika MeSH
- Klíčová slova
- DIFLUCAN, SPORANOX,
- MeSH
- antifungální látky aplikace a dávkování MeSH
- karcinom komplikace MeSH
- lidé MeSH
- nemoci faryngu mikrobiologie MeSH
- orální kandidóza farmakoterapie MeSH
- Check Tag
- lidé MeSH
