Význam gramnegatívnej baktérie Stenotrophomonas maltopbilia, spôsobujúcej infekcie u imunodeficientních pacientov, v poslednej dobe vzrastá hlavne kvôli prirodzenej rezistencii voči mnohým antimikróbnym látkam. Cieľom štúdie bolo zistiť postantibiotický účinok (PAE) vybraných aminoglykozidov a charakterizovať jeho pôsobenie na niektoré biologické vlastnosti klinických izolátor S. maltophilia. PAE sa určil použitím suprainhibičných koncentrácií 2x a 4x minimálna inhibičná koncentrácia (MIC) amikacínu, netilmicínu a tobramycínu pri 37 °C 24 h v Mulerovom-Hintonovom bujóne (MHB). Metódou adherencie na xylén a soľnoagregačným testom sa stanovila povrchová hydrofobicita buniek S. malíophilia, dalei sa hodnotila motilita a produkcia lipázy u testovaných kmeňov. Najdlhší PAE – priemerne 2 h indukoval netilmicín u kmeňa č. 5 777. Aminoglykozidy v suprainhibičných koncentráciách neovplyvnili výraznejšie povrchovú hydrofobicitu ani motilitu u testovaných kmeňov. Netilmicín najvýraznejšie inhiboval produkciu lipázy v koncentrácii 4x MIC u kmeňa č. 27 823. Zistené účinky suprainhibičných koncentrácií aminoglykozidov na S. ma/tophiiia boli všeobecne nižšie jako při iných gramnegatívnych baktériách.
Recently, the importance of a gramnegative bacterium Stenotrophomonas maltophilia, causing infection in immunocompromised patients, grows rapidly, mainly because of its primary resistance to many antibiotics. The aim of the study was to characterize a postantibiotic effect (PAE) of chosen aminoglycosides and their influence on some biological properties of clinical isolates of S. maltophilia. The PAE was detected using suprainhibitory concentrations of 2x and 4x minimal inhibitory concentration (MIC) of amikacin, netilmicin and tobramycin at 37 °C in Miiller-Hinton broth for 24 h. The surface hydrophobicity of S. maltophilia cells was determined by adherence to xylen and salt aggregation test. Further, motility and production of lipase in strains tested was evaluated as well. The longest PAE - on the average 2 h has been induced by netilmicin on the strain No. 5 777. The infuence of suprainhibitory concentrations of aminoglycosides tested on the surface hydrophobicity and motility of both strains was not very apparend. The production of lipase was inhibited by netilimcin at 4x MIC level at the strain No. 27 823. The effects of suprainhibitory concentrations of aminoglycosides on S. maltophilia were generally lower in comparison to other gramnegative bacteria.
- MeSH
- Medical Oncology MeSH
- Humans MeSH
- Netilmicin administration & dosage blood MeSH
- Drug-Related Side Effects and Adverse Reactions MeSH
- Patients MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Comparative Study MeSH
- MeSH
- Leukemia, Myeloid, Acute complications MeSH
- Adult MeSH
- Fever drug therapy MeSH
- Carcinoma complications MeSH
- Drug Therapy, Combination MeSH
- Humans MeSH
- Netilmicin administration & dosage pharmacology MeSH
- Neutropenia drug therapy MeSH
- Pefloxacin administration & dosage therapeutic use MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Comparative Study MeSH
- MeSH
- Agranulocytosis complications MeSH
- Amikacin administration & dosage therapeutic use MeSH
- Ceftriaxone administration & dosage therapeutic use MeSH
- Adult MeSH
- Fever drug therapy MeSH
- Middle Aged MeSH
- Humans MeSH
- Neoplasms complications MeSH
- Netilmicin administration & dosage therapeutic use MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
