The antibacterial effects of essential oils and their components (EOCs) are usually attributed to effects on membranes and metabolism. Studies of the effects of EOCs on protein expression have primarily analysed proteins larger than 10 kDa using gel electrophoresis. In the present study, we used MALDI-TOF-MS to investigate the effects of EOCs on low-molecular-weight proteins. From 297 m/z features, we identified 94 proteins with important differences in expression among untreated samples, samples treated with EOCs, and samples treated with antibiotics, peroxide, or chlorine. The targets of these treatments obviously differ, even among EOCs. In addition to ribosomal proteins, stress-, membrane- and biofilm-related proteins were affected. These findings may provide a basis for identifying new targets of essential oils and synergies with other antibiotics.
- MeSH
- antibakteriální látky metabolismus MeSH
- Escherichia coli účinky léků fyziologie MeSH
- fyziologický stres * MeSH
- molekulová hmotnost MeSH
- oleje prchavé metabolismus MeSH
- proteiny z Escherichia coli analýza chemie MeSH
- proteom analýza MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Recently, a growing number of plant essential oils (EOs) have been tested against a wide range of arthropod pests with promising results. EOs showed high effectiveness, multiple mechanisms of action, low toxicity on non-target vertebrates and potential for the use of byproducts as reducing and stabilizing agents for the synthesis of nanopesticides. However, the number of commercial biopesticides based on EOs remains low. We analyze the main strengths and weaknesses arising from the use of EO-based biopesticides. Key challenges for future research include: (i) development of efficient stabilization processes (e.g., microencapsulation); (ii) simplification of the complex and costly biopesticide authorization requirements; and (iii) optimization of plant growing conditions and extraction processes leading to EOs of homogeneous chemical composition.
Research on the role of arbuscular mycorrhizal fungi (AMF) in the synthesis of essential oils (EOs) by aromatic plants has seldom been conducted in field-relevant conditions, and then, only limited spectra of EO constituents have been analyzed. The effect was investigated of inoculation with AMF on the synthesis of a wide range of EO in two aromatic species, coriander (Coriandrum sativum) and dill (Anethum graveolens), in a garden experiment under outdoor conditions. Plants were grown in 4-l pots filled with soil, which was either γ-irradiated (eliminating native AMF) or left non-sterile (containing native AMF), and inoculated or not with an isolate of Rhizophagus irregularis. AMF inoculation significantly stimulated EO synthesis in both plant species. EO synthesis (total EO and several individual constituents) was increased in dill in all mycorrhizal treatments (containing native and/or inoculated AMF) compared to non-mycorrhizal plants. In contrast, EO concentrations in coriander (total EO and most constituents) were increased only in the treatment combining both inoculated and native AMF. A clear positive effect of AMF on EO synthesis was found for both aromatic plants, which was, however, specific for each plant species and modified by the pool of AMF present in the soil.
- MeSH
- bazalka fyziologie chemie účinky léků MeSH
- cholesterol izolace a purifikace krev MeSH
- experimenty na zvířatech statistika a číselné údaje MeSH
- feces chemie MeSH
- financování organizované MeSH
- hyperlipidemie etiologie farmakoterapie metabolismus MeSH
- hypolipidemika aplikace a dávkování chemie metabolismus MeSH
- játra chemie účinky léků MeSH
- krevní glukóza izolace a purifikace MeSH
- lipidy chemie izolace a purifikace MeSH
- oleje prchavé aplikace a dávkování chemie metabolismus MeSH
- oleje rostlin farmakologie chemie terapeutické užití MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí metody přístrojové vybavení využití MeSH
- potkani Wistar metabolismus MeSH
- rostlinné extrakty farmakologie chemie terapeutické užití MeSH
- statistika jako téma metody MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
