Knoppova, Lenka*
Dotaz
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- MeSH
- dítě MeSH
- laparoskopie * MeSH
- lidé MeSH
- nedepolarizující myorelaxancia * MeSH
- nervosvalová blokáda * škodlivé účinky MeSH
- pilotní projekty MeSH
- rokuronium MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- dopisy MeSH
- randomizované kontrolované studie MeSH
This study aimed to test the antiproliferative effect of three benzimidazole anthelmintics in intestinal cancer cells and to investigate whether these drugs, which inhibit tubulin polymerization, can potentiate the efficacy of the microtubule-stabilizing drug paclitaxel (PTX). Four intestinal cancer cell lines, SW480, SW620, HCT8, and Caco2, with different origins and growth characteristics were used. The antiproliferative effect of albendazole (ABZ), ricobendazole (RBZ), flubendazole (FLU), and their combinations with PTX was tested using three different end-point viability assays, cell cycle distribution analysis, and the x-CELLigence System for real-time cell analysis. ABZ and FLU inhibited cell proliferation significantly in a concentration-dependent and time-dependent manner through cell arrest in the G2/M phase. RBZ was not effective at any concentration tested. The cell lines differed in sensitivity to FLU and ABZ, with HCT8 being the most sensitive, showing IC₅₀ values for ABZ and FLU that reached 0.3 and 0.9 μmol/l, respectively. Combinations of PTX+ABZ and PTX+FLU decreased cell viability more effectively when compared with treatment with individual drugs alone. The anthelmintic benzimidazole drugs ABZ and FLU show a significant cytostatic effect and potentiate the efficacy of PTX in intestinal cancer cells.
- MeSH
- adenokarcinom farmakoterapie MeSH
- albendazol analogy a deriváty farmakologie MeSH
- anthelmintika farmakologie MeSH
- chemorezistence účinky léků MeSH
- fytogenní protinádorové látky farmakologie MeSH
- G2 fáze účinky léků MeSH
- inhibiční koncentrace 50 MeSH
- lidé MeSH
- mebendazol analogy a deriváty farmakologie MeSH
- mikrotubuly účinky léků MeSH
- modulátory tubulinu farmakologie MeSH
- nádorové buněčné linie MeSH
- paclitaxel farmakologie MeSH
- proliferace buněk účinky léků MeSH
- protinádorové látky farmakologie MeSH
- stabilita proteinů účinky léků MeSH
- střevní nádory farmakoterapie MeSH
- synergismus léků MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
Photosystem II (PSII) is a large enzyme complex embedded in the thylakoid membrane of oxygenic phototrophs. The biogenesis of PSII requires the assembly of more than 30 subunits, with the assistance of a number of auxiliary proteins. In plants and cyanobacteria, the photosynthesis-affected mutant 68 (Pam68) is important for PSII assembly. However, its mechanisms of action remain unknown. Using a Synechocystis PCC 6803 strain expressing Flag-tagged Pam68, we purified a large protein complex containing ribosomes, SecY translocase, and the chlorophyll-binding PSII inner antenna CP47. Using 2D gel electrophoresis, we identified a pigmented Pam68-CP47 subcomplex and found Pam68 bound to ribosomes. Our results show that Pam68 binds to ribosomes even in the absence of CP47 translation. Furthermore, Pam68 associates with CP47 at an early phase of its biogenesis and promotes the synthesis of this chlorophyll-binding polypeptide until the attachment of the small PSII subunit PsbH. Deletion of both Pam68 and PsbH nearly abolishes the synthesis of CP47, which can be restored by enhancing chlorophyll biosynthesis. These results strongly suggest that ribosome-bound Pam68 stabilizes membrane segments of CP47 and facilitates the insertion of chlorophyll molecules into the translated CP47 polypeptide chain.
- MeSH
- 2D gelová elektroforéza MeSH
- bakteriální proteiny genetika metabolismus MeSH
- buněčná membrána metabolismus MeSH
- chlorofyl metabolismus MeSH
- fosfoproteiny genetika metabolismus MeSH
- fotosystém II (proteinový komplex) genetika metabolismus MeSH
- mutace MeSH
- ribozomy metabolismus MeSH
- světlosběrné proteinové komplexy genetika metabolismus MeSH
- Synechocystis genetika metabolismus MeSH
- vazba proteinů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Ergot alkaloids are widely used in the pharmaceutical industry in drug preparations for treating migraines and Parkinson's disease, inducing uterine contraction, and other purposes. Phytopathogenic fungi of the genus Claviceps (e.g. C. purpurea) comprise a major biological source of ergot alkaloids. Worldwide industrial production of these alkaloids derives almost equally from two biotechnological procedures: submerged culture of the fungus in fermenters and field parasitic production in dormant fungal organs known as sclerotia (also termed ergot). Ergot yields from field cultivation are greatly affected by weather and also can be much reduced by pollen contamination from imperfectly male-sterile rye, as only unfertilized ovaries can be infected by C. purpurea spores. Two substances with gametocidal effect - maleic hydrazide and 2-chloroethylphosphonic acid - were tested during three consecutive seasons in small field experiments for the ability to induce or amplify the male sterility of rye as well as the impacts on germination of C. purpurea spores and general vitality of rye host plants. Maleic hydrazide was proven to be a highly effective gametocide on both a fertile rye variety and a variety with imperfectly induced cytoplasmic male sterility. It showed negligible effect on germination of C. purpurea spores. Both accurate dosaging of the active gametocidal compound and timing of the application just 2-3 weeks before onset of anthesis proved crucial to achieving high ergot yield with minimum grain impurities.
- MeSH
- hydrazid kyseliny maleinové aplikace a dávkování MeSH
- námelové alkaloidy biosyntéza izolace a purifikace MeSH
- neplodnost rostlin účinky léků fyziologie MeSH
- organofosforové sloučeniny aplikace a dávkování MeSH
- regulátory růstu rostlin farmakologie MeSH
- upregulace účinky léků fyziologie MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zárodečné buňky rostlin účinky léků MeSH
- žito účinky léků metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
... Lenko Knoppová) -- 7.1 Dokumentace na operačním sále 114 -- 7.2 Dokumentace na lůžkovém anesteziologicko-resuscitačním ...
Vyd. 1. 268 s. : il., tab. ; 21 cm
- MeSH
- anesteziologie MeSH
- dítě MeSH
- Check Tag
- dítě MeSH
- Publikační typ
- monografie MeSH
- Konspekt
- Pediatrie
- NLK Obory
- anesteziologie a intenzivní lékařství
- pediatrie
- NLK Publikační typ
- učebnice vysokých škol
1223 stran : barevné ilustrace ; 26 cm
1240 stran bohatě obrazově dokumentované barevné publikace je rozděleno do padesáti kapitol. Kniha je již tradiční učebnicí a biblí všech anesteziologů - začínajících i zkušených, její aktualizovaná vydání vycházejí již přes 30 let.
- MeSH
- anesteziologie MeSH
- Konspekt
- Ortopedie. Chirurgie. Oftalmologie
- NLK Obory
- anesteziologie a intenzivní lékařství
