Cardiovascular lineages develop together with kidney, smooth muscle, and limb connective tissue progenitors from the lateral plate mesoderm (LPM). How the LPM initially emerges and how its downstream fates are molecularly interconnected remain unknown. Here, we isolate a pan-LPM enhancer in the zebrafish-specific draculin (drl) gene that provides specific LPM reporter activity from early gastrulation. In toto live imaging and lineage tracing of drl-based reporters captures the dynamic LPM emergence as lineage-restricted mesendoderm field. The drl pan-LPM enhancer responds to the transcription factors EomesoderminA, FoxH1, and MixL1 that combined with Smad activity drive LPM emergence. We uncover specific activity of zebrafish-derived drl reporters in LPM-corresponding territories of several chordates including chicken, axolotl, lamprey, Ciona, and amphioxus, revealing a universal upstream LPM program. Altogether, our work provides a mechanistic framework for LPM emergence as defined progenitor field, possibly representing an ancient mesodermal cell state that predates the primordial vertebrate embryo.

• MeSH
• dánio pruhované MeSH
• embryo nesavčí MeSH
• embryonální indukce genetika   MeSH
• gastrulace genetika   MeSH
• intravitální mikroskopie MeSH
• mezoderm embryologie   MeSH
• proteiny dánia pruhovaného genetika   MeSH
• vývojová regulace genové exprese * MeSH
• zesilovače transkripce * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• audiovizuální média MeSH
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH

• MeSH
• ektoderm účinky léků   MeSH
• embryonální indukce MeSH
• fetální proteiny izolace a purifikace   MeSH
• králíci MeSH
• mezoderm fyziologie   MeSH
• oligosacharidy imunologie   metabolismus   MeSH
• proteoglykany imunologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• zvířata MeSH

• MeSH
• ektoderm anatomie a histologie   MeSH
• končetiny embryologie   MeSH
• kuřecí embryo * MeSH
• mezoderm anatomie a histologie   MeSH
• zvířata MeSH
• Check Tag
• kuřecí embryo * MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• chorda dorsalis MeSH
• DNA vazebné proteiny genetika   MeSH
• fylogeneze MeSH
• homeodoménové proteiny fyziologie   chemie   MeSH
• mezoderm fyziologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH

Fibroblasts, the most abundant cell type in the human body, play crucial roles in biological processes such as inflammation and cancer progression. They originate from the mesoderm or neural-crest-derived ectomesenchyme. Ectomesenchyme-derived fibroblasts contribute to facial formation and do not express HOX genes during development. The expression and role of the HOX genes in adult fibroblasts is not known. We investigated whether the developmental pattern persists into adulthood and under pathological conditions, such as cancer. We collected adult fibroblasts of ectomesenchymal and mesodermal origins from distinct body parts. The isolated fibroblasts were characterised by immunocytochemistry, and their transcriptome was analysed by whole genome profiling. Significant differences were observed between normal fibroblasts from the face (ectomesenchyme) and upper limb (mesoderm), particularly in genes associated with limb development, including HOX genes, e.g., HOXA9 and HOXD9. Notably, the pattern of HOX gene expression remained consistent postnatally, even in fibroblasts from pathological tissues, including inflammatory states and cancer-associated fibroblasts from primary and metastatic tumours. Therefore, the distinctive HOX gene expression pattern can serve as an indicator of the topological origin of fibroblasts. The influence of cell position and HOX gene expression in fibroblasts on disease progression warrants further investigation.

• MeSH
• dospělí MeSH
• fibroblasty * metabolismus   cytologie   MeSH
• homeoboxové geny MeSH
• homeodoménové proteiny metabolismus   genetika   MeSH
• kultivované buňky MeSH
• lidé MeSH
• mezoderm * metabolismus   cytologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

The tight regulation of cytoskeleton dynamics is required for a number of cellular processes, including migration, division and differentiation. YAP-TEAD respond to cell-cell interaction and to substrate mechanics and, among their downstream effects, prompt focal adhesion (FA) gene transcription, thus contributing to FA-cytoskeleton stability. This activity is key to the definition of adult cell mechanical properties and function. Its regulation and role in pluripotent stem cells are poorly understood. Human PSCs display a sustained basal YAP-driven transcriptional activity despite they grow in very dense colonies, indicating these cells are insensitive to contact inhibition. PSC inability to perceive cell-cell interactions can be restored by tampering with Tankyrase enzyme, thus favouring AMOT inhibition of YAP function. YAP-TEAD complex is promptly inactivated when germ layers are specified, and this event is needed to adjust PSC mechanical properties in response to physiological substrate stiffness. By providing evidence that YAP-TEAD1 complex targets key genes encoding for proteins involved in cytoskeleton dynamics, we suggest that substrate mechanics can direct PSC specification by influencing cytoskeleton arrangement and intracellular tension. We propose an aberrant activation of YAP-TEAD1 axis alters PSC potency by inhibiting cytoskeleton dynamics, thus paralyzing the changes in shape requested for the acquisition of the given phenotype.

• MeSH
• adaptorové proteiny signální transdukční MeSH
• angiomotiny metabolismus   MeSH
• buněčná diferenciace MeSH
• buněčné linie MeSH
• cytoskelet metabolismus   MeSH
• lidé MeSH
• lidské embryonální kmenové buňky metabolismus   MeSH
• mezoderm metabolismus   MeSH
• signální proteiny YAP genetika   metabolismus   MeSH
• signální transdukce MeSH
• transkripční faktory TEA domény genetika   metabolismus   MeSH
• vazba proteinů MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• MeSH
• dítě MeSH
• kojenec MeSH
• lidé MeSH
• mezoderm embryologie   patofyziologie   MeSH
• novorozenec MeSH
• pánev genetika   patologie   MeSH
• skolióza genetika   MeSH
• urogenitální abnormality MeSH
• Check Tag
• dítě MeSH
• kojenec MeSH
• lidé MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

• MeSH
• Chordata MeSH
• embryonální vývoj MeSH
• epigeneze genetická MeSH
• fylogeneze MeSH
• hlava embryologie   MeSH
• mezoderm MeSH
• somity MeSH
• Publikační typ
• monografie MeSH

• Konspekt
• Biologické vědy
• NLK Obory
• biologie
• biologie

• MeSH
• discus nervi optici anatomie a histologie   MeSH
• mezoderm MeSH

• MeSH
• embryologie MeSH
• hlava embryologie   MeSH
• mezoderm MeSH
• obratlovci MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH