Gastric dysmotility can be a sign of common diseases such as longstanding diabetes mellitus. It is known that the application of high-frequency low-energetic stimulation can help to effectively moderate and alleviate the symptoms of gastric dysmotility. The goal of our research was the development of a miniature, endoscopically implantable device to a submucosal pocket. The implantable device is a fully customized electronics package which was specifically designed for the purpose of experiments in the submucosa. The device was endoscopically inserted into the submucosal pocket of a pig stomach and partially severed pig side in order to adequately simulate a live animal model. The experiment confirmed that the designed device can be implanted into the submucosa and is capable of the measurement of sensor data and the transmission of this data wirelessly in real time to a computer outside of the body. After proving that the device can be implanted submucosally and transmit data, further experiments can now be performed, primarily with an electrogastrography (EGG) instrument and implantable device with tissue stimulation capability.
- Publication type
- Journal Article MeSH
A microfabricated pneumatic electrospray nebulizer has been developed and evaluated using computer simulations and experimental measurements of the MS signals. The microdevice under development is designed for electrospray MS interfacing without the need to fabricate an electrospray needle and can be used as a disposable or an integral part of a reusable system. The design of the chip layout was supported by computational fluid dynamics simulations. The tested microdevices were fabricated in glass using conventional photolithography, followed by wet chemical etching and thermal bonding. The performance of the microfabricated nebulizer was evaluated by means of TOF-MS with a peptide mixture. It was demonstrated that the nebulizer, operating at supersonic speed of the nebulizing gas, produced very stable nanospray (900 nL/min) as documented by less than 0.1% (SE) fluctuation in total mass spectrometric signal intensity.
A microfluidic cell capture device was designed, fabricated, evaluated by numerical simulations and validated experimentally. The cell capture device was designed with a minimal footprint compartment comprising internal micropillars with the goal to obtain a compact, integrated bioanalytical system. The design of the device was accomplished by computational fluid dynamics (CFD) simulations. Various microdevice designs were rapidly prototyped in poly-dimethylsiloxane using conventional soft lithograpy technique applying micropatterned SU-8 epoxy based negative photoresist as moulding replica. The numerically modeled flow characteristics of the cell capture device were experimentally validated by tracing and microscopic recording the flow trajectories using yeast cells. Finally, we give some perspectives on how CFD modeling can be used in the early stage of microfluidics-based cell capture device development.
Microreactor technology is an interdisciplinary field that combines science and engineering. This new concept in production, analysis and research is finding increasing application in many different fields. Benefits of this new technology pose a vital influence on chemical industry, biotechnology, the pharmaceutical industry and medicine, life science, clinical and environmental diagnostic. In the last few years, together with microplant development, a great part of research investigation is focused on integrated micro-systems, the so called micro-total-analysis-systems (μ-TAS) or lab-on-chip (LOC). They are devices that perform sampling, sample preparation, detection and date processing in integrated model. Cell sorting, cell lysis, single cell analysis and non-destructive single cell experiments on just one microreactor, makes the LOC platform possible. Clinical diagnostic devices are also leaning towards completely integrated, multiple sophisticated biochemical analyses (PCR amplification, cell lysis, separation and detection) all on a single platform and in real time. Special attention is also paid to the usage of microdevices in tissue. Tissue engineering is one of the most promising fields that can lead to in vitro tissue and organ reconstruction ready for implantation and microdevices can be used to promote the migration, proliferation and the differentiation of cells in controlled situations.
- MeSH
- Biophysics MeSH
- Biomedical Technology MeSH
- Biomedical Research MeSH
- Equipment Design MeSH
- DNA analysis MeSH
- Financing, Organized MeSH
- Immunoassay methods instrumentation MeSH
- Metabolomics methods MeSH
- Patch-Clamp Techniques instrumentation MeSH
- Microchemistry instrumentation MeSH
- Microchip Analytical Procedures MeSH
- Microfluidics methods instrumentation MeSH
- Microfluidic Analytical Techniques methods MeSH
- Microtechnology methods instrumentation MeSH
- Miniaturization methods instrumentation MeSH
- Polymerase Chain Reaction methods instrumentation MeSH
- Cell Separation methods instrumentation MeSH
- Tissue Engineering methods instrumentation MeSH
- Publication type
- Review MeSH
elektronický časopis
- Conspectus
- Biotechnologie. Genetické inženýrství
- NML Fields
- biomedicínské inženýrství
- NML Publication type
- elektronické časopisy
Do DNA laboratoří začíná vstupovat „biočipová“ technologie, a to již nejen na úrovni prototypů, ale komerčně vyráběných „přístrojů“, které naznačují ohromný výkonnostní skok, ke kterému může v dohledné budoucnosti dojít. Miniaturizace proniká do většiny procesů charakterizujících DNA laboratoře. Jsou to především mikročipy využí- vající hybridizaci nukleových kyselin, ale byly již vyvinuty mikročipy, v nichž může probíhat polymerázová řetězová reakce nebo separace buněk. Funkce těchto mikropřístrojů je souběžně s jejich vývojem ověřována na reálných úkolech, které jsou s jejich pomocí řešeny.
„Biochips“ technology starts entering DNA laboratories and not only as prototypes, that show fascinating increase in effectivity which can change our nearest future. Practically all processes used in DNA laboratories are to be involved. Hybridization reaction is the most often used principle in microchips, but already polymerase chain reaction (PCR) has been miniaturized to the microchip level and also separation of blood cells. Function of these newly developed microdevices in being verified by solving practical problems in diagnostics.
