• Klíčová slova
• PYCNOGENOL SALVEA,
• MeSH
• biflavonoidy MeSH
• rostlinné extrakty MeSH

• Klíčová slova
• PYCNOGENOL,
• MeSH
• antioxidancia MeSH
• biflavonoidy MeSH

• Klíčová slova
• PYCNOGENOL,
• MeSH
• antioxidancia MeSH
• potravní doplňky MeSH

• Klíčová slova
• PYCNOGENOL (SLOVAKOFARMA),
• MeSH
• farmakoterapie metody   MeSH
• léčivé rostliny MeSH
• oxidační stres MeSH

• MeSH
• biflavonoidy MeSH
• lidé MeSH
• potravní doplňky MeSH
• Check Tag
• lidé MeSH

• Klíčová slova
• pycnogel, oraclin,
• MeSH
• antioxidancia MeSH
• borovice MeSH
• nemoci parodontu prevence a kontrola   MeSH
• potravní doplňky MeSH
• rostlinné extrakty MeSH

Our purpose in this randomized, double blind, placebo controlled study was to find out the possible effect of a polyphenolic pine bark extract, Pycnogenol® (Pyc) on the level of 8-oxo-7,8-dihydroguanine (8-oxoG) as representative of oxidative damage to DNA and on the DNA repair ability of elderly people. According to our results, three months of Pyc administration had no effect on the level of oxidative damage to DNA or on repair ability, but we found a relationship between the level of 8-oxoG and repair ability of DNA in this group. To conclude, even if the positive effect of Pyc was not confirmed in the case of elderly people it is important to highlight the necessity of further investigations about the mechanisms of Pyc acting on different age groups.

• MeSH
• artróza kolenních kloubů metabolismus   MeSH
• borovice MeSH
• dvojitá slepá metoda MeSH
• financování organizované MeSH
• flavonoidy farmakologie   MeSH
• guanin analogy a deriváty   metabolismus   MeSH
• kometový test MeSH
• lidé středního věku MeSH
• lidé MeSH
• oprava DNA účinky léků   MeSH
• oxidační stres účinky léků   MeSH
• poškození DNA účinky léků   MeSH
• randomizované kontrolované studie jako téma MeSH
• rostlinné extrakty farmakologie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH

The work is focused on clarifying the impact of diabetes and natural plant polyphenols contained in Pycnogenol® (PYC) on the activity and synthesis of Cu/Zn-SOD and synthesis of nNOS and eNOS in the cerebellum and cerebral cortex in rats with induced diabetes. Rats included in the study (n=38) were divided into three groups: the controls (C), (n=7), untreated diabetics (D) (n=19) and diabetic rats treated with PYC (DP) (n=12). Diabetes significantly decreased synthesis, as well as the activity of Cu/Zn-SOD in both studied parts of the brain. PYC significantly increased the synthesis of Cu/Zn-SOD but had no effect on its activity. Diabetes also reduced the synthesis of nNOS in cerebral cortex and administered PYC elevated its amount to the level of controls. In the cerebellum, diabetes does not affect the synthesis of nNOS and PYC reduces synthesis of nNOS. Diabetes as well as PYC had no influence on the synthesis of eNOS in both, the cerebellum and cerebral cortex. PYC modulated level of Cu/Zn-SOD and nNOS in cerebellum and cerebral cortex of diabetic rats, but in a different way.

• MeSH
• borovice MeSH
• experimentální diabetes mellitus enzymologie   MeSH
• fenoly farmakologie   MeSH
• flavonoidy farmakologie   MeSH
• krysa rodu rattus MeSH
• mozeček enzymologie   MeSH
• mozková kůra enzymologie   MeSH
• rostlinné extrakty farmakologie   MeSH
• superoxiddismutasa metabolismus   MeSH
• synthasa oxidu dusnatého metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH

Asian sand dust (ASD), also called China dust or yellow dust, mainly occurs in East Asia during spring and autumn. Because ASD enters the body mainly through the respiratory system, it can cause respiratory disorders or worsen underlying diseases. Because of this, it has become an important health concern that threatens the well-being of humans and animals. In this study, we investigated the effects of 15 and 30 mg/kg of Pycnogenol (PYC15 and 30 groups), a pine bark extract, on ASD-induced pulmonary inflammation in mice. We evaluated the inflammatory cell counts, inflammatory cytokines, and matrix-metalloproteinase (MMP)-9 expression in animal models. PYC administration significantly decreased inflammatory cell infiltration into lung tissue; this was accompanied by a reduction in the levels of proinflammatory mediators including interleukin (IL)-1β (P < 0.01), IL-6 (P < 0.01) and tumour necrosis factor-α (P < 0.01) in bronchoalveolar lavage fluids of ASD-exposed mice (ASD group). Histological analysis revealed that PYC suppressed ASD-induced pulmonary inflammation. Moreover, PYC suppressed the levels of matrix-metalloproteinase (MMP)-9 in the lung tissue of ASD-exposed mice, indicating that PYC reduced ASD-induced pulmonary inflammation by suppressing MMP-9. Together, these results indicate that PYC as the potential to treat ASD-driven pulmonary inflammation.