• MeSH
• Coloring Agents metabolism   MeSH
• Basidiomycota metabolism   MeSH
• Biodegradation, Environmental MeSH
• Textile Industry MeSH

The 16S rRNA sequence analysis and biochemical characteristics were confirmed that the isolated bacterium is Pseudomonas sp. LBC1. The commonly used textile dye, Direct Brown MR has been used to study the fate of biodegradation. Pseudomonas sp. LBC1 showed 90% decolorization of Direct Brown MR (100 mg/L) and textile industry effluent with significant reduction in COD and BOD. The optimum condition for decolorization was 7.0 pH and 40°C. Significant increase in a activity of extracellular laccase suggested their possible involvement in decolorization of Direct Brown MR. Biodegradation metabolites viz. 3,6-dihydroxy benzoic acid, 2-hydroxy-7-aminonaphthol-3-sulfonic acid, and p-dihydroperoxybenzene were identified on the basis of mass spectra and using the 1.10 beta Shimadzu NIST GC-MS library. The Direct Brown MR and textile industry effluent were toxic to Sorghum bicolor and Vigna radiata plants as compared to metabolites obtained after decolorization. The Pseudomonas sp. LBC1 could be useful strain for decolorization and detoxification of textile dyes as well as textile industry effluent.

• MeSH
• Coloring Agents metabolism   toxicity   MeSH
• Biodegradation, Environmental MeSH
• Hydrogen-Ion Concentration MeSH
• Biological Oxygen Demand Analysis MeSH
• Molecular Sequence Data MeSH
• Sewage chemistry   microbiology   MeSH
• Industrial Waste adverse effects   analysis   MeSH
• Pseudomonas genetics   isolation & purification   metabolism   MeSH
• Soil Microbiology MeSH
• Environmental Restoration and Remediation methods   MeSH
• Sorghum drug effects   MeSH
• Textile Industry MeSH
• Publication type
• Journal Article MeSH
• Evaluation Study MeSH
• Research Support, Non-U.S. Gov't MeSH

Azo dyes are used as coloring agent in textile industries at larger scale. As a result, large quantity of dye-enriched waste water is generated which subsequently poses environmental problems. Biological tool involving bacteria having azoreductase enzyme has proved to be more effective and efficient in dye effluent treatment. Current work focuses on Staphylococcus caprae (S. caprae) for degradation and decolorization of Reactive Red-195 (RR-195) azo dye. For this purpose, factors such as pH, temperature, inoculums, carbon and nitrogen sources, and dye concentrations have been optimized for maximum decolorization and degradation. S. caprae (4 mg/mL) efficiently resulted into 90% decolorization of RR-195 dye under static condition at 100 μg/mL concentration, 30 °C and pH 7.0 at a 12-h contact period. FTIR analysis has revealed the formation of new functional groups in the treated dye such as O-H stretch at 3370 cm-1, C-H band stretching at 2928 cm-1, and new band at 1608 cm-1 which specify the degradation of aromatic ring, 1382 and 1118 cm-1 represents desulfonated peaks. Biodegraded metabolites of RR-195 dye such as phenol, 3, 5-di-tert-butylphenol, and phthalic acid have been identified respectively that find industrial applications. Phytotoxicity test has shown non-toxic effects of treated dye on germination of Vigna radiata and Triticum aestivum seeds. Further, antibiotic diffusion assay has confirmed the biosafety of S. caprae.

• MeSH
• Azo Compounds * metabolism   toxicity   MeSH
• Coloring Agents * metabolism   MeSH
• Biodegradation, Environmental * MeSH
• Water Pollutants, Chemical metabolism   MeSH
• Nitrogen metabolism   MeSH
• Hydrogen-Ion Concentration MeSH
• Wastewater * microbiology   chemistry   MeSH
• Industrial Waste MeSH
• Staphylococcus capitis metabolism   isolation & purification   MeSH
• Staphylococcus metabolism   MeSH
• Temperature MeSH
• Textiles MeSH
• Textile Industry MeSH
• Carbon metabolism   MeSH
• Publication type
• Journal Article MeSH

Laccases are enzymes with a broad range of biotechnological applications and have, for example, the ability to oxidize many xenobiotics including synthetic dyes. In order to obtain an efficient laccase for the decolorization of dyes which spoil wastewater from the textile industry, genes encoding three various laccase enzymes were expressed in Saccharomyces cerevisiae. The expression of laccases from ascomycete Myceliophthora thermophila (MtL), and two basidiomycetes Trametes versicolor (TvL) and Trametes trogii (TtL) was optimized via selection of plasmids, promoters, media composition, and cultivation conditions. For the first time, the activity of the three secreted laccases was directly compared with the use of various substrates, including different dyes and a wastewater sample. A strong constitutive ADH1 promoter, minimal growth medium, optimized combination of copper and organic nitrogen source, and low cultivation temperature were shown to significantly increase the yields and relative activities of secreted laccases. Heterologous expression of three fungal laccases was successfully achieved in S. cerevisiae being the highest for MtL and the lowest for TvL. MtL, and particularly TtL, showed the decolorization capacity. This is the first report which compared decolorization of synthetic dyes and wastewater by several recombinant laccases and suggested MtL and TtL to be applicable in the ecofriendly enzymatic treatment of colored industry effluent. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:69-80, 2018.

• MeSH
• Coloring Agents chemistry   MeSH
• Nitrogen chemistry   MeSH
• Culture Media chemistry   MeSH
• Laccase chemistry   genetics   MeSH
• Wastewater chemistry   MeSH
• Oxidation-Reduction MeSH
• Saccharomyces cerevisiae enzymology   genetics   MeSH
• Textile Industry MeSH
• Trametes chemistry   enzymology   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

• Keywords
• zpomalovače hoření,
• MeSH
• Organophosphates adverse effects   toxicity   MeSH
• Flame Retardants adverse effects   toxicity   MeSH
• Environmental Exposure MeSH

• Conspectus
• Lékařské vědy. Lékařství
• NML Fields
• environmentální vědy
• toxikologie
• environmentální vědy
• NML Publication type
• publikace WHO

• Keywords
• zpomalovače hoření,
• MeSH
• No-Observed-Adverse-Effect Level MeSH
• Organophosphates toxicity   MeSH
• Organophosphorus Compounds toxicity   MeSH
• Flame Retardants toxicity   MeSH
• Environmental Exposure MeSH

• Conspectus
• Lékařské vědy. Lékařství
• NML Fields
• toxikologie
• hygiena
• pracovní lékařství
• environmentální vědy
• NML Publication type
• publikace WHO