antifouling activity Dotaz Zobrazit nápovědu
The contact of blood with artificial materials generally leads to immediate protein adsorption (fouling), which mediates subsequent biological processes such as platelet adhesion and activation leading to thrombosis. Recent progress in the preparation of surfaces able to prevent protein fouling offers a potential avenue to mitigate this undesirable effect. In the present contribution, we have prepared several types of state-of-the-art antifouling polymer brushes on polycarbonate plastic substrate, and investigated their ability to prevent platelet adhesion and thrombus formation under dynamic flow conditions using human blood. Moreover, we compared the ability of such brushes--grafted on quartz via an adlayer analogous to that used on polycarbonate--to prevent protein adsorption from human blood plasma, assessed for the first time by means of an ultrahigh frequency acoustic wave sensor. Results show that the prevention of such a phenomenon constitutes one promising route toward enhanced resistance to thrombus formation, and suggest that antifouling polymer brushes could be of service in biomedical applications requiring extensive blood-material surface contact.
- MeSH
- adhezivita trombocytů účinky léků MeSH
- křemen chemie MeSH
- lidé MeSH
- polykarboxylátový cement chemie farmakologie MeSH
- povrchové vlastnosti * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Implantable sensor devices require coatings that efficiently interface with the tissue environment to mediate biochemical analysis. In this regard, bioinspired polymer hydrogels offer an attractive and abundant source of coating materials. However, upon implantation these materials generally elicit inflammation and the foreign body reaction as a consequence of protein fouling on their surface and concomitant poor hemocompatibility. In this report we investigate a strategy to endow chitosan hydrogel coatings with antifouling properties by the grafting of polymer brushes in a "grafting-from" approach. Chitosan coatings were functionalized with polymer brushes of oligo(ethylene glycol) methyl ether methacrylate and 2-hydroxyethyl methacrylate using photoinduced single electron transfer living radical polymerization and the surfaces were thoroughly characterized by XPS, AFM, water contact angle goniometry, and in situ ellipsometry. The antifouling properties of these new bioinspired hydrogel-brush coatings were investigated by surface plasmon resonance. The influence of the modifications to the chitosan on hemocompatibility was assessed by contacting the surfaces with platelets and leukocytes. The coatings were hydrophilic and reached a thickness of up to 180 nm within 30 min of polymerization. The functionalization of the surface with polymer brushes significantly reduced the protein fouling and eliminated platelet activation and leukocyte adhesion. This methodology offers a facile route to functionalizing implantable sensor systems with antifouling coatings that improve hemocompatibility and pave the way for enhanced device integration in tissue.
- MeSH
- aktivace trombocytů účinky léků MeSH
- biokompatibilní potahované materiály chemie farmakologie MeSH
- biosenzitivní techniky metody MeSH
- buněčná adheze účinky léků MeSH
- chitosan chemie MeSH
- hydrogely chemie farmakologie MeSH
- implantabilní infuzní pumpy MeSH
- leukocyty cytologie účinky léků MeSH
- lidé MeSH
- methakryláty chemie MeSH
- polyethylenglykoly chemie MeSH
- polymerizace MeSH
- primární buněčná kultura MeSH
- trombocyty účinky léků MeSH
- volné radikály MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Currently, one of the most promising treatments of lipopolysaccharides (LPS)-induced sepsis is based on hemofiltration. Nevertheless, proteins rapidly adsorbed on the artificial surface of membranes which leads to activation of coagulation impairing effective scavenging of the endotoxins. To overcome this challenge, we designed polymer-brush-coated microparticles displaying antifouling properties and functionalized them with polymyxin B (PMB) to specifically scavenge LPS the most common endotoxin. Poly[( N-(2-hydroxypropyl) methacrylamide)- co-(carboxybetaine methacrylamide)] brushes were grafted from poly(glycidyl methacrylate) microparticles using photoinduced single-electron transfer living radical polymerization (SET-LRP). Notably, only parts-per-million of copper catalyst were necessary to achieve brushes able to repel adsorption of proteins from blood plasma. The open porosity of the particles, accessible to polymerization, enabled us to immobilize sufficient PMB to selectively scavenge LPS from blood plasma.
- MeSH
- adsorpce MeSH
- akrylamidy metabolismus MeSH
- biokompatibilní potahované materiály farmakologie MeSH
- bioznečištění prevence a kontrola MeSH
- epoxidové sloučeniny metabolismus MeSH
- krevní plazma metabolismus MeSH
- lidé MeSH
- lipopolysacharidy metabolismus MeSH
- methakryláty metabolismus MeSH
- polymerizace účinky léků MeSH
- polymery chemie MeSH
- polymyxin B farmakologie MeSH
- povrchové vlastnosti účinky léků MeSH
- proteiny metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The antifouling, antimicrobial, elution behavior, skin irritant, and cytotoxicity properties of water-soluble phosphate glass on stainless steel were evaluated. Water-soluble phosphate glass samples with 35% Cu (mol/mol) were prepared by altering the network modifier (Na2O, K2O) and network former (P2O5, B2O3) compositions. The materials were melted at temperatures within the range of 850-950 °C. The melt was then quenched and ground into fine particles using a twin roll mill. The resulting water-soluble glasses were prepared as glass frit (size < 100 μm) using a sieve. The amorphous phase was determined by X-ray diffraction and differential thermal analysis. Water-soluble glasses with a reduced Cu ion elution rate of 1.2 ppm per week were formed because the chemical resistances of the formulated glasses improved as the P2O5 content decreased and the B2O3 content increased. To test its antifouling properties, the glass frit was mixed with paint and coated onto a STS316L sheet. The surface roughness was increased markedly from 1.4 to 19.2 nm, increasing the specific surface area for antimicrobial activity. It was demonstrated that the proposed method was able to form noncytotoxic, nonirritant, water-soluble glasses with 99.9% antimicrobial activity against Staphylococcus aureus. These results suggest that water-soluble phosphate glass on STS316L sheets could be useful in filtration plants.
- MeSH
- antibakteriální látky chemie farmakologie MeSH
- bioznečištění prevence a kontrola MeSH
- čištění vody přístrojové vybavení MeSH
- difrakce rentgenového záření MeSH
- filtrace přístrojové vybavení MeSH
- fosfáty chemie farmakologie MeSH
- králíci MeSH
- kůže účinky léků MeSH
- sklo chemie MeSH
- Staphylococcus aureus účinky léků růst a vývoj MeSH
- teplota MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Non-specific protein adsorption (fouling) triggers a number of deleterious events in the application of biomaterials. Antifouling polymer brushes successfully suppress fouling, however for some coatings an extremely high variability of fouling for different donors remains unexplained. The authors report that in the case of poly(2-hydroxyethyl methacrylate) (poly(HEMA)) this variability is due to the complement system activation that causes massive acceleration in the fouling kinetics of blood plasma. Using plasma from various donors, the fouling kinetics on poly(HEMA) is analyzed and correlated with proteins identified in the deposits on the surface and with the biochemical compositions of the plasma. The presence of complement components in fouling deposits and concentrations of C3a in different plasmas indicate that the alternative complement pathway plays a significant role in the fouling on poly(HEMA) through the "tick-over" mechanism of spontaneous C3 activation. The generated C3b binds to the poly(HEMA) surface and amplifies complement activation locally. Heat-inactivated plasma prevents accelerated fouling kinetics, confirming the central role of complement activation. The results highlight the need to take into account the variability between individuals when assessing interactions between biomaterials and blood plasma, as well as the importance of the mechanistic insight that can be gained from protein identification.
Cells are continuously sensing their microenvironment and subsequently respond to different physicochemical cues by the activation or inhibition of different signaling pathways. To study a very complex cellular response, it is necessary to diminish background environmental influences and highlight the particular event. However, surface-driven nonspecific interactions of the abundant biomolecules from the environment influence the targeted cell response significantly. Yes-associated protein (YAP) translocation may serve as a marker of human hepatocellular carcinoma (Huh7) cell responses to the extracellular matrix and surface-mediated stresses. Here, we propose a platform of tunable functionable antifouling poly(carboxybetain) (pCB)-based brushes to achieve a molecularly clean background for studying arginine, glycine, and aspartic acid (RGD)-induced YAP-connected mechanotransduction. Using two different sets of RGD-functionalized zwitterionic antifouling coatings with varying compositions of the antifouling layer, a clear correlation of YAP distribution with RGD functionalization concentrations was observed. On the other hand, commonly used surface passivation by the oligo(ethylene glycol)-based self-assembled monolayer (SAM) shows no potential to induce dependency of the YAP distribution on RGD concentrations. The results indicate that the antifouling background is a crucial component of surface-based cellular response studies, and pCB-based zwitterionic antifouling brush architectures may serve as a potential next-generation easily functionable surface platform for the monitoring and quantification of cellular processes.
- MeSH
- akrylamidy chemie MeSH
- biokompatibilní potahované materiály chemie MeSH
- bioznečištění prevence a kontrola MeSH
- buněčný převod mechanických signálů * MeSH
- extracelulární matrix metabolismus MeSH
- lidé MeSH
- mechanický stres MeSH
- nádorové buněčné linie MeSH
- oligopeptidy chemie MeSH
- protoonkogenní proteiny c-yes metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Whenever an artificial surface comes into contact with blood, proteins are rapidly adsorbed onto its surface. This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation of complement or the coagulation cascade and adhesion of leukocytes and platelets leading to thrombus formation. Thus, considerable efforts are directed towards the preparation of fouling-resistant surfaces with the best possible hemocompatibility. Herein, a comprehensive hemocompatibility study after heparinized blood contact with seven polymer brushes prepared by surface-initiated atom transfer radical polymerization is reported. The resistance to fouling is quantified and thrombus formation and deposition of blood cellular components on the coatings are analyzed. Moreover, identification of the remaining adsorbed proteins is performed via mass spectroscopy to elucidate their influence on the surface hemocompatibility. Compared with an unmodified glass surface, the grafting of polymer brushes minimizes the adhesion of platelets and leukocytes and prevents the thrombus formation. The fouling from undiluted blood plasma is reduced by up to 99%. Most of the identified proteins are connected with the initial events of foreign body reaction towards biomaterial (coagulation cascade proteins, complement component, and inflammatory proteins). In addition, several proteins that are not previously linked with blood-biomaterial interaction are presented and discussed.
Tributyltin (TBT), as antifouling paints, is widely present in aquatic environment, but little is known regarding the toxicity of TBT on fish brain. In this study, the effects of exposure to TBT on the antioxidant defense system, Na(+) -K(+) -ATPase activity, neurological enzymes activity and Hsp 70 protein level in brain of juvenile common carp (Cyprinus carpio) were studied. Fish were exposed to sublethal concentrations of TBT (5, 10 and 20 μg/L) for 7 days. Based on the results, with increasing concentrations of TBT, oxidative stress was apparent as reflected by the significant higher levels of oxidative indices, as well as the significant inhibition of all antioxidant enzymes activities. Besides, the activities of Acetylcholinesterase (AChE), Monoamine oxidases (MAO) and Na(+) -K(+) -ATPase were significantly inhibited after exposure to TBT with higher concentrations. In addition, the levels of Hsp 70 protein were evaluated under TBT stress with dose-depended manner. These results suggest that selected physiological responses in fish brain could be used as potential biomarkers for monitoring residual organotin compounds present in aquatic environment.
- MeSH
- acetylcholinesterasa metabolismus MeSH
- antioxidancia metabolismus MeSH
- biologické markery metabolismus MeSH
- chemické látky znečišťující vodu toxicita MeSH
- kapři * genetika metabolismus MeSH
- mozek účinky léků metabolismus MeSH
- oxidační stres účinky léků MeSH
- sodíko-draslíková ATPasa metabolismus MeSH
- trialkylcínové sloučeniny toxicita MeSH
- věkové faktory MeSH
- vodní hospodářství MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
In this study, the chronic toxic effects of tributyltin (TBT), an antifouling paints commonly present in surface and ground water, on morphological indices, reactive oxygen species (ROS) generation, and ATPase activity and heat shock protein (Hsp) 70 protein in tissues (liver, gill, and white muscle) of common carp were investigated. Fish were exposed at sublethal concentrations of TBT (75 ng/L, 0.75 μg/L, and 7.5 μg/L) for 60 days. When compared with the control, there was significant lower condition factor in fish exposed at the higher concentration of TBT. ROS levels in three tissues increased significantly at higher TBT concentrations (0.75 and 7.5 μg/L). The hepatic antioxidant enzymes (total antioxidative capacity and superoxide dismutase) activities were induced at higher concentrations (0.75 μg/L) of TBT. When compared with the hepatic antioxidant enzymes activities in fish exposed to 0.75 μg/L of TBT, there was a decreasing trend in those exposed to TBT with a concentration of 7.5 μg/L. However, all the antioxidant enzymes activities were significantly inhibited in gill and muscle of fish exposed to higher TBT concentrations (0.75 and 7.5 μg/L). Moreover, there was significant lower Na-K-ATPase in three tissues after long-term exposure to higher concentration of TBT, but a significant higher Hsp70 protein levels was observed. In short, environmental concentrations of TBT could not induce obvious impacts on fish, but long-term exposure to higher concentrations of TBT could affect seriously the health status of fish. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 937-944, 2016.
- MeSH
- antioxidancia metabolismus MeSH
- biologické markery metabolismus MeSH
- chemické látky znečišťující vodu toxicita MeSH
- játra účinky léků enzymologie MeSH
- kapři metabolismus MeSH
- orgánová specificita MeSH
- proteiny tepelného šoku HSP70 metabolismus MeSH
- rybí proteiny metabolismus MeSH
- sodíko-draslíková ATPasa metabolismus MeSH
- superoxiddismutasa metabolismus MeSH
- trialkylcínové sloučeniny toxicita MeSH
- žábry metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Nonthrombogenic modifications of membranes for extracorporeal membrane oxygenators (ECMOs) are of key interest. The absence of hemocompatibility of these membranes and the need of anticoagulation of patients result in severe and potentially life-threatening complications during ECMO treatment. To address the lack of hemocompatibility of the membrane, surface modifications are developed, which act as barriers to protein adsorption on the membrane and, in this way, prevent activation of the coagulation cascade. The modifications are based on nonionic and zwitterionic polymer brushes grafted directly from poly(4-methyl-1-pentene) (TPX) membranes via single electron transfer-living radical polymerization. Notably, this work introduces the first example of well-controlled surface-initiated radical polymerization of zwitterionic brushes. The antifouling layers markedly increase the recalcification time (a proxy of initiation of coagulation) compared to bare TPX membranes. Furthermore, platelet and leukocyte adhesion is drastically decreased, rendering the ECMO membranes hemocompatible.
