Transmission of phytoplasmas from naturally infected plant host species using the parasitic plant Cuscuta spp. (dodder) to Catharanthus roseus (Madagascar periwinkle) is an effective way to maintain a wide range of phytoplasmas for further research. Here, we describe transmission via dodder from an infected medicinal plant Rehmannia glutinosa var. purpurea and from a symptomatic redcurrant plant (Ribes spp.) to C. roseus indicator plants using a "stable bridges" method. In both cases, typical symptoms of phytoplasma disease on periwinkle plants were obtained: virescent flowers with an increased number of axillary shoots and smaller leaves after transmission from R. glutinosa, and greening petals (virescence) after transmission from Ribes spp. Phytoplasmas could be detected in donor and recipient plants by electron microscopy and by polymerase chain reaction (PCR) assays using universal phytoplasma primer pairs. Restriction fragment length polymorphism analyses of PCR fragments can also be used to confirm the identity of the phytoplasmas from donor and recipient plants.

• MeSH
• Catharanthus růst a vývoj   mikrobiologie   MeSH
• Cuscuta růst a vývoj   mikrobiologie   MeSH
• nemoci rostlin mikrobiologie   MeSH
• Phytoplasma * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• MeSH
• Cuscuta * MeSH
• fytoterapie MeSH
• léčivé rostliny MeSH

Cuscuta europaea and Cuscuta campestris (Cuscutaceae) are annual parasitic plants which wrap around host plants and penetrate their tissues using haustoria. There are more than 150 species of Cuscuta worldwide; C. europaea and C. campestris are the most common species in the Czech Republic and Slovakia. In this study phenolics were identified in methanolic extracts of these species by HPLC and MS. Among others, caffeic acid, p-coumaric acid, isorhamnetin and isorhamnetin 3-O- -glucoside were found in the extracts.

• MeSH
• Cuscuta chemie   MeSH
• fenoly analýza   MeSH
• rostlinné extrakty chemie   MeSH
• vysokoúčinná kapalinová chromatografie metody   využití   MeSH

• MeSH
• arginin farmakologie   imunologie   účinky léků   MeSH
• Cuscuta imunologie   účinky léků   MeSH
• erektilní dysfunkce terapie   MeSH
• Ginkgo biloba imunologie   účinky léků   MeSH
• inzerce jako téma etika   metody   zákonodárství a právo   MeSH
• kontrola potravin metody   zákonodárství a právo   MeSH
• lidé MeSH
• potravní doplňky škodlivé účinky   využití   MeSH
• sexuologie metody   MeSH
• ženšen imunologie   účinky léků   MeSH
• Check Tag
• lidé MeSH