• MeSH
• infekce vyvolané poxviry MeSH
• Poxviridae izolace a purifikace   patogenita   MeSH
• Geografické názvy
• střední Afrika MeSH

• MeSH
• infekce vyvolané poxviry MeSH
• Poxviridae izolace a purifikace   patogenita   MeSH
• Geografické názvy
• střední Afrika MeSH

• MeSH
• infekce vyvolané poxviry patofyziologie   MeSH
• Poxviridae izolace a purifikace   MeSH
• velbloudi virologie   MeSH

• MeSH
• infekce vyvolané poxviry patofyziologie   MeSH
• Poxviridae izolace a purifikace   MeSH
• velbloudi virologie   MeSH

• MeSH
• chloroform aplikace a dávkování   farmakologie   MeSH
• infekce vyvolané poxviry MeSH
• kozy MeSH
• ovce MeSH
• Poxviridae fyziologie   MeSH

• MeSH
• infekce vyvolané poxviry etiologie   MeSH

Outbreaks of koi sleepy disease (KSD) caused by carp edema virus (CEV) may seriously affect populations of farmed common carp, one of the most important fish species for global food production. The present study shows further evidence for the involvement of CEV in outbreaks of KSD among carp and koi populations: in a series of infection experiments, CEV from two different genogroups could be transmitted to several strains of naïve common carp via cohabitation with fish infected with CEV. In recipient fish, clinical signs of KSD were induced. The virus load and viral gene expression results confirm gills as the target organ for CEV replication. Gill explants also allowed for a limited virus replication in vitro. The in vivo infection experiments revealed differences in the virulence of the two CEV genogroups which were associated with infections in koi or in common carp, with higher virulence towards the same fish variety as the donor fish. When the susceptibility of different carp strains to a CEV infection and the development of KSD were experimentally investigated, Amur wild carp showed to be relatively more resistant to the infection and did not develop clinical signs for KSD. However, the resistance could not be related to a higher magnitude of type I IFN responses of affected tissues. Despite not having a mechanistic explanation for the resistance of Amur wild carp to KSD, we recommend using this carp strain in breeding programs to limit potential losses caused by CEV in aquaculture.

• MeSH
• infekce vyvolané poxviry veterinární   virologie   MeSH
• kapři virologie   MeSH
• kůže virologie   MeSH
• nemoci ryb virologie   MeSH
• Poxviridae * MeSH
• vodní hospodářství metody   MeSH
• žábry virologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• infekce vyvolané poxviry MeSH
• pravé neštovice MeSH
• Publikační typ
• letáky MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• infekční lékařství

• O autorovi
• Smrt pravých neštovic (2010 : Praha, Česko) Autorita

• MeSH
• exantém MeSH
• infekce vyvolané poxviry MeSH

Koi sleepy disease (KSD) is a disease with increasing importance in global common carp aquaculture. Despite the fact that carp edema virus (CEV) is most likely the causative agent of KSD, the disease often presents itself as multifactorial with several parasites and bacteria species present on gills, skin or in internal organs. Therefore, in this study, we analysed and presented initial results on an interaction of flavobacteria and CEV in the development of clinical KSD in carp suffering from proliferative gill disease. We examined selected field samples from Germany and Hungary and confirmed the presence of CEV and flavobacteria co-infections in subset of the samples. In several infection experiments, we studied the transfer and dynamics of both infections. Furthermore, we analysed which Flavobacterium species could be isolated from KSD-affected fish and concluded that Flavobacterium branchiophilum is a possible copathogen. Antibiotic treatment experiments showed that CEV seems to be the primary pathogen causing an insult to the gills of carp and by these enabling other pathogens, including F. branchiophilum, to establish co-infections. Despite the fact that F. branchiophilum co-infection is not required for the development of clinical KSD, it could contribute to the pathological changes recorded during the outbreaks.

• MeSH
• Flavobacterium fyziologie   MeSH
• infekce bakteriemi čeledi Flavobacteriaceae farmakoterapie   mikrobiologie   veterinární   MeSH
• infekce vyvolané poxviry farmakoterapie   mikrobiologie   veterinární   MeSH
• kapři * MeSH
• koinfekce farmakoterapie   mikrobiologie   veterinární   virologie   MeSH
• nemoci ryb farmakoterapie   mikrobiologie   virologie   MeSH
• Poxviridae účinky léků   fyziologie   MeSH
• žábry mikrobiologie   patologie   virologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Maďarsko MeSH
• Německo MeSH