A taxonomic study of 24 Gram-stain-negative rod-shaped bacteria originating from the Antarctic environment is described. Phylogenetic analysis using 16S rRNA gene sequencing differentiated isolated strains into two groups belonging to the genus Flavobacterium. Group I (n=20) was closest to Flavobacterium aquidurense WB 1.1-56T (98.3% 16S rRNA gene sequence similarity) while group II (n=4) showed Flavobacterium hydatis DSM 2063T as its nearest neighbour (98.5-98.9% 16S rRNA gene sequence similarity). Despite high 16S rRNA gene sequence similarity, these two groups represented two distinct novel species as shown by phenotypic traits and low genomic relatedness assessed by rep-PCR fingerprinting, DNA-DNA hybridization and whole-genome sequencing. Common to representative strains of both groups were the presence of major menaquinone MK-6 and sym-homospermidine as the major polyamine. Common major fatty acids were C15:0 iso, C15:1 iso G, C15:0 iso 3-OH, C17:0 iso 3OH and Summed Feature 3 (C16:1ω7c/C16:1ω6c). Strain CCM 8828T (group I) contained phosphatidylethanolamine, three unidentified lipids lacking a functional group, three unidentified aminolipids and single unidentified glycolipid in the polar lipid profile. Strain CCM 8825T (group II) contained phosphatidylethanolamine, eight unidentified lipids lacking a functional group, three unidentified aminolipids and two unidentified glycolipids in the polar lipid profile. These characteristics corresponded to characteristics of the genus Flavobacterium. The obtained results showed that the analysed strains represent novel species of the genus Flavobacterium, for which the names Flavobacterium circumlabens sp. nov. (type strain CCM 8828T=P5626T=LMG 30617T) and Flavobacterium cupreum sp. nov. (type strain CCM 8825T=P2683T=LMG 30614T) are proposed.

• MeSH
• chinony analýza   MeSH
• DNA bakterií genetika   MeSH
• druhová specificita MeSH
• Flavobacterium chemie   klasifikace   genetika   fyziologie   MeSH
• fylogeneze * MeSH
• genom bakteriální genetika   MeSH
• lipidy analýza   MeSH
• mastné kyseliny analýza   MeSH
• polyaminy analýza   MeSH
• RNA ribozomální 16S genetika   MeSH
• sekvenční analýza DNA MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Antarktida MeSH

A group of rod-shaped, aerobic, Gram-stain-negative, gliding bacteria producing flexirubin-type pigment was isolated from environmental samples collected in Antarctica in 2009-2014. Phylogenetic analysis of the almost complete 16S rRNA gene sequences revealed two separated branches belonging to the genus Flavobacterium. Group I (n=8), represented by strain CCM 8826T, shared the highest sequence similarity to Flavobacterium collinsii 983-08T (98.8 %) and Flavobacterium saccharophilum DSM 1811T (98.4 %), and group II (n=4) represented by strain CCM 8827T shared the highest similarity to Flavobacterium aquidurense WB 1.1-56T (99.6 %). High genetic homogeneity of both groups, separation from each other and from phylogenetically close Flavobacterium species was verified by the rep-PCR fingerprinting method. DNA-DNA hybridization confirmed low genomic relatedness between strain CCM 8826T and F. collinsii 983-08T and F. saccharophilum DSM 1811T (18 and 28 %, respectively) and between strain CCM 8827T and F. aquidurense WB 1.1-56T (27 %). Chemotaxonomic analyses of strains CCM 8826T and CCM 8827T revealed the respiratory quinone to be MK-6, the major identified polar lipid was phosphatidylethanolamine and the predominant polyamine was sym-homospermidine. The common major fatty acids were C15 : 0 iso, C17 : 0 iso 3OH, C15 : 1 iso G, Summed Feature 3 (C16 : 1ω7c/C16 : 1ω6c), C15 : 0 iso 3OH and additionally, C15 : 0 anteiso among group II members. All analyses confirmed that strains of group I and II represent two novel species of the genus Flavobacterium, for which the names Flavobacterium chryseum sp. nov. (type strain CCM 8826T=P3160T=LMG 30615T) and Flavobacterium psychroterrae sp. nov. (type strain CCM 8827T=P3922T=LMG 30616T) are proposed.

• MeSH
• DNA bakterií genetika   MeSH
• Flavobacterium klasifikace   genetika   izolace a purifikace   MeSH
• fosfatidylethanolaminy chemie   MeSH
• fylogeneze * MeSH
• hybridizace nukleových kyselin MeSH
• mastné kyseliny chemie   MeSH
• pigmentace MeSH
• půdní mikrobiologie * MeSH
• RNA ribozomální 16S genetika   MeSH
• sekvenční analýza DNA MeSH
• spermidin analogy a deriváty   chemie   MeSH
• techniky typizace bakterií MeSH
• vitamin K 2 analogy a deriváty   chemie   MeSH
• zastoupení bazí MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Antarktida MeSH

Koi sleepy disease (KSD) is a disease with increasing importance in global common carp aquaculture. Despite the fact that carp edema virus (CEV) is most likely the causative agent of KSD, the disease often presents itself as multifactorial with several parasites and bacteria species present on gills, skin or in internal organs. Therefore, in this study, we analysed and presented initial results on an interaction of flavobacteria and CEV in the development of clinical KSD in carp suffering from proliferative gill disease. We examined selected field samples from Germany and Hungary and confirmed the presence of CEV and flavobacteria co-infections in subset of the samples. In several infection experiments, we studied the transfer and dynamics of both infections. Furthermore, we analysed which Flavobacterium species could be isolated from KSD-affected fish and concluded that Flavobacterium branchiophilum is a possible copathogen. Antibiotic treatment experiments showed that CEV seems to be the primary pathogen causing an insult to the gills of carp and by these enabling other pathogens, including F. branchiophilum, to establish co-infections. Despite the fact that F. branchiophilum co-infection is not required for the development of clinical KSD, it could contribute to the pathological changes recorded during the outbreaks.

• MeSH
• Flavobacterium fyziologie   MeSH
• infekce bakteriemi čeledi Flavobacteriaceae farmakoterapie   mikrobiologie   veterinární   MeSH
• infekce vyvolané poxviry farmakoterapie   mikrobiologie   veterinární   MeSH
• kapři * MeSH
• koinfekce farmakoterapie   mikrobiologie   veterinární   virologie   MeSH
• nemoci ryb farmakoterapie   mikrobiologie   virologie   MeSH
• Poxviridae účinky léků   fyziologie   MeSH
• žábry mikrobiologie   patologie   virologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Maďarsko MeSH
• Německo MeSH

Cold-loving microorganisms developed numerous adaptation mechanisms allowing them to survive in extremely cold habitats, such as adaptation of the cell membrane. The focus of this study was on the membrane fatty acids of Antarctic Flavobacterium spp., and their adaptation response to cold-stress. Fatty acids and cold-response of Antarctic flavobacteria was also compared to mesophilic and thermophilic members of the genus Flavobacterium. The results showed that the psychrophiles produced more types of major fatty acids than meso- and thermophilic members of this genus, namely C15:1 iso G, C15:0 iso, C15:0 anteiso, C15:1ω6c, C15:0 iso 3OH, C17:1ω6c, C16:0 iso 3OH and C17:0 iso 3OH, summed features 3 (C16:1ω7cand/or C16:1ω6c) and 9 (C16:0 10-methyl and/or C17:1 iso ω9c). It was shown that the cell membrane of psychrophiles was composed mainly of branched and unsaturated fatty acids. The results also implied that Antarctic flavobacteria mainly used two mechanisms of membrane fluidity alteration in their cold-adaptive response. The first mechanism was based on unsaturation of fatty acids, and the second mechanism on de novo synthesis of branched fatty acids. The alteration of the cell membrane was shown to be similar for all thermotypes of members of the genus Flavobacterium.

• MeSH
• Flavobacterium fyziologie   MeSH
• fyziologická adaptace * MeSH
• mastné kyseliny metabolismus   MeSH
• nízká teplota * MeSH
• reprodukovatelnost výsledků MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Antarktida MeSH

No significant difference (p > 0.05) was observed in the specific aminopeptidase activity (SAA) developed by Pseudomonas fluorescens, P. putida and Flavobacterium odoratum either growing at pH 5.0-6.5 or at 7 and 12 degrees C. Nevertheless, a significant difference was found when comparing the SAA of these organisms. The SAA of F. odoratum was lower than those of pseudomonads. The 4-nitroaniline test is reliable to estimate the G(-) load of fresh food products.

• MeSH
• aminopeptidasy metabolismus   MeSH
• aniliny metabolismus   MeSH
• bakteriální proteiny metabolismus   MeSH
• bakteriologické techniky MeSH
• Flavobacterium enzymologie   růst a vývoj   MeSH
• koncentrace vodíkových iontů MeSH
• kultivační média MeSH
• Pseudomonas fluorescens enzymologie   růst a vývoj   MeSH
• Pseudomonas putida enzymologie   růst a vývoj   MeSH
• teplota MeSH

• MeSH
• bakteriemie etiologie   farmakoterapie   MeSH
• dospělí MeSH
• Flavobacterium patogenita   MeSH
• katetrizace MeSH
• lidé středního věku MeSH
• lidé MeSH
• vankomycin terapeutické užití   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH

Cíl práce: Identifikace mikroorganismu izolovaného z likvoru u pacientky s nosokomiální purulentní meningitidou. Materiál a metodika: je popsán klinický průběh onemocnění, záchyt gram negativních tyček z likvoru, způsob identifikace a citlivost k antimikrobním látkám. Výsledky: Izolovaný kmen byl identifikován jako Weeksella virosa podle mikroskopické i makroskopické morfologie, růstových a biochemických vlastností, typického antibiogramu a porovnáním s referenčním kmenem. Závěr: Weeksella virosa lze identifikovat jak pomocí firemních diagnostických souprav, tak i konvenčními testy.

Objective: Identification of a microorganism isolated from cerebrospinal fluid in case of nosocomial purulent meningitis. Methods: The clinical course of illness, isolation of gram negative rods from cerebrospinal fluid, the way of identification and susceptibility to antimicrobial agents are desribed. Results: The isolated culture was identified as Weeksella virosahy microscopic and macroscopic morfology, growing abilities and biochemical characteristics, typical antimicrobial susceptibility and by comparing with the national colection strain. Conclusion: The identification of Weeksella virosa is possible by using of commercially produced diagnostics sets, but also by conventional tests.

• MeSH
• cefalosporiny terapeutické užití   MeSH
• Flavobacterium izolace a purifikace   metabolismus   účinky léků   MeSH
• infekce spojené se zdravotní péčí farmakoterapie   mikrobiologie   MeSH
• lidé MeSH
• meningitida bakteriální farmakoterapie   mikrobiologie   MeSH
• mikrobiální testy citlivosti MeSH
• mladiství MeSH
• Check Tag
• lidé MeSH
• mladiství MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

• MeSH
• Flavobacterium izolace a purifikace   MeSH
• infekce spojené se zdravotní péčí prevence a kontrola   MeSH
• lidé MeSH
• novorozenec přenošený MeSH
• novorozenec MeSH
• zvukovod mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• kazuistiky MeSH

• MeSH
• Acinetobacter MeSH
• Alcaligenes MeSH
• bakteriologické techniky metody   normy   MeSH
• Flavobacterium MeSH
• gramnegativní bakteriální infekce diagnóza   MeSH
• Moraxella MeSH
• Pseudomonas MeSH
• Publikační typ
• směrnice MeSH

• Konspekt
• Mikrobiologie
• NLK Obory
• bakteriologie
• chemie, klinická chemie

• MeSH
• farmaceutická chemie MeSH
• Flavobacterium MeSH
• hydrokortison chemická syntéza   MeSH
• metody MeSH
• oxidace-redukce MeSH