In this study we were interested in the vaccine potential of two attenuated mutants of Salmonella enterica serovar Enteritidis for poultry. The first mutant was attenuated by the removal of the whole Salmonella Pathogenicity Island 1 (SPI1) and the second mutant was devoid of the whole SPI2. These 2 mutants were used for oral vaccination of 2 chicken lines; Lohmann Brown and ISA Brown. Chickens were vaccinated orally on day 1 of life, revaccinated on day 21 and challenged on day 42. The challenge was performed either orally or intravenously. Despite a slightly different response between the two chicken lines, both the mutants gave protection to poultry against S. Enteritidis challenge as documented by findings such as the bacterial counts in tissues, spleen weight, antibody production and cytokine response (namely IL-17 and IL-22). When the 2 mutants were compared, vaccination with the SPI1 mutant proved to be more effective in the protection of poultry against S. Enteritidis challenge than the vaccination with the SPI2 mutant. On the other hand, vaccination with the SPI2 mutant stimulated a slightly higher antibody production and such a mutant might therefore be a better choice if Salmonella is used as a vector for the delivery of heterologous antigens with a desired stimulation of the humoral part of the immune system.
- MeSH
- aplikace orální MeSH
- atenuované vakcíny aplikace a dávkování genetika imunologie MeSH
- bakteriální nálož MeSH
- cytokiny sekrece MeSH
- genomové ostrovy MeSH
- kur domácí MeSH
- leukocyty mononukleární imunologie MeSH
- nemoci drůbeže imunologie prevence a kontrola MeSH
- protilátky bakteriální krev MeSH
- Salmonella enteritidis imunologie patogenita MeSH
- salmonelová infekce u zvířat imunologie mikrobiologie patologie prevence a kontrola MeSH
- salmonelové vakcíny aplikace a dávkování genetika imunologie MeSH
- sekundární imunizace metody MeSH
- slezina patologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
AIM: The occurrence and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in the environment of turkey farms in the Czech Republic were studied. METHODS AND RESULTS: Extended-spectrum beta-lactamase-producing E. coli isolates were found on 8 (20%) of 40 turkey farms surveyed. A total of 200 environmental smears were examined, and a total of 25 ESBL-producing E. coli were isolated. These isolates were analysed using XbaI pulsed-field gel electrophoresis and divided into nine pulsotypes. Most of the isolates harboured the gene bla(SHV-12) on a 40-kb plasmid of the IncFII group with an identical EcoRV restriction profile. Indistinguishable or clonally related SHV-12-producing isolates belonging to the same pulsotypes were found at some unrelated farms. CONCLUSIONS: Widespread occurrence of ESBL-producing E. coli isolates with bla(SHV-12) carried on IncFII plasmids in meat production flocks in the Czech Republic was demonstrated. SIGNIFICANCE AND IMPACT OF THE STUDY: Results indicate vertical transmission of ESBL-producing E. coli within the turkey production pyramid. The study shows the risk of multiresistant ESBL-producing bacteria and antibiotic-resistance genes being transmitted to humans via the food chain.
- MeSH
- beta-laktamasy genetika MeSH
- chov zvířat MeSH
- Escherichia coli enzymologie genetika izolace a purifikace MeSH
- infekce vyvolané Escherichia coli epidemiologie genetika mikrobiologie MeSH
- krocani MeSH
- lidé MeSH
- maso mikrobiologie MeSH
- plazmidy MeSH
- polymerázová řetězová reakce MeSH
- proteiny z Escherichia coli genetika MeSH
- pulzní gelová elektroforéza MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- bolesti na hrudi etiologie farmakoterapie MeSH
- bronchiální astma diagnóza etiologie MeSH
- gastroezofageální reflux diagnóza farmakoterapie komplikace MeSH
- inhibitory protonové pumpy MeSH
- kašel etiologie farmakoterapie MeSH
- lidé MeSH
- longitudinální studie MeSH
- protonové pumpy MeSH
- Check Tag
- lidé MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- akční potenciály MeSH
- dospělí MeSH
- interferon alfa terapeutické užití MeSH
- interferon beta terapeutické užití MeSH
- lidé MeSH
- myasthenia gravis diagnóza etiologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Streptococcus suis je na celom svete rozšírený zvierací patogén, vyvolávajúci rôzne závažné ochorenia najmä u prasiat. Od konca 60. rokov sú referované prípady humánnych, prevažne profesionálnych infekcií. V kazuistike rozoberáme zvláštnosti prvého popísaného prípadu meningitídy zapríčinenej Streptococcus suis typ 2 na Slovensku. Túto zoonózu sme diagnostikovali u 53ročného agronóma, ktorý sa poranil pri zabíjačke. Ochorenie sa vyznačovalo ťažkým, protrahovaným priebehom napriek adekvátnej antibiotickej liečbe. Pomcha sluchu a vestibulámeho aparátu, ktoré sú charakteristické a popisované v literatúre, sme pozorovali aj u nášho pacienta. Taktiež sme zaznamenali v literatúre citované problémy s etiologickou diagnostikou, ktoré sú príčinou toho, že ochorenie sa nemusí diagnostikovať u ludí v krajinách, kde je výskyt Streptococcus suis bežný.
Streptococcus suis is a worldwide spread, animal pathogen that provokes a number of serious diseases, especially in swine. Since the end of 1960's, cases of human (mostlv professional) infections have been referred to. A discussion of the character of the first case of meningitis caused by Streptococcus suis type 2 reported in Slovakia is presented in the case report. This zoonose was diagnosed in a 53-year-old agronomist who injured himself during a pig-slaughtering. The disease was characterized by a serious, protracted course in spite of an adequate treatment with antibiotics. A hearing defect and disorders in a function of the vestibular apparatus, that is normally described in the literature and is considered to be characteristic of the disease, was observed. The problems conceming the etiologic diagnosis described in the literature were also encountered. They are responsible for the fact that the disease can be difficult to diagnose in human individuals in the countries where Streptococcus suis commonly occurs.
- MeSH
- antibakteriální látky terapeutické užití MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- meningitida bakteriální diagnóza etiologie terapie MeSH
- Streptococcus suis izolace a purifikace MeSH
- zoonózy MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
