Brain edema is a fatal pathological state in which brain volume increases as a result of abnormal accumulation of fluid within the brain parenchyma. A key attribute of experimentally induced brain edema - increased brain water content (BWC) - needs to be verified. Various methods are used for this purpose: specific gravimetric technique, electron microscopic examination, magnetic resonance imaging (MRI) and dry/wet weight measurement. In this study, the cohort of 40 rats was divided into one control group (CG) and four experimental groups with 8 rats in each group. The procedure for determining BWC using dry/wet weight measurement was initiated 24 h after the completion of edema induction by the water intoxication method (WI group); after the intraperitoneal administration of Methylprednisolone (MP) together with distilled water during edema induction (WI+MP group); 30 min after osmotic blood brain barrier disruption (BBBd group); after injection of MP via the internal carotid artery immediately after BBBd (BBBd + MP group). While induction of brain edema (WI, BBBd) resulted in significantly higher BWC, there was no increase in BWC in the MP groups (WI+MP, BBBd+MP), suggesting a neuroprotective effect of MP in the development of brain edema.

• MeSH
• edém mozku * chemicky indukované   diagnostické zobrazování   patologie   MeSH
• edém patologie   MeSH
• hematoencefalická bariéra MeSH
• krysa rodu Rattus MeSH
• methylprednisolon farmakologie   MeSH
• mozek MeSH
• voda MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• methylprednisolon MeSH
• voda MeSH

Brain edema - a frequently fatal pathological state in which brain volume increases resulting in intracranial pressure elevation - can result from almost any insult to the brain, including traumatic brain injury. For many years, the objective of experimental studies was to find a method to prevent the development of brain edema at the onset. From this perspective, the use of methylprednisolone (MP) appears promising. High molecular MP (MW>50 kDa) can be incorporated into the brain - in the conditions of the experimental model - either by osmotic blood-brain barrier disruption (BBBd) or during the induction of cellular edema by water intoxication (WI) - a condition that increases the BBB permeability. The time window for administration of the MP should be at the earliest stages of edema. The neuroprotective effect of MP on the permeability of cytoplasmatic membranes of neuronal populations was proved. MP was administrated in three alternative ways: intraperitoneally during the induction of cytotoxic edema or immediately after finishing cytotoxic edema induction in a dose of 100 mg/kg b.w.; into the internal carotid artery within 2 h after finishing cytotoxic edema induction in a dose of 50 mg/kg b.w.; into internal carotid artery 10 min after edema induction by BBBd in a dose of 50 mg/kg b.w.

• MeSH
• edém mozku farmakoterapie   metabolismus   patologie   MeSH
• glukokortikoidy farmakologie   MeSH
• hematoencefalická bariéra účinky léků   metabolismus   patologie   MeSH
• kapilární permeabilita účinky léků   MeSH
• krysa rodu Rattus MeSH
• methylprednisolon farmakologie   MeSH
• modely nemocí na zvířatech MeSH
• mozek účinky léků   metabolismus   patologie   MeSH
• neurony účinky léků   metabolismus   patologie   MeSH
• neuroprotektivní látky farmakologie   MeSH
• potkani Wistar MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• glukokortikoidy MeSH
• methylprednisolon MeSH
• neuroprotektivní látky MeSH

• MeSH
• ANCA-asociované vaskulitidy diagnóza   farmakoterapie   MeSH
• aortální chlopeň diagnostické zobrazování   chirurgie   MeSH
• aortální insuficience diagnostické zobrazování   chirurgie   MeSH
• aplikace orální MeSH
• dítě MeSH
• lidé MeSH
• methylprednisolon aplikace a dávkování   terapeutické užití   MeSH
• počítačová rentgenová tomografie metody   MeSH
• prednison aplikace a dávkování   terapeutické užití   MeSH
• rituximab aplikace a dávkování   terapeutické užití   MeSH
• výsledek terapie MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH
• Názvy látek
• methylprednisolon MeSH
• prednison MeSH
• rituximab MeSH

Magnetic resonance imaging has been used for evaluating of a brain edema in experimental animals to assess cytotoxic and vasogenic edema by the apparent diffusion coefficient (ADC) and T2 imaging. This paper brings information about the effectiveness of methylprednisolone (MP) on experimental brain edema. A total of 24 rats were divided into three groups of 8 animals each. Rats with cytotoxic/intracellular brain edema induced by water intoxication were assigned to the group WI. These rats also served as the additional control group CG when measured before the induction of edema. A third group (WIMP) was intraperitoneally administered with methylprednisolone 100 mg/kg during water intoxication treatment. The group WI+MP was injected with methylprednisolone 50 mg/kg into the carotid artery within two hours after the water intoxication treatment. We evaluated the results in four groups. Two control groups (CG, WI) and two experimental groups (WIMP, WI+MP). Rats were subjected to MR scanning 24 h after edema induction. We observed significantly increased ADC values in group WI in both evaluated areas - cortex and hippocampus, which proved the occurrence of experimental vasogenic edema, while ADC values in groups WIMP and WI+MP were not increased, indicating that the experimental edema was not developed and thus confirming the protective effect of MP.

• MeSH
• antiflogistika farmakologie   MeSH
• edém mozku diagnostické zobrazování   farmakoterapie   patologie   MeSH
• hipokampus diagnostické zobrazování   účinky léků   MeSH
• krysa rodu Rattus MeSH
• magnetická rezonanční tomografie metody   MeSH
• methylprednisolon farmakologie   MeSH
• modely nemocí na zvířatech MeSH
• mozek diagnostické zobrazování   účinky léků   MeSH
• mozková kůra diagnostické zobrazování   účinky léků   MeSH
• potkani Wistar MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antiflogistika MeSH
• methylprednisolon MeSH

We report the case of a patient suffering from duplicity of myotonic dystrophy type 1 and ulcerative colitis whose treatment for ulcerative colitis included repeated administrations of descending doses of methylprednisolone and in whom we found an association between methylprednisolone dosing and cessation of myotonia. Myotonia severity was expressed as relaxation time after voluntary contraction and as a patient-reported outcome using the Czech version of the Myotonia Behavior Scale. The patient was being treated for a flare of ulcerative colitis, starting with 32 mg of methylprednisolone and reducing the dose by 4 mg a week. The symptoms of myotonia began to wear off three weeks after starting methylprednisolone and had totally disappeared by four weeks after starting methylprednisolone. The first symptoms of myotonia returned about a month after the last dose of methylprednisolone and reached a peak of severity more than two months after the final dose.

• Klíčová slova
• Corticosteroids, Methylprednisolone, Muscle relaxation, Myotonia, Myotonic dystrophy, Therapy,
• MeSH
• dospělí MeSH
• hormony kůry nadledvin aplikace a dávkování   MeSH
• lidé MeSH
• methylprednisolon aplikace a dávkování   MeSH
• myotonická dystrofie farmakoterapie   MeSH
• myotonie farmakoterapie   MeSH
• ulcerózní kolitida farmakoterapie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH
• Názvy látek
• hormony kůry nadledvin MeSH
• methylprednisolon MeSH

BACKGROUND: During the coronavirus disease (COVID-19) pandemic, people volunteered for sewing hand-made face masks. However, sewing-machine operating might be associated with high ergonomic risk and a negative impact on musculoskeletal health. OBJECTIVE AND METHODS: This paper describes an ultrasonographic diagnosis of a foot ganglion - after sewing 300 face masks within two months using a foot-operated sewing machine. RESULTS: The patient significantly improved after an ultrasound-guided aspiration and corticosteroid injection. CONCLUSION: In short, we highlight the importance of ultrasound examination in the management of work (overuse)-related disorders in occupational medicine practice.

• Klíčová slova
• Coronavirus, mask, seamstress, sewing machine, ultrasound,
• MeSH
• anestetika lokální aplikace a dávkování   MeSH
• antiflogistika aplikace a dávkování   MeSH
• COVID-19 * epidemiologie   prevence a kontrola   MeSH
• cystická ganglia diagnostické zobrazování   etiologie   MeSH
• dobrovolní pracovníci * MeSH
• drenáž metody   MeSH
• intervenční ultrasonografie MeSH
• lidé středního věku MeSH
• lidé MeSH
• masky MeSH
• methylprednisolon acetát aplikace a dávkování   MeSH
• nemoci nohy (od hlezna dolů) diagnostické zobrazování   etiologie   MeSH
• nemoci z povolání diagnostické zobrazování   etiologie   MeSH
• pandemie MeSH
• poranění nohy (od hlezna dolů) komplikace   MeSH
• poranění z opakovaného přetěžování komplikace   MeSH
• SARS-CoV-2 MeSH
• textilní průmysl * MeSH
• trimekain aplikace a dávkování   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• Názvy látek
• anestetika lokální MeSH
• antiflogistika MeSH
• methylprednisolon acetát MeSH
• trimekain MeSH

BACKGROUND: There is no universally accepted opinion on the use of granulocyte transfusions collected using apheresis (GTA) in neutropenic patients and severe infection. PATIENTS AND METHODS: The efficacy and safety of GTAs transfused at a single center over 10 years were analyzed retrospectively. GTAs were harvested from voluntary unrelated donors after priming with methylprednisolone using continuous apheresis and hydroxyethylstarch as sedimentation agent. RESULTS: 41 patients with neutropenia and hematologic malignancy (15 females and 26 males aged 22-69 (median 45.5)) were given a median 3.5 GTAs per patient (range: 1-17) containing a median 1.39×1010 granulocyte/GTA (range: 0.65-2.81). The indications for GTA use were soft tissue inflammation, sepsis, and pneumonia in 30, 22, and 14 cases, respectively. After GTA complete (30 patients: 73.2%) or partial (6 patients: 14.6%) healing of the infection was achieved. The success rate was 91.7% in soft tissue infections, 66.7% in invasive fungal infections, and 68% in sepsis. Septic shock (documented in 12 cases) was associated with a poor response (P<0.03; Chi-square test). Clinical worsening was observed in six cases (14.6%); four patients died. No significant short-term side effects of GTA treatment were recorded. CONCLUSIONS: In our study GTAs collected after steroid priming and used for the treatment of infection during severe neutropenia have shown comparable efficacy with several previously reported trials. However retrospective fashion of our study and inhomogeneous group of patients do not allow any firm conclusions. Prospective studies (including patients' registries) are needed for the better clarification of the role and the dose of GTAs necessary for the successful infection management during neutropenia.

• Klíčová slova
• Granulocyte from apheresis, Granulocytes de l’aphérèse, Infection sévère, Neutropénie sévère prolongée, Prolonged severe neutropenia, Severe infection,
• MeSH
• antiinfekční látky terapeutické užití   MeSH
• deriváty hydroxyethylového škrobu farmakologie   MeSH
• dospělí MeSH
• febrilní neutropenie komplikace   MeSH
• granulocyty transplantace   MeSH
• hematologické nádory komplikace   terapie   MeSH
• infekce měkkých tkání farmakoterapie   etiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• methylprednisolon farmakologie   terapeutické užití   MeSH
• mladý dospělý MeSH
• mykózy farmakoterapie   etiologie   MeSH
• pneumonie farmakoterapie   etiologie   MeSH
• převod jednotlivých krevních složek * MeSH
• retrospektivní studie MeSH
• senioři MeSH
• separace krevních složek MeSH
• sepse farmakoterapie   etiologie   MeSH
• transplantace hematopoetických kmenových buněk * MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antiinfekční látky MeSH
• deriváty hydroxyethylového škrobu MeSH
• methylprednisolon MeSH

Our aim was to compare the protective efficacy of two different formulations of methylprednisolone in T-2 toxin-induced cardiomyopathy. Methylprednisolone (soluble form, Lemod-solu® and/or depot form, Lemod-depo®, a total single dose of 40 mg/kg im) was given immediately after T-2 toxin (1 LD50 0.23 mg/kg sc). The myocardial tissue samples were examinated by using histopathology, semiquantitative and imaging analyses on day 1, 7, 14, 21, 28 and 60 of the study. Therapeutic application of Lemod-solu® significantly decreased the intensity of myocardial degeneration and haemorrhages, distribution of glycogen granules in the endo- and perimysium, a total number of mast cells and the degree of their degranulation was in correlation with the reversible heart structural lesions (p < 0.01 vs. T-2 toxin). These changes were completely abolished by the therapeutic use of Lemod-solu® plus Lemod-depo® (p < 0.001 vs. T-2 toxin). Our results show that a significant cardioprotective efficacy of methylprednisolone is mediated by its anti-inflammatory activity.

• Klíčová slova
• Cardiotoxicity, Methylprednisolone, Pathohistology, Rats, T-2 toxin,
• MeSH
• antiflogistika aplikace a dávkování   terapeutické užití   MeSH
• glykogen metabolismus   MeSH
• kardiomyopatie chemicky indukované   farmakoterapie   MeSH
• léky s prodlouženým účinkem MeSH
• mastocyty účinky léků   metabolismus   patologie   MeSH
• methylprednisolon aplikace a dávkování   terapeutické užití   MeSH
• myokard metabolismus   patologie   MeSH
• potkani Wistar MeSH
• T-2 toxin toxicita   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antiflogistika MeSH
• glykogen MeSH
• léky s prodlouženým účinkem MeSH
• methylprednisolon MeSH
• T-2 toxin MeSH

BACKGROUND: Variable effects of steroid minimization strategies on blood pressure in pediatric renal transplant recipients have been reported, but data on the effect of steroid withdrawal on ambulatory blood pressure and circadian blood pressure rhythm have not been published so far. METHODS: In a prospective, randomized, multicenter study on steroid withdrawal in pediatric renal transplant recipients (n = 42) on cyclosporine, mycophenolate mofetil, and methylprednisolone, we performed a substudy in 28 patients, aged 11.2 ± 3.8 years, for whom ambulatory blood pressure monitoring (ABPM) data were available. RESULTS: In the steroid-withdrawal group, the percentage of patients with arterial hypertension, defined as systolic and/or diastolic blood pressure values recorded by ABPM > 1.64 SDS and/or antihypertensive medication, at month 15 was significantly lower (35.7%, p = 0.002) than in controls (92.9%). The need of antihypertensive medication dropped significantly by 61.2% (p < 0.000 vs. control), while in controls, it even rose by 69.3%. One year after steroid withdrawal, no patient exhibited hypertensive blood pressure values above the 95th percentile, compared to 35.7% at baseline (p = 0.014) and to 14.3% of control (p = 0.142). The beneficial impact of steroid withdrawal was especially pronounced for nocturnal blood pressure, leading to a recovered circadian rhythm in 71.4% of patients vs. 14.3% at baseline (p = 0.002), while the percentage of controls with an abnormal circadian rhythm (35.7%) did not change. CONCLUSIONS: Steroid withdrawal in pediatric renal transplant recipients with well-preserved allograft function is associated with less arterial hypertension recorded by ABPM and recovery of circadian blood pressure rhythm by restoration of nocturnal blood pressure dipping.

• Klíčová slova
• Ambulatory blood pressure, Nocturnal blood pressure dipping, Pediatric renal transplantation, Randomized controlled trial, Steroid withdrawal,
• MeSH
• alografty imunologie   patofyziologie   MeSH
• ambulantní monitorování krevního tlaku MeSH
• cirkadiánní rytmus fyziologie   MeSH
• cyklosporin aplikace a dávkování   škodlivé účinky   MeSH
• dítě MeSH
• glukokortikoidy aplikace a dávkování   škodlivé účinky   MeSH
• hodnoty glomerulární filtrace fyziologie   MeSH
• homologní transplantace škodlivé účinky   MeSH
• hypertenze chemicky indukované   diagnóza   prevence a kontrola   MeSH
• imunosupresiva aplikace a dávkování   škodlivé účinky   MeSH
• krevní tlak účinky léků   MeSH
• kyselina mykofenolová aplikace a dávkování   škodlivé účinky   MeSH
• ledviny imunologie   patofyziologie   MeSH
• lidé MeSH
• methylprednisolon MeSH
• mladiství MeSH
• nenasazení léčby * MeSH
• prospektivní studie MeSH
• rejekce štěpu imunologie   patofyziologie   prevence a kontrola   MeSH
• transplantace ledvin škodlivé účinky   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• cyklosporin MeSH
• glukokortikoidy MeSH
• imunosupresiva MeSH
• kyselina mykofenolová MeSH
• methylprednisolon MeSH

OBJECTIVE: Epiduroscopy is a proven method of diagnosis and treatment for chronic radicular pain after spinal surgery, which is known as failed back surgery syndrome (FBSS). The aim of the study was to compare the efficacy of drugs (the enzyme hyaluronidase and corticosteroid DEPO-Medrol) administrated into the epidural space during epiduroscopy, performed within the ventral and ventro-lateral epidural space with a focus on releasing foraminal adhesions. METHODS: Forty-eight patients with diagnosed FBBS were randomized into two groups before epiduroscopy. Group A received the standard treatment-mechanical lysis of fibrotic tissue in the epidural space. Group B received hyaluronidase and corticosteroid methylprednisolone acetate during the procedure. Subjects were followed for six and 12 months via scheduled double-blinded examinations by pain physicians. Leg and back pain intensity was assessed by an 11-point numerical rating scale, and patients' functional disability was assessed by the Oswestry Disability Index (ODI). RESULTS: Study subjects showed a significant decrease in ODI score in both groups (P < 0.05). Significantly lower pain scores for leg pain (P < 0.05) and back pain (P < 0.05) were also recorded after the six-month follow-up. However, the one-year follow-up showed a return to the baseline ODI values of most monitored pain scores in both groups (P > 0.05). Improvement was only noted on the NRS for back pain at one-year follow-up (P < 0.05). CONCLUSIONS: A significant improvement of leg and back pain was found in both groups after six months. ODI was significantly improved only in group B in both the six- and 12-month intervals. Back pain at one-year follow-up was only improved in group B.

• Klíčová slova
• Adhesiolysis, Back Pain, Epiduroscopy, Failed Back Surgery Syndrome, Hyaluronidase,
• MeSH
• adheze tkání farmakoterapie   chirurgie   MeSH
• dvojitá slepá metoda MeSH
• epidurální prostor chirurgie   MeSH
• failed back surgery syndrom farmakoterapie   chirurgie   MeSH
• hormony kůry nadledvin terapeutické užití   MeSH
• hyaluronoglukosaminidasa terapeutické užití   MeSH
• lidé MeSH
• methylprednisolon terapeutické užití   MeSH
• neuroendoskopie metody   MeSH
• pilotní projekty MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• multicentrická studie MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• hormony kůry nadledvin MeSH
• hyaluronoglukosaminidasa MeSH
• methylprednisolon MeSH