Taxonomy provides a general foundation for research on insects. Using stored product pest (SPP) arthropods as a model group, this article overviews the historical impacts of taxonomy on applied entomology. The article surveys the dynamics of historical descriptions of new species in various SPP taxa; the majority of all species (90%) were described prior to 1925, while the key pests were described prior to 1866. The review shows that process of describing new SPP species is not random but is influenced by following factors: (i) larger species tend to be described earlier than smaller and SPP moths and beetles are described earlier than psocids and mites; (ii) key economic pests are on average described earlier than less significant ones. Considering a species name as a "password" to unique information resources, this review also assesses the historical number of synonymous or duplicate names of SPP species. Pests belonging to some higher taxa Lepidoptera and Coleoptera has accumulated more scientific synonyms than those others belonging to Psocoptera and Acari. Number of synonyms positively correlated with the economic importance of SPP species. The review summarized semantic origin of SPP names showing minor proportion of names (17.6%) are toponyms (geography) or eponyms (people), while the majority (82.4%) fall into other categories (descriptive, etc.). It is concluded that awareness of taxonomic advances, including changes to species and higher taxa names, should be effectively communicated to pest control practitioners and applied entomology students, and specifically addressed in relevant textbooks, web media, and databases.

• Klíčová slova
• Acari, insects, stored commodities, synonyms, taxonomy,
• MeSH
• entomologie * dějiny   MeSH
• hmyz * klasifikace   MeSH
• klasifikace * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

Psyllids (Hemiptera: Psylloidea) are plant sap-sucking insects whose identification is often difficult for non-experts. Despite the rapid development of DNA barcoding techniques and their widespread use, only a limited number of sequences of psyllids are available in the public databases, and those that are available are often misidentified. Here, we provide 80 sequences of two mitochondrial genes, cytochrome c oxidase I (COI) and cytochrome b (Cytb), for 25 species of Aphalaridae, mainly from Bulgaria. The DNA barcodes for 15 of these species are published for the first time. In cases where standard primers failed to amplify the target gene fragment, we designed new primers that can be used in future studies. The distance-based thresholds for the analysed species were between 0.0015 and 0.3415 for COI and 0.0771 and 0.4721 for Cytb, indicating that the Cytb gene has a higher interspecific divergence, compared to COI, and therefore allows for more accurate species identification. The species delimitation based on DNA barcodes is largely consistent with the differences resulting from morphological and host plant data, demonstrating that the use of DNA barcodes is suitable for successful identification of most aphalarid species studied. The phylogenetic reconstruction based on maximum likelihood and Bayesian inference analyses, while showing similar results at high taxonomic levels to previously published phylogenies, provides additional information on the placement of aphalarids at the species level. The following five species represent new records for Bulgaria: Agonoscena targionii, Aphalara affinis, Colposcenia aliena, Co. bidentata, and Craspedolepta malachitica. Craspedolepta conspersa is reported for the first time from the Czech Republic, while Agonoscena cisti is reported for the first time from Albania.

• Klíčová slova
• DNA barcoding, distance-based method, phylogeny, psyllids, rapid species identification, sequence database,
• Publikační typ
• časopisecké články MeSH

The family Cimicidae comprises ectoparasites feeding exclusively on the blood of endothermic animals. Cimicid swallow bugs specifically target swallow birds (Hirundinidae) and their nestlings in infested nests. Bugs of the genus Oeciacus are commonly found in mud nests of swallows and martins, while they rarely visit the homes of humans. Although-unlike other cimicid species-the house martin bug Oeciacus hirundinis has never been reported as a vector of zoonotic pathogens, its possible role in arbovirus circulation in continental Europe is unclear. Samples of O. hirundinis were therefore collected from abandoned house martin (Delichon urbicum) nests in southern Moravia (Czech Republic) during the 2021/2022 winter season and checked for alpha-, flavi- and bunyaviruses by RT-PCR. Of a total of 96 pools consisting of three adult bugs each, one pool tested positive for Usutu virus (USUV)-RNA. Phylogenetic analysis showed that the virus strain was closely related to Italian and some Central European strains and corresponded to USUV lineage 5. The detection of USUV in O. hirundinis during wintertime in the absence of swallows raises the question for a possible role of this avian ectoparasite in virus overwintering in Europe.

• Klíčová slova
• Hirundo rustica, Oeciacus hirundinis, Cimicidae, House martin, Overwintering, Usutu virus,
• MeSH
• Cimicidae * virologie   MeSH
• Flavivirus * izolace a purifikace   genetika   klasifikace   MeSH
• fylogeneze * MeSH
• nemoci ptáků parazitologie   virologie   MeSH
• RNA virová genetika   MeSH
• roční období * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• RNA virová MeSH

Assessments of biodiversity and ecosystem status can benefit from DNA metabarcoding as a means to streamline sample processing and specimen identification. Moreover, processing the fixation medium instead of the precious material introduces straightforward protocols that allow subsequent focus on certain organisms detected among the preserved specimens. In this study, we present a proof of concept via the analysis of freshwater invertebrate samples from the Tatra Mountain lakes (Slovakia). Besides highlighting a match between the lake-specific environmental conditions and the results of our fixative DNA metabarcoding, we observed an option to fine-tune the fixation time: to prefer two weeks over a day or a month. This effect emerged from the presence/absence of individual taxa rather than from coarse per-sample records of taxonomic richness, demonstrating that metabarcoding studies-and efforts to optimize their protocols-can use the robust metrics to explore even subtle trends. We also provide evidence that fixative DNA might better capture large freshwater species than terrestrial or meiofauna.

• MeSH
• bezobratlí genetika   klasifikace   MeSH
• biodiverzita * MeSH
• DNA genetika   izolace a purifikace   analýza   MeSH
• ekosystém MeSH
• jezera * MeSH
• taxonomické DNA čárové kódování * metody   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• DNA MeSH

The Palaearctic species of Aphodius Hellwig, 1798, subgenus Liothorax Motschulsky, 1860 are revised using a combination of chromosome analysis, molecular phylogenetics and morphological statistical analysis. Sixteen species are recognised, one of which is shown to comprise two subspecies. Based mainly on the morphology of the aedeagal endophallus and the phylogenetic analysis, they are placed in two groups: the. niger group, apparently monophyletic, comprising Aphodius (Liothorax) niger Illiger, 1798, A. (L.) muscorum (Ádám, 1994), stat. rest., A. (L.) felixsp. nov., A. (L.) bellumgerenssp. nov., A. (L.) bameulisp. nov., A. (L.) krellisp. nov., A. (L.) isikdagensis (Balthasar, 1953), A. (L.) albertisp. nov. and A. (L.) wilsonae Maté et Angus, 2005, stat. rest.; and the plagiatus group, almost certainly paraphyletic, comprising A. (L.) plagiatus (Linnaeus, 1767), including A. (L.) p. plagiatus and A. (L.) p. sinoplagiatussubsp. nov., A. (L.) rodrigoisp. nov., A. (L.) discoidesA. Schmidt, 1916, stat. rest., A. (L.) rutilipennis (Baudi di Selve, 1870), stat. rest., A. (L.) chellalasp. nov., A. (L.) kraatzi Harold, 1868, and A. (L.) rusakovi Gusakov, 2004. A key to the species is given as well as details of their morphology, distributions, and habitats.

• Klíčová slova
• Chromosomes, Scarabaeidae, distribution, habitats, morphology, mtDNA, new species, taxonomy,
• Publikační typ
• časopisecké články MeSH

Environmental DNA (eDNA) metabarcoding has gained growing attention as a strategy for monitoring biodiversity in ecology. However, taxa identifications produced through metabarcoding require sophisticated processing of high-throughput sequencing data from taxonomically informative DNA barcodes. Various sets of universal and taxon-specific primers have been developed, extending the usability of metabarcoding across archaea, bacteria and eukaryotes. Accordingly, a multitude of metabarcoding data analysis tools and pipelines have also been developed. Often, several developed workflows are designed to process the same amplicon sequencing data, making it somewhat puzzling to choose one among the plethora of existing pipelines. However, each pipeline has its own specific philosophy, strengths and limitations, which should be considered depending on the aims of any specific study, as well as the bioinformatics expertise of the user. In this review, we outline the input data requirements, supported operating systems and particular attributes of thirty-two amplicon processing pipelines with the goal of helping users to select a pipeline for their metabarcoding projects.

• Klíčová slova
• amplicon data analysis, bioinformatics, environmental DNA, metabarcoding, pipeline, review,
• MeSH
• analýza dat MeSH
• Archaea genetika   klasifikace   MeSH
• Bacteria genetika   klasifikace   MeSH
• environmentální DNA genetika   MeSH
• Eukaryota genetika   klasifikace   MeSH
• metagenomika metody   MeSH
• software * MeSH
• taxonomické DNA čárové kódování * metody   MeSH
• výpočetní biologie * metody   MeSH
• vysoce účinné nukleotidové sekvenování metody   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• environmentální DNA MeSH

Global biodiversity gradients are generally expected to reflect greater species replacement closer to the equator. However, empirical validation of global biodiversity gradients largely relies on vertebrates, plants, and other less diverse taxa. Here we assess the temporal and spatial dynamics of global arthropod biodiversity dynamics using a beta-diversity framework. Sampling includes 129 sampling sites whereby malaise traps are deployed to monitor temporal changes in arthropod communities. Overall, we encountered more than 150,000 unique barcode index numbers (BINs) (i.e. species proxies). We assess between site differences in community diversity using beta-diversity and the partitioned components of species replacement and richness difference. Global total beta-diversity (dissimilarity) increases with decreasing latitude, greater spatial distance and greater temporal distance. Species replacement and richness difference patterns vary across biogeographic regions. Our findings support long-standing, general expectations of global biodiversity patterns. However, we also show that the underlying processes driving patterns may be regionally linked.

• MeSH
• biodiverzita * MeSH
• časoprostorová analýza MeSH
• členovci * klasifikace   fyziologie   MeSH
• zeměpis MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The study presents DNA barcoding results of five families of Hymenoptera in Germany. DNA barcodes are provided for 24 of the 25 species of Gasteruption occurring in Central Europe, including 18 of the 19 species recorded from Germany. The genetic diversity was higher than expected, with five species exhibiting two or more Barcode Index Number (BINs), whereas BIN sharing occurred in four species. Gasteruptionfoveiceps Semenov, 1892, stat. nov. is removed from synonymy with G.nigrescens Schletterer, 1885 and treated as a distinct species.

• Klíčová slova
• COI, Central Europe, DNA barcoding, insects, morphology, taxonomy,
• Publikační typ
• časopisecké články MeSH

Food adulteration is one of the most serious problems regarding food safety and quality worldwide. Besides misleading consumers, it poses a considerable health risk associated with the potential non-labeled allergen content. Fish and fish products are one of the most expensive and widely traded commodities, which predisposes them to being adulterated. Among all fraud types, replacing high-quality or rare fish with a less valuable species predominates. Because fish differ in their allergen content, specifically the main one, parvalbumin, their replacement can endanger consumers. This underlines the need for reliable, robust control systems for fish species identification. Various methods may be used for the aforementioned purpose. DNA-based methods are favored due to the characteristics of the target molecule, DNA, which is heat resistant, and the fact that through its sequencing, several other traits, including the recognition of genetic modifications, can be determined. Thus, they are considered to be powerful tools for identifying cases of food fraud. In this review, the major DNA-based methods applicable for fish meat and product authentication and their commercial applications are discussed, the possibilities of detecting genetic modifications in fish are evaluated, and future trends are highlighted, emphasizing the need for comprehensive and regularly updated online database resources.

• Klíčová slova
• DNA-based methods, fish, food fraud, food quality, genetically modified organism (GMO), polymerase chain reaction (PCR), species identification,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

The elateroid family Lycidae is known for limited dispersal propensity and high species-level endemism. The red net-winged beetle, Dictyoptera aurora (Herbst, 1874), differs from all relatives by the range comprising almost the entire Holarctic region. Based on a five-marker phylogeny and 67 barcode entries (cox1-5' mtDNA) from the whole range, we recovered two genetically distinct species within traditionally defined D. aurora and resurrected the name D. coccinata (Say, 1835) as the oldest available synonym for Nearctic populations. Yet, no reliable morphological trait distinguishes these species except for minute differences in the male genitalia. D. coccinata is a monophylum resulting from a single Miocene dispersal event, ~15.8 million years ago, and genetic divergence implies long-term isolation by the Bering Strait. Far East Asian and west European populations are also genetically distinct, although to a lower extent. Two independent colonization events established the Fennoscandian populations after the last glacial maximum. Besides intrinsic factors, the high morphological similarity might result from stabilizing selection for shared aposematic signals. The rapidly accumulating barcode data provide valuable information on the evolutionary history and the origins of regional faunas.

• Klíčová slova
• Müllerian mimicry, aposematic signal, barcode, cryptic species, dispersal, last glacial maximum, morphological stasis, mtDNA, rRNA, taxonomy,
• Publikační typ
• časopisecké články MeSH