BACKGROUND: Psoriasis is a chronic systemic inflammatory disease associated with a wide range of comorbidities, including metabolic syndrome (MetS). Serum calprotectin, ANGPTL8, and oxidative damage to nucleic acids might be associated with both diseases. The presented study describes the influence of psoriasis and MetS on the serum levels of markers of systemic inflammation (calprotectin and ANGPTL8) and markers of oxidative damage to nucleic acids. The applicability of serum levels of calprotectin and ANGPTL8 for monitoring of the activity of psoriasis (diagnostic markers) is also evaluated. METHODS: Clinical examination (PASI score, MetS), enzyme-linked immunosorbent assay (ELISA), and Enzyme Immunoassay (EIA). Serum calprotectin, ANGPTL8, 8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanosine, and 8-hydroxyguanine. Results and Conclusions. The psoriasis significantly increased the serum level of calprotectin and the serum level of oxidative damage to nucleic acids, however not the serum level of ANGPTL8. The presence of MetS did not significantly affect the serum levels of calprotectin, ANGPTL8, and oxidative damage to nucleic acids in either psoriasis patients or controls. It seems that the serum level of calprotectin (but not the serum level of ANGPTL8) could be used as a biomarker for monitoring the activity of psoriasis.

• MeSH
• angiopoetinu podobné proteiny krev   MeSH
• angiopoetinu podobný protein 8 MeSH
• biologické markery krev   MeSH
• dospělí MeSH
• leukocytární L1-antigenní komplex krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• metabolický syndrom diagnóza   MeSH
• mladiství MeSH
• mladý dospělý MeSH
• nukleové kyseliny metabolismus   MeSH
• oxidační stres MeSH
• peptidové hormony krev   MeSH
• poškození DNA MeSH
• psoriáza diagnóza   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• zánět diagnóza   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• angiopoetinu podobné proteiny MeSH
• angiopoetinu podobný protein 8 MeSH
• ANGPTL8 protein, human MeSH Prohlížeč
• biologické markery MeSH
• leukocytární L1-antigenní komplex MeSH
• nukleové kyseliny MeSH
• peptidové hormony MeSH

BACKGROUND: Numerous articles related to S100 proteins have been recently published. This review aims to introduce this large protein family and its importance in the diagnostics of many pathological conditions in children and adults. DATA SOURCES: Based on original publications found in database systems, we summarize the current knowledge about the S100 protein group and highlight the most important proteins with focus on pediatric use. RESULTS: The S100 family is composed of Ca2+ and Zn2+ binding proteins, which are present only in vertebrates. Some of these proteins can be used as diagnostic markers in cardiology (S100A1, S100A12), oncology (S100A2, S100A5, S100A6, S100A14, S100A16, S100P, S100B), neurology (S100B), rheumatology (S100A8/A9, S100A4, S100A6, and S100A12), nephrology and infections (S100A8, S100A9, S100A8/A9, S100A12). The most useful S100 proteins in pediatrics are S100A8, S100A9, heterodimers S100A8/A9, S100B and S100A12. CONCLUSIONS: The S100 family members are promising biomarkers and provide numerous possibilities for implementation into clinical practice to optimize the differential diagnostic process.

• Klíčová slova
• Diagnostics, Marker, Pediatrics, S100,
• MeSH
• biologické markery krev   MeSH
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé MeSH
• neonatologie * MeSH
• novorozenec MeSH
• pediatrie * MeSH
• prediktivní hodnota testů MeSH
• prognóza MeSH
• proteiny S100 krev   MeSH
• senzitivita a specificita MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• biologické markery MeSH
• proteiny S100 MeSH

BACKGROUND The aim of the study was to investigate the role of von Willebrand factor (vWF), the vWF-cleaving protease, ADAMTS13, the composition of thrombus, and patient outcome following mechanical cerebral artery thrombectomy in patients with acute ischemic stroke. MATERIAL AND METHODS A prospective cohort study included 131 patients with ischemic stroke (<6 hours) with or without intravenous thrombolysis. Interventional procedure parameters, hemocoagulation markers, vWF, ADAMTS13, and histological examination of the extracted thrombi were performed. The National Institutes of Health Stroke Scale (NIHSS) score was used on hospital admission, after 24 hours, at day 7; the three-month modified Rankin Scale score was used. RESULTS Mechanical thrombectomy resulted in a Treatment in Cerebral Ischemia (TICI) score of 2-3, with recanalization in 89% of patients. Intravenous thrombolysis was used in 101 (78%). Patients with and without intravenous thrombolysis therapy had a good clinical outcome (score 0-2) in 47% of cases (P=0.459) using the three-month modified Rankin Scale. Patients with a National Institutes of Health Stroke Scale (NIHSS) score ≥15 had significantly increased vWF levels (P=0.003), and a significantly increased vWF: ADAMTS13 ratio (P=0.038) on hospital admission. Significant correlation coefficients were found for plasma vWF and thrombo-embolus vWF (r=0.32), platelet (r=0.24), and fibrin (r=0.26) levels. In the removed thrombus, vWF levels were significantly correlated with platelet count (r=0.53), CD31-positive cells (r=0.38), and fibrin (r=0.48). CONCLUSIONS In patients with acute ischemic stroke, mechanical cerebral artery thrombectomy resulted in a good clinical outcome in 47% of cases, with and without intravenous thrombolysis therapy.

• MeSH
• arteriae cerebrales metabolismus   patologie   MeSH
• cévní mozková příhoda krev   metabolismus   chirurgie   MeSH
• demografie MeSH
• hemokoagulace MeSH
• ischemie mozku krev   metabolismus   chirurgie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• protein ADAMTS13 metabolismus   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• trombektomie * MeSH
• trombóza krev   imunologie   metabolismus   MeSH
• von Willebrandův faktor metabolismus   MeSH
• výsledek terapie MeSH
• zánět patologie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• ADAMTS13 protein, human MeSH Prohlížeč
• protein ADAMTS13 MeSH
• von Willebrandův faktor MeSH

BACKGROUND: The role of glucan in the stimulation of immune reactions is well established. In our report, we focused on the effects of orally administered glucan on nonspecific immunity of children with chronic respiratory problems. MATERIALS AND METHODS: We measured the levels of albumin, lysozyme. C-reactive protein (CRP) and calprotectin in the saliva of 60 children ages 8-12 years and evaluated the effects of 30-day treatment with 100 mg/d oral dose of glucan. RESULTS: We found a significant increase in the production of lysozyme, CRP and calprotectin in glucan-treated children. CONCLUSIONS: Short-term oral application of natural immunomodulator β-glucan significantly stimulated mucosal immunity of children with chronic respiratory problems.

• Klíčová slova
• Glucan, children, lysozyme, mucosal immunity, saliva,
• Publikační typ
• časopisecké články MeSH

Innate immunity is shaped by a complex of redundant and pleiotropic factors that ensure recognition, alert and suppression of pathogens. Innate immune responses in the gut are complicated by the requirement of parallel tolerance to commensal microflora predominating in cell numbers and species. In normal individuals, the intestinal mucosa together with relevant lymph nodes represents a robust barrier against systemic spread of non-typhoid Salmonella. Contemporary insights into these defense mechanisms are reviewed.

• MeSH
• gastrointestinální trakt imunologie   MeSH
• lidé MeSH
• přirozená imunita * MeSH
• Salmonella imunologie   MeSH
• salmonelóza imunologie   MeSH
• slizniční imunita * MeSH
• střevní sliznice imunologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

Probiotics modulate production of both cytokine and antimicrobial peptides. This effect can be regarded as a part of complex interplay between them and the host.

• MeSH
• Bifidobacterium imunologie   MeSH
• cytokiny metabolismus   MeSH
• kationické antimikrobiální peptidy metabolismus   MeSH
• Lactobacillus imunologie   MeSH
• lidé MeSH
• probiotika farmakologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• cytokiny MeSH
• kationické antimikrobiální peptidy MeSH

The pig amnion was in vivo intraamniotically infected with E. coli for 10 h at 80-85 d of gestation either with the nonpathogenic O86 strain or enteropathogenic O55 strain. TNF-alpha, IL-10, IL-1beta and IFN-gamma were determined in amniotic fluids by ELISA, the expression of cytokines and some other inflammatory markers was determined by immunohistochemistry. Intraamniotic infection induced high levels of TNF-alpha in amniotic fluids which correlated with bacterial virulence whereas IL-10 was induced only by O86. The IL-1beta level did not increase significantly and was expressed in all infected membranes. IFN-gamma was negligible or absent. TNF-alpha, IL-12p40, calprotectin, HSP65 and gp91phox were found by immunohistochemistry only in amnion membranes infected with the enteropathogenic strain 055.

• MeSH
• amnion imunologie   mikrobiologie   MeSH
• biologické markery metabolismus   MeSH
• cytokiny metabolismus   MeSH
• Escherichia coli imunologie   patogenita   MeSH
• infekce vyvolané Escherichia coli imunologie   mikrobiologie   veterinární   MeSH
• infekční komplikace v těhotenství imunologie   mikrobiologie   veterinární   MeSH
• interferon gama metabolismus   MeSH
• interleukin-1 metabolismus   MeSH
• interleukin-10 metabolismus   MeSH
• nemoci prasat imunologie   mikrobiologie   MeSH
• prasata MeSH
• těhotenství MeSH
• TNF-alfa metabolismus   MeSH
• zánět imunologie   MeSH
• zvířata MeSH
• Check Tag
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické markery MeSH
• cytokiny MeSH
• interferon gama MeSH
• interleukin-1 MeSH
• interleukin-10 MeSH
• TNF-alfa MeSH