Epigenetic regulation of gene expression is expected to be an important mechanism behind phenotypic plasticity. Whether epigenetic regulation affects species ecophysiological adaptations to changing climate remains largely unexplored. We compared ecophysiological traits between individuals treated with 5-azaC, assumed to lead to DNA demethylation, with control individuals of a clonal grass originating from and grown under different climates, simulating different directions and magnitudes of climate change. We linked the ecophysiological data to proxies of fitness. Main effects of plant origin and cultivating conditions predicted variation in plant traits, but 5-azaC did not. Effects of 5-azaC interacted with conditions of cultivation and plant origin. The direction of the 5-azaC effects suggests that DNA methylation does not reflect species long-term adaptations to climate of origin and species likely epigenetically adjusted to the conditions experienced during experiment set-up. Ecophysiology translated to proxies of fitness, but the intensity and direction of the relationships were context dependent and affected by 5-azaC. The study suggests that effects of DNA methylation depend on conditions of plant origin and current climate. Direction of 5-azaC effects suggests limited role of epigenetic modifications in long-term adaptation of plants. It rather facilitates fast adaptations to temporal fluctuations of the environment.

• MeSH
• Azacitidine pharmacology   MeSH
• Epigenesis, Genetic * MeSH
• Climate Change MeSH
• Humans MeSH
• DNA Methylation * MeSH
• Genes, Plant MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Azacitidine MeSH

Filing gaps in our understanding of species' abilities to adapt to novel climates is a key challenge for predicting future range shifts and biodiversity loss. Key knowledge gaps are related to the potential for evolutionary rescue in response to climate, especially in long-lived clonally reproducing species. We illustrate a novel approach to assess the potential for evolutionary rescue using a combination of reciprocal transplant experiment in the field to assess performance under a changing climate and independent growth chamber assays to assess growth- and physiology-related plant trait maxima and plasticities of the same clones. We use a clonal grass, Festuca rubra, as a model species. We propagated individual clones and used them in a transplant experiment across broad-scale temperature and precipitation gradients, simulating the projected direction of climate change in the region. Independent information on trait maxima and plasticities of the same clones was obtained by cultivating them in four growth chambers representing climate extremes. Plant survival was affected by interaction between plant traits and climate change, with both trait plasticities and maxima being important for adaptation to novel climates. Key traits include plasticity in extravaginal ramets, aboveground biomass, and osmotic potential. The direction of selection in response to a given climatic change detected in this study mostly contradicted the natural trait clines indicating that short-term selection pressure as identified here does not match long-term selection outcomes. Long-lived clonal species exposed to different climatic changes are subjected to consistent selection pressures on key traits, a necessary condition for adaptation to novel conditions. This points to evolutionary rescue as an important mechanism for dealing with climate change in these species. Our experimental approach may be applied also in other model systems broadening our understanding of evolutionary rescue. Such knowledge cannot be easily deduced from observing the existing field clines.

• Keywords
• alpine ecosystems, clonal species, rapid evolution, reciprocal transplant experiment, trait selection,
• Publication type
• Journal Article MeSH

Clonal plants in heterogeneous environments can benefit from their habitat selection behavior, which enables them to utilize patchily distributed resources efficiently. It has been shown that such behavior can be strongly influenced by their memories on past environmental interactions. Epigenetic variation such as DNA methylation was proposed to be one of the mechanisms involved in the memory. Here, we explored whether the experience with Ultraviolet B (UV-B) radiation triggers epigenetic memory and affects clonal plants' foraging behavior in an UV-B heterogeneous environment. Parental ramets of Glechoma longituba were exposed to UV-B radiation for 15 days or not (controls), and their offspring ramets were allowed to choose light environment enriched with UV-B or not (the species is monopodial and can only choose one environment). Sizes and epigenetic profiles (based on methylation-sensitive amplification polymorphism analysis) of parental and offspring plants from different environments were also analyzed. Parental ramets that have been exposed to UV-B radiation were smaller than ramets from control environment and produced less and smaller offspring ramets. Offspring ramets were placed more often into the control light environment (88.46% ramets) than to the UV-B light environment (11.54% ramets) when parental ramets were exposed to UV-B radiation, which is a manifestation of "escape strategy." Offspring of control parental ramets show similar preference to the two light environments. Parental ramets exposed to UV-B had lower levels of overall DNA methylation and had different epigenetic profiles than control parental ramets. The methylation of UV-B-stressed parental ramets was maintained among their offspring ramets, although the epigenetic differentiation was reduced after several asexual generations. The parental experience with the UV-B radiation strongly influenced foraging behavior. The memory on the previous environmental interaction enables clonal plants to better interact with a heterogeneous environment and the memory is at least partly based on heritable epigenetic variation.

• Keywords
• UV-B radiation, clonal plant, epigenetic memory, foraging behavior, habitat selection, heterogeneous environment,
• Publication type
• Journal Article MeSH

Knowledge of the relationship between environmental conditions and species traits is an important prerequisite for understanding determinants of community composition and predicting species response to novel climatic conditions. Despite increasing number of studies on this topic, our knowledge on importance of genetic differentiation, plasticity and their interactions along larger sets of species is still limited especially for traits related to plant ecophysiology. We studied variation in traits related to growth, leaf chemistry, contents of photosynthetic pigments and activity of antioxidative enzymes, stomata morphology and photosynthetic activity across eight Impatiens species growing along altitudinal gradients in Himalayas cultivated in three different temperature regimes and explored effects of among species phylogenetic relationships on the results. Original and target climatic conditions determine trait values in our system. The traits are either highly plastic (e.g., APX, CAT, plant size, neoxanthin, β-carotene, chlorophyll a/b, DEPSC) or are highly differentiated among populations (stomata density, lutein production). Many traits show strong among population differentiation in degree of plasticity and direction in response to environmental changes. Most traits indicate that the species will profit from the expected warming. This suggests that different processes determine the values of the different traits and separating the importance of genetic differentiation and plasticity is crucial for our ability to predict species response to future climate changes. The results also indicate that evolution of the traits is not phylogenetically constrained but including phylogenetic information into the analysis may improve our understanding of the trait-environment relationships as was apparent from the analysis of SLA.

• Keywords
• Balsaminaceae, antioxidants, carotenoids, elevational gradients, genotype × environment interaction, growth chamber experiment, phylogenetic constrains, xanthophyll cycle,
• Publication type
• Journal Article MeSH

Active foraging for patchy resources is a crucial feature of many clonal plant species. It has been recently shown that plants' foraging for resources can be facilitated by anticipatory behavior via association of resource position with other environmental cues. We therefore tested whether clones of Fragaria vesca are able to associate and memorize positions of soil nutrients with particular light intensity, which will consequently enable them anticipating nutrients in new environment. We trained clones of F. vesca for nutrients to occur either in shade or in light. Consequently, we tested their growth response to differing light intensity in the absence of soil nutrients. We also manipulated epigenetic status of a subset of the clones to test the role of DNA methylation in the anticipatory behavior. Clones of F. vesca were able to associate presence of nutrients with particular light intensity, which enabled them to anticipate nutrient positions in the new environment based on its light intensity. Clones that had been trained for nutrients to occur in shade increased placement of ramets to shade whereas clones trained for nutrients to occur in light increased biomass of ramets in light. Our study clearly shows that the clonal plant F. vesca is able to relate two environmental factors, light and soil nutrients, and use this connection in anticipatory behavior. We conclude that anticipatory behavior can substantially improve the ability of clonal plants to utilize scarce and unevenly distributed resources.

• Keywords
• 5-azacytidine, DNA methylation, anticipatory behavior, epigenetic variation, foraging, intelligence, light, nutrients,
• Publication type
• Journal Article MeSH

Transgenerational effects (TGE) can modify phenotypes of offspring generations playing thus a potentially important role in ecology and evolution of many plant species. These effects have been studied mostly across generations of sexually reproducing species. A substantial proportion of plant species are however reproducing asexually, for instance via clonal growth. TGE are thought to be enabled by heritable epigenetic modification of DNA, although unambiguous evidence is still scarce. On the clonal herb white clover (Trifolium repens), we tested the generality of clonal TGE across five genotypes and five parental environments including soil contamination and above-ground competition. Moreover, by genome wide-methylation variation analysis we explored the role of drought, one of the parental environments that triggered the strongest TGE. We tested the induction of epigenetic changes in offspring generations using several intensities and durations of drought stress. We found that TGE of different environments were highly genotype specific and all tested environments triggered TGE at least in some genotypes. In addition, parental drought stresses triggered epigenetic change in T. repens and most of the induced epigenetic change was maintained across several clonal offspring generations. We conclude that TGE are common and genotype specific in clonal plant T. repens and potentially under epigenetic control.

• Keywords
• T. repens, abiotic stress, clonal reproduction, plant memory, transgenerational effects,
• Publication type
• Journal Article MeSH

Species response to climate change is influenced by predictable (selective) and unpredictable (random) evolutionary processes. To understand how climate change will affect present-day species, it is necessary to assess their adaptive potential and distinguish it from the effects of random processes. This will allow predicting how different genotypes will respond to forecasted environmental change. Space for time substitution experiments are an elegant way to test the response of present day populations to climate variation in real time. Here we assess neutral and putatively adaptive variation in 11 populations of Festuca rubra situated along crossed gradients of temperature and moisture using molecular markers and phenotypic measurements, respectively. By comparing population differentiation in putatively neutral molecular markers and phenotypic traits (QST-FST comparisons), we show the existence of adaptive differentiation in phenotypic traits and their plasticity across the climatic gradient. The observed patterns of differentiation are due to the high genotypic and phenotypic differentiation of the populations from the coldest (and wettest) environment. Finally, we observe statistically significant covariation between markers and phenotypic traits, which is likely caused by isolation by adaptation. These results contribute to a better understanding of the current adaptation and evolutionary potential to face climate change of a widespread species. They can also be extrapolated to understand how the studied populations will adjust to upcoming climate change without going through the lengthy process of phenotyping.

• MeSH
• Phenotype MeSH
• Festuca physiology   MeSH
• Adaptation, Physiological MeSH
• Genotype MeSH
• Climate Change * MeSH
• Quantitative Trait, Heritable * MeSH
• Microsatellite Repeats genetics   MeSH
• Genetics, Population MeSH
• Selection, Genetic MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Phragmites australis is a cosmopolitan grass and often the dominant species in the ecosystems it inhabits. Due to high intraspecific diversity and phenotypic plasticity, P. australis has an extensive ecological amplitude and a great capacity to acclimate to adverse environmental conditions; it can therefore offer valuable insights into plant responses to global change. Here we review the ecology and ecophysiology of prominent P. australis lineages and their responses to multiple forms of global change. Key findings of our review are that: (1) P. australis lineages are well-adapted to regions of their phylogeographic origin and therefore respond differently to changes in climatic conditions such as temperature or atmospheric CO2; (2) each lineage consists of populations that may occur in geographically different habitats and contain multiple genotypes; (3) the phenotypic plasticity of functional and fitness-related traits of a genotype determine the responses to global change factors; (4) genotypes with high plasticity to environmental drivers may acclimate or even vastly expand their ranges, genotypes of medium plasticity must acclimate or experience range-shifts, and those with low plasticity may face local extinction; (5) responses to ancillary types of global change, like shifting levels of soil salinity, flooding, and drought, are not consistent within lineages and depend on adaptation of individual genotypes. These patterns suggest that the diverse lineages of P. australis will undergo intense selective pressure in the face of global change such that the distributions and interactions of co-occurring lineages, as well as those of genotypes within-lineages, are very likely to be altered. We propose that the strong latitudinal clines within and between P. australis lineages can be a useful tool for predicting plant responses to climate change in general and present a conceptual framework for using P. australis lineages to predict plant responses to global change and its consequences.

• Keywords
• atmospheric CO2, climate change, eutrophication, global distribution, intraspecific variation, invasive species, salinity, temperature,
• Publication type
• Journal Article MeSH
• Review MeSH