Objectives: To advance our knowledge of disease mechanisms and therapeutic options, understanding cell cycle regulation is critical. Recent research has highlighted the importance of reactive oxygen species (ROS) in cell cycle regulation. Although excessive ROS levels can lead to age-related pathologies, ROS also play an essential role in normal cellular functions. Many cell cycle regulatory proteins are affected by their redox status, but the precise mechanisms and conditions under which ROS promote or inhibit cell proliferation are not fully understood.Methods: This review presents data from the scientific literature and publicly available databases on changes in redox state during the cell cycle and their effects on key regulatory proteins.Results: We identified redox-sensitive targets within the cell cycle machinery and analysed different effects of ROS (type, concentration, duration of exposure) on cell cycle phases. For example, moderate levels of ROS can promote cell proliferation by activating signalling pathways involved in cell cycle progression, whereas excessive ROS levels can induce DNA damage and trigger cell cycle arrest or cell death.Discussion: Our findings encourage future research focused on identifying redox-sensitive targets in the cell cycle machinery, potentially leading to new treatments for diseases with dysregulated cell proliferation.
- Klíčová slova
- Cell cycle, cell cycle signaling, oxidative stress, proliferation, reactive oxygen species, redox state, redox-sensitive targets,
- MeSH
- buněčný cyklus * MeSH
- lidé MeSH
- oxidace-redukce * MeSH
- poškození DNA MeSH
- proliferace buněk MeSH
- reaktivní formy kyslíku * metabolismus MeSH
- signální transdukce MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- reaktivní formy kyslíku * MeSH
BACKGROUND: The study was undertaken to evaluate the effect of 6-week supplementation with a daily dose of 2g of curcumin on VO2max and prooxidant/antioxidant homeostasis in middle-aged amateur long-distance runners during the preparatory period of the macrocycle. METHODS: Thirty runners were randomly assigned to a placebo group (PL) and a curcumin-supplemented group (CU). Their VO2max was assessed before supplementation and after 6 weeks of supplementation. Venous blood samples were collected from the participants at rest, immediately after exercise, and after 1h of recovery to evaluate the activity of antioxidant enzymes (SOD, CAT, GPx), non-enzymatic antioxidants (GSH, UA) and sirtuin 3 level (SIRT 3), as well as the levels of oxidative stress markers (TOS/TOC, MDA, and 8-OHdG) and muscle damage markers (CK, LDH, and Mb). RESULTS: VO2max, the activity of enzymatic antioxidants, the concentrations of non-enzymatic antioxidants, the levels of oxidative stress markers, and the levels of muscle damage markers did not change significantly in the CU group over 6 weeks of supplementation with curcumin. However, the resting concentration of SIRT 3 was found to be significantly higher (p ≤ 0.05) compared with pre-supplementation. CONCLUSION: Curcumin supplementation does not have a significant effect on VO2max and prooxidant/antioxidant homeostasis in runners.
- Klíčová slova
- Supplementation, antioxidant enzymes, flavonoids, men, non-enzymatic antioxidants, oxidative stress, prooxidant/antioxidant homeostasis, training,
- MeSH
- antioxidancia MeSH
- kurkumin * MeSH
- lidé MeSH
- potravní doplňky MeSH
- reaktivní formy kyslíku MeSH
- sirtuin 3 * MeSH
- superoxiddismutasa MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- randomizované kontrolované studie MeSH
- Názvy látek
- antioxidancia MeSH
- kurkumin * MeSH
- reaktivní formy kyslíku MeSH
- sirtuin 3 * MeSH
- superoxiddismutasa MeSH
Increasing evidence points to the respiratory Complex II (CII) as a source and modulator of reactive oxygen species (ROS). Both functional loss of CII as well as its pharmacological inhibition can lead to ROS generation in cells, with a relevant impact on the development of pathophysiological conditions, i.e. cancer and neurodegenerative diseases. While the basic framework of CII involvement in ROS production has been defined, the fine details still await clarification. It is important to resolve these aspects to fully understand the role of CII in pathology and to explore its therapeutic potential in cancer and other diseases.
- Klíčová slova
- OXPHOS, Respiratory complex II, cancer, mitochondria, reactive oxygen species, succinate, succinate dehydrogenase, tricarboxylic acid cycle,
- MeSH
- cílená molekulární terapie * MeSH
- lidé MeSH
- mitochondriální nemoci farmakoterapie metabolismus patologie MeSH
- mitochondrie metabolismus patologie MeSH
- reaktivní formy kyslíku metabolismus MeSH
- respirační komplex II metabolismus MeSH
- transport elektronů MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- reaktivní formy kyslíku MeSH
- respirační komplex II MeSH
OBJECTIVES: Rutin, quercetin-3-O-rutinoside, a natural flavonol glycoside, has shown various in vitro benefits with potential use treating human diseases, especially cardiovascular system disorders. Antioxidant properties are assumed to underlie the majority of these benefits. Yet rutin pro-oxidant properties have been reported as well. Our research group has recently shown aggravating effects on isoprenaline (ISO)-induced cardiotoxicity in Wistar:Han rats after 24 hours. METHODS: This study was designed to examine in more detail the reasons for the negative effects of rutin (11.5 and 46 mg/kg, i.v.) after administration of ISO (100 mg/kg, s.c.) in rats within 2 hours of continuous experiment and in the H9c2 cardiomyoblast-derived cell line. RESULTS: Like our previous findings, rutin did not (11.5 or 46 mg/kg, i.v.) reduce the ISO-induced mortality within 2 hours although the lower dose significantly reduced cardiac troponin T (cTnT) and partly improved the histological findings. In contrast, the higher dose increased the mortality in comparison with solvent (1.26% w/v sodium bicarbonate). This was not caused by any specific haemodynamic disturbances. It appears to be associated with oxidative stress as rutin enhanced intracellular reactive oxygen species formation in vitro and had the tendency to increase it in vivo. CONCLUSIONS: Rutin, likely due to its pro-oxidative effects, can exacerbate catecholamine cardiotoxicity depending on the dose used.
- Klíčová slova
- Catecholamine, Flavonoid, H9c2 cell line, Isoprenaline, Reactive oxygen species, Rutin, Wistar rat,
- MeSH
- buněčné linie MeSH
- dinoprost analogy a deriváty krev MeSH
- elektrokardiografie MeSH
- glutathion krev MeSH
- injekce intravenózní MeSH
- isoprenalin škodlivé účinky MeSH
- Kaplanův-Meierův odhad MeSH
- kardiotoxicita etiologie mortalita MeSH
- myokard patologie MeSH
- potkani Wistar MeSH
- reaktivní formy kyslíku metabolismus MeSH
- rutin aplikace a dávkování škodlivé účinky farmakokinetika MeSH
- srdce účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 8-epi-prostaglandin F2alpha MeSH Prohlížeč
- dinoprost MeSH
- glutathion MeSH
- isoprenalin MeSH
- reaktivní formy kyslíku MeSH
- rutin MeSH
OBJECTIVES: This study is an extension to our finding of direct anti-oxidant activities of lanthanide(III) complexes with the heterocyclic compound, 5-aminoorotic acid (AOA). In this experiment, we used AOA and coumarin-3-carboxylic acid as the two heterocyclic compounds with anti-oxidant potential, to produce the complexes with different lanthanides. METHODS: Lanthanide(III) complexes were tested on the iron-driven Fenton reaction. The product of this reaction, the hydroxyl radical, was detected by HPLC. RESULTS: All complexes as well as their ligands had positive or neutral effect on the Fenton reaction but their behavior was different. Both pure ligands in low concentration ratio to iron were inefficient in contrast to some of their complexes. Complexes of neodymium, samarium, gadolinium, and partly of cerium blocked the Fenton reaction at very low ratios (in relation to iron) but the effect disappeared at higher ratios. In contrast, lanthanum complexes appeared to be the most promising. Both blocked the Fenton reaction in a dose-dependent manner. CONCLUSION: Lanthanide(III) complexes were proven to block the iron-driven production of the hydroxyl radical. Second, the lanthanide(III) element appears to be crucial for the anti-oxidant effect. Overall, lanthanum complexes may be promising direct anti-oxidants for future testing.
- Klíčová slova
- 5-Aminoorotic acid, Anti-oxidant, Coumarin-3-carboxylic acid, Iron, Lanthanide(III),
- MeSH
- antioxidancia MeSH
- kumariny chemie MeSH
- kyselina orotová analogy a deriváty chemie MeSH
- lanthanoidy chemie MeSH
- ligandy MeSH
- peroxid vodíku chemie MeSH
- železo chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 5-aminoorotic acid MeSH Prohlížeč
- antioxidancia MeSH
- coumarin-3-carboxylic acid MeSH Prohlížeč
- Fenton's reagent MeSH Prohlížeč
- kumariny MeSH
- kyselina orotová MeSH
- lanthanoidy MeSH
- ligandy MeSH
- peroxid vodíku MeSH
- železo MeSH
OBJECTIVES: Salivary advanced glycation end-products (AGEs), advanced oxidation protein products (AOPP), total antioxidant capacity (TAC), and ferric reducing ability of saliva (FRAS) are increased in various diseases. Little data exist for these markers in the healthy population. The aim of this study was to assess the inter-individual and intra-individual variability of AGEs, AOPP, TAC, and FRAS in the saliva of young healthy individuals. METHODS: Unstimulated saliva samples were collected from 16 females and 18 males daily over a period of 30 days. Markers were measured using spectrophotometric and spectrofluorometric microplate-based methods. RESULTS: All salivary markers measured were significantly higher in men than in women (P < 0.05 for AGEs; P < 0.001 for AOPP, TAC, and FRAS). The inter-individual variability was approximately 60% for AGEs and AOPP and 30-40% for TAC and FRAS in both genders. The inter-individual variability of FRAS was higher in men vs. women (P < 0.01). Intra-individual variability ranged from 20% for TAC, to 30% for AGES and FRAS and 45% for AOPP. DISCUSSION: Intra-individual variability of salivary AGEs, AOPP, TAC, and FRAS indicates that their use is currently limited to large cohort studies. Identifying the underlying factors related to the high inter-individual and intra-individual variability is needed. Sex differences should be considered in future studies.
- Klíčová slova
- Biomarkers of oxidative stress, Carbonyl stress, Sex difference,
- Publikační typ
- časopisecké články MeSH
Quinolinic acid increased the generation of lipid peroxidation products by isolated rat brain microvessels in vitro. The effect was inhibited both by a specific NMDA receptor antagonist D-2-amino-5-phosphonovaleric acid and by reduced glutathione (GSH). Furthermore, quinolinic acid displaced specific binding of [(3)H]-L-glutamate by cerebral microvessel membranes, particularly in the presence of NMDA receptor co-agonist (glycine) and modulator (spermidine). We conclude that quinolinic acid can cause potentially cytotoxic lipid peroxidation in brain microvessels via an NMDA receptor mediated mechanism.
- MeSH
- 2-amino-5-fosfonovalerát farmakologie MeSH
- antagonisté excitačních aminokyselin farmakologie MeSH
- glutathion farmakologie MeSH
- krysa rodu Rattus MeSH
- mikrocirkulace účinky léků fyziologie MeSH
- mozkový krevní oběh účinky léků fyziologie MeSH
- peroxidace lipidů fyziologie MeSH
- potkani Wistar MeSH
- receptory N-methyl-D-aspartátu fyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 2-amino-5-fosfonovalerát MeSH
- antagonisté excitačních aminokyselin MeSH
- glutathion MeSH
- receptory N-methyl-D-aspartátu MeSH
