OBJECTIVE: The aim of this study was to evaluate changes in the relative counts of different leukocyte subsets in peripheral and umbilical cord blood in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) with respect to the presence of intraamniotic inflammation (IAI) and fetal inflammatory response syndrome (FIRS). METHODS: Fifty-two women with singleton pregnancies complicated by PPROM were included in this study. From samples of peripheral and umbilical cord blood, relative counts of these leukocyte subpopulations were determined using multicolor flow cytometry: granulocytes, monocytes, lymphocytes, T cells and their subpopulations, B cells and their subpopulations, and NK cells and their subpopulations. IAI was defined as increased concentrations of interleukin 6 in the amniotic fluid. Amniotic fluid samples were obtained by transabdominal amniocentesis. RESULTS: Women with IAI had higher relative counts of monocytes (p = 0.04) in peripheral blood. There was an increased relative number of granulocytes (p = 0.003) and a decreased number of lymphocytes (p = 0.0048), helper CD4+ T cells (p = 0.019), NK cells (p = 0.0001) within leukocytes, NK cells within lymphocytes (p = 0.003) and CD16+ NK cells within NK cells (p = 0.005) in umbilical cord blood samples of women with FIRS. However, after adjusting the results for gestational age at sampling, all differences disappeared. CONCLUSIONS: The presence of IAI or FIRS is not accompanied by significant changes in the relative counts of immune cells in peripheral blood or umbilical cord blood in pregnancies complicated by PPROM.

• Klíčová slova
• FIRS, PPROM, flow cytometry, intraamniotic inflammation, leukocyte subpopulations,
• MeSH
• chorioamnionitida imunologie   krev   MeSH
• dospělí MeSH
• fetální krev * imunologie   cytologie   MeSH
• interleukin-6 krev   metabolismus   MeSH
• leukocyty imunologie   MeSH
• lidé MeSH
• plodová voda imunologie   metabolismus   MeSH
• počet leukocytů MeSH
• předčasný odtok plodové vody * imunologie   krev   MeSH
• průtoková cytometrie MeSH
• syndrom systémové zánětlivé reakce imunologie   krev   MeSH
• těhotenství MeSH
• zánět imunologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• interleukin-6 MeSH

In this study, pentraxin 3 (PTX3), C-reactive protein (CRP), and serum amyloid P component (SAP) concentrations in the amniotic fluid of women with preterm pre-labor rupture of membranes (PPROM) were evaluated based on evidence of microbial invasion of the amniotic cavity (MIAC), intra-amniotic inflammation (IAI), and microbial-associated IAI. A total of 149 women with PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. Amniotic fluid PTX3, SAP, and CRP concentrations were assessed using enzyme-linked immunosorbent assay. PTX3 and CRP concentrations were higher in women with MIAC, IAI, and microbial-associated IAI than in women without these conditions. SAP concentrations were only higher in the presence of IAI and microbial-associated IAI. Amniotic fluid PTX3 concentrations of 11 ng/mL were found to be the best value for identifying the presence of microbial-associated IAI and IAI in women with PPROM. To conclude, amniotic fluid pentraxins are involved in intra-amniotic inflammatory responses in pregnancies complicated by PPROM.

• Klíčová slova
• innate immunity, intra-amniotic inflammation, microbial invasion of the amniotic fluid, pattern-recognition molecules, preterm delivery,
• MeSH
• amniocentéza metody   MeSH
• biologické markery metabolismus   MeSH
• C-reaktivní protein metabolismus   MeSH
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• plodová voda metabolismus   MeSH
• předčasná porodní činnost metabolismus   MeSH
• předčasný odtok plodové vody metabolismus   MeSH
• retrospektivní studie MeSH
• sérový amyloidový protein metabolismus   MeSH
• těhotenství MeSH
• zánět metabolismus   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické markery MeSH
• C-reaktivní protein MeSH
• PTX3 protein MeSH Prohlížeč
• sérový amyloidový protein MeSH

PROBLEM: Investigating the stress response in the central cotyledon zone of placental tissue in pregnancies with PPROM, PTB, and at term in labor. METHOD OF STUDY: Gene expression of Hsp27, Hsp60, Hsp70, Hsp90, and HspBP1 was compared between these particular groups. Correlation between variables including Hsp gene expression in placental tissue and the gestational age at delivery, WBC count at admission, and serum levels of CRP at admission in patients with PPROM and PTB was determined. RESULTS: Both PPROM and PTB pregnancies were associated with altered Hsp gene expression profile. While PPROM and PTB always induced upregulation of Hsp27 and Hsp60, downregulation of Hsp70 and HspBP1 was present entirely in patients with PPROM. HspBP1 expression profile was also able to differentiate between PPROM and PTB pregnancies. The highest mRNA levels of Hsp60 and Hsp70 were detected in PTB pregnancies with elevated CRP levels at admission. Some of the examined Hsp displayed increased expression with advancing gestational age in both groups (PPROM: Hsp27, Hsp70, and Hsp90; and PTB: Hsp27). CONCLUSION: Upregulation of Hsp27 is a common phenomenon shared between pregnancies affected with PTB and PPROM. On the other hand, downregulation of Hsp70 and HspBP1 represents a unique feature of PPROM.

• Klíčová slova
• expression profile, heat shock proteins, placental tissue, preterm delivery, real-time PCR,
• MeSH
• adaptorové proteiny signální transdukční genetika   metabolismus   MeSH
• amnion patologie   MeSH
• chaperon hsp60 genetika   metabolismus   MeSH
• dospělí MeSH
• lidé MeSH
• mitochondriální proteiny genetika   metabolismus   MeSH
• molekulární chaperony MeSH
• placenta fyziologie   MeSH
• předčasná porodní činnost genetika   imunologie   MeSH
• proteiny tepelného šoku HSP27 genetika   metabolismus   MeSH
• proteiny tepelného šoku HSP70 genetika   metabolismus   MeSH
• proteiny tepelného šoku HSP90 genetika   metabolismus   MeSH
• proteiny tepelného šoku MeSH
• regulace genové exprese MeSH
• samovolný potrat genetika   imunologie   MeSH
• těhotenství MeSH
• transkriptom MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• adaptorové proteiny signální transdukční MeSH
• chaperon hsp60 MeSH
• HSPB1 protein, human MeSH Prohlížeč
• HSPBP1 protein, human MeSH Prohlížeč
• HSPD1 protein, human MeSH Prohlížeč
• mitochondriální proteiny MeSH
• molekulární chaperony MeSH
• proteiny tepelného šoku HSP27 MeSH
• proteiny tepelného šoku HSP70 MeSH
• proteiny tepelného šoku HSP90 MeSH
• proteiny tepelného šoku MeSH

Although sera and all external secretions contain antibodies to human immunodeficiency virus (HIV), their levels, specificity, isotypes, and relevant effector functions display a great degree of variability. Antibodies that bind HIV antigens and neutralize the virus are predominantly associated with the IgG isotype in sera and in all external secretions, even where total levels of IgG are much lower than those of IgA. Rectal fluid that contains high IgA, but low IgG levels, displayed low neutralizing activity independent of antibodies. Therefore, external secretions should be evaluated before and after selective depletion of Ig. At the systemic level, HIV-specific IgA may interfere with the effector functions of IgG, as suggested by recent studies of individuals systemically immunized with an experimental HIV vaccine. Although HIV-specific IgG and IgA antibodies may exhibit their protective activities at mucosal surfaces through interference with viral entry and local neutralization at the systemic level, such antibodies may display discordant effector functions.

• Klíčová slova
• Antibody responses, human immunodeficiency virus, mucosal immunity, secretory IgA and IgG,
• MeSH
• HIV antigeny imunologie   MeSH
• HIV infekce imunologie   MeSH
• HIV protilátky krev   imunologie   MeSH
• HIV-1 imunologie   MeSH
• humorální imunita MeSH
• imunoglobulin A krev   imunologie   MeSH
• imunoglobulin G krev   imunologie   MeSH
• lidé MeSH
• neutralizující protilátky krev   imunologie   MeSH
• sliznice imunologie   metabolismus   virologie   MeSH
• vaginální výplach MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• HIV antigeny MeSH
• HIV protilátky MeSH
• imunoglobulin A MeSH
• imunoglobulin G MeSH
• neutralizující protilátky MeSH

PROBLEM: This study compares the frequencies of plasminogen activator inhibitor-1 (-675) 4G/5G polymorphism and its relationship with eight antiphospholipid antibodies (aPLs) in serum of 157 patients with repeated pregnancy loss (RPL). METHOD OF STUDY: PAI-1 (-675) 4G/5G polymorphism was determined using standard PCR-RFLP method. Enzyme-linked immunosorbent assay was used for the detection of aPLs against ph-serine, ph-ethanolamine, ph-inositol, ph-DL-glycerol, phosphatidic acid, annexin V, cardiolipin, and beta2-GPI. Allelic frequency and distribution of genotypes were calculated. The prevalence of the risk conferring 4G allele and 4G/4G homozygous genotype in patients and controls was compared, and the correlation between aPLs positivity and PAI-1 4G/4G genotype was tested by chi-square test. RESULTS: Statistically highly significant correlation between RPL and PAI-1 (-675) 4G/4G genotype was found. No correlation between PAI-1 (-675) 4G/5G polymorphism and the presence of antiphospholipid antibodies in RPL patients was observed. CONCLUSIONS: PAI-1 (-675) 4G/4G homozygous genotype increases the risk of RPL independently from the aPLs positivity.

• MeSH
• antifosfolipidové protilátky krev   MeSH
• běloši genetika   MeSH
• dospělí MeSH
• frekvence genu MeSH
• genotyp MeSH
• habituální potrat krev   genetika   imunologie   MeSH
• inhibitor aktivátoru plazminogenu 1 genetika   MeSH
• lidé MeSH
• polymorfismus genetický MeSH
• těhotenství MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• antifosfolipidové protilátky MeSH
• inhibitor aktivátoru plazminogenu 1 MeSH

PROBLEM: Up to 20% of pregnancies end in the first trimester by spontaneous abortion, but a significant number remains unexplained. The aim of this study is to investigate the role of variants within the gene for apolipoprotein E (APOE) in the genetic determination of spontaneous abortions. METHOD OF STUDY: We collected DNA from 410 tissue samples of spontaneous abortions, and APOE was genotyped by PCR-RFLP method. The frequencies were compared with a population sample of adults (N = 2606) and with a positive control (1060 women with at least two children). RESULTS: The frequencies of the APOE genotypes in abortions (APOE2E2 + E3E2 = 0.132; APOE3E3 = 0.661; APOE3E4 + E4E4 = 0.195; APOE4E2 = 0.012) did not significantly differ (P = 0.604) from the frequencies in analyzed adult population study (APOE2E2 + E3E2 = 0.132; APOE3E3 = 0.686; APOE3E4 + E4E4 = 0.169; APOE4E2 = 0.014) or from the positive control (APOE2E2 + E3E2 = 0.133; APOE3E3 = 0.691; APOE3E4 + E4E4 =0.166; APOE4E2 = 0.010; P = 0.592). CONCLUSION: Our study suggests that APOE may not be associated with spontaneous abortions in Caucasians.

• MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• polymorfismus genetický * MeSH
• samovolný potrat genetika   MeSH
• těhotenství MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

PROBLEM: The aim of present study was to investigate the effects of antioxidant lycopene on soluble receptor for advanced glycation end products (sRAGE) levels in blood and seminal plasma in normospermic males. METHODS: Study included 15 fertile volunteers and 13 normospermic male partners from infertile relationships. The treatment was 12-week administration of 20 mg of lycopene or placebo followed by crossover and treatment for a further 12 weeks. The ELISA kit Quantikine(®) was used to determine sRAGE levels. RESULTS: Lycopene administration decreased sRAGE levels in seminal plasma in fertile volunteers (controls) as well as in male partners in the infertile relationships group (P=0.008 and P=0.012, respectively). No significant effect of lycopene on sRAGE in blood plasma was found in either group, but seminal plasma sRAGE was significantly suppressed. CONCLUSION: Lycopene decreased sRAGE in seminal, but not in blood plasma. This may be because of selective local uptake of lycopene in the male reproductive tract, namely in prostate. Decreased sRAGE may be caused by lycopene suppression of oxidative stressors and explain in part the putative improvement in fertility reported after lycopene treatment.

• MeSH
• biologické markery analýza   MeSH
• karotenoidy farmakologie   MeSH
• lidé MeSH
• lykopen MeSH
• prostata chemie   účinky léků   MeSH
• receptor pro konečné produkty pokročilé glykace MeSH
• receptory imunologické analýza   MeSH
• sperma chemie   účinky léků   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• biologické markery MeSH
• karotenoidy MeSH
• lykopen MeSH
• receptor pro konečné produkty pokročilé glykace MeSH
• receptory imunologické MeSH

PROBLEM: To analyze the relation of the fertility and pregnancy of women of childbearing age to the intracellular (IC) production of tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), and interleukins 2 and 4 (IL-2 and IL-4). METHOD OF STUDY: Intracellular cytokine production in peripheral blood CD3(+) CD4(+) lymphocytes was analyzed by flow cytometry in 185 women being treated for infertility and 50 fertile women of childbearing age. RESULTS: Infertile women have a significantly higher IC production of TNF-α, IFN-γ, IL-2, and IL-4 and higher ratios of TNF-α/IL-2, TNF-α/IL-4, and TNF-α/IFN-γ compared to the fertile women. CONCLUSION: Cytokines produced by Th lymphocytes are important in orchestrating the immune response during conception, and Th-cell dysregulation could be a reason for infertility.

• MeSH
• cytokiny biosyntéza   krev   MeSH
• dospělí MeSH
• fertilita imunologie   MeSH
• interferon gama biosyntéza   krev   MeSH
• interleukin-2 biosyntéza   krev   MeSH
• interleukin-4 biosyntéza   krev   MeSH
• lidé MeSH
• průtoková cytometrie MeSH
• T-lymfocyty pomocné-indukující imunologie   metabolismus   MeSH
• těhotenství imunologie   MeSH
• TNF-alfa biosyntéza   krev   MeSH
• ženská infertilita imunologie   patofyziologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• těhotenství imunologie   MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• cytokiny MeSH
• interferon gama MeSH
• interleukin-2 MeSH
• interleukin-4 MeSH
• TNF-alfa MeSH

PROBLEM: The present work was undertaken to investigate the occurence of autoantibodies to eight various phospholipids in time of urgent termination of the pregnancy (sectio caesarea) in patients in reproductive age with severe preeclamptic symptoms. METHOD OF STUDY: Autoantibodies against annexin V, ph-serine, ph-ethanolamine, ph-inositol, ph-DL-glycerol, cardiolipin, beta2-glycoprotein I (beta2-GPI), and phosphatidic acid were studied by ELISA methods. RESULTS: Increased levels of IgA-beta2-glycoprotein I, IgG-beta2-glycoprotein I, IgG- anti-ph-serine, and IgG-anticardiolipin were found in sera of preeclamptic women in the time of urgent sectio caesarea when compared to the control group with physiological pregnancy. CONCLUSION: Supposed increase in various antiphospholipid antibodies (aPLs) levels due to the stress during the short time of admission and a need for a quick medical decision to terminate the pregnancy was not unambiguously proven, but our results are evidently influenced by the current urgent life-saving treatment.

• MeSH
• antifosfolipidové protilátky krev   MeSH
• beta-2-glykoprotein I imunologie   MeSH
• dospělí MeSH
• ELISA MeSH
• fosfoserin imunologie   MeSH
• lidé MeSH
• placentární oběh imunologie   MeSH
• potrat léčebný MeSH
• preeklampsie krev   imunologie   patofyziologie   terapie   MeSH
• těhotenství MeSH
• trombóza MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antifosfolipidové protilátky MeSH
• beta-2-glykoprotein I MeSH
• fosfoserin MeSH

PROBLEM: The aim of study was to investigate identical female twins born in 1977 suffered from autoimmune diseases (twin A - Sjogren's syndrome, and twin B - systemic lupus erythematosus). METHOD OF STUDY: It was refer retrospective analysis of both sisters suffered beside autoimmune alterations (Sjogren's syndrome and systemic lupus erythematosus) also from gynecological diseases (twin A - praecancerosis of cervix uteri, twin B - carcinoma vaginae). RESULTS: Relationships between disease activities and severities in the female twins were similar and the treatments were directed according to clinical symptoms and laboratory results. Dramatic change, unfortunately, occurred with twin B. The reason may be the association between SLE activity (lupus nephritis), hematological complication (leukopenia) and oncological vaginal recidivation. CONCLUSION: Association between autoimmune disease and gynecological cancer (or praecancerosis) is major risk than without immunology deviation. Twin A is periodically gynecologically observed.

• MeSH
• dospělí MeSH
• dvojčata monozygotní * MeSH
• fatální výsledek MeSH
• lidé MeSH
• nádory děložního čípku komplikace   diagnóza   patologie   patofyziologie   MeSH
• nádory vaginy komplikace   diagnóza   patologie   patofyziologie   MeSH
• nemoci u dvojčat * MeSH
• prekancerózy komplikace   diagnóza   patologie   patofyziologie   MeSH
• retrospektivní studie MeSH
• Sjögrenův syndrom komplikace   diagnóza   patologie   patofyziologie   MeSH
• spinocelulární karcinom komplikace   diagnóza   patologie   patofyziologie   MeSH
• systémový lupus erythematodes komplikace   diagnóza   patologie   patofyziologie   MeSH
• těhotenství MeSH
• vedení porodu MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH