Application of the concept of corporate social responsibility (CSR) is of fundamental importance for the future existence and success of gambling operators, the activity of which is linked to a risk of addiction to gambling together with the negative impacts of this on the health and wellbeing of gamblers, families and society as a whole. Gambling operators must apply and effectively communicate socially responsible activities in the economic, environmental, ethical, social and philanthropic fields and in particular in the field of responsible gambling. Nowadays, it is necessary to ensure communication via modern channels in the form of corporate websites and social networks. This study evaluates the level of web-based CSR communication by gambling operators which operate in one of the post-communist countries-the Czech Republic (114 companies). It at the same time provides an alternative method of evaluating the level of CSR communication by gambling operators which makes it possible to uncover weaknesses and room for improvement on the part of the gambling operator's CSR communication practices. The results of the study show that the level of web-based CSR communication of gambling operators operating in the Czech Republic is low, both in comparison with controversial companies and also with non-controversial companies. Economic responsibility activities, responsible gambling and philanthropic responsibility activities are communicated to the greatest extent. On the contrary, ethical responsibility activities are communicated the least.

• Klíčová slova
• Corporate social responsibility, Czech Republic, Gambling operators, Responsible gambling, Web-based communication,
• MeSH
• dospělí MeSH
• hráčství epidemiologie   MeSH
• internet * MeSH
• lidé MeSH
• návykové chování epidemiologie   MeSH
• organizační kultura MeSH
• sociální chování MeSH
• sociální odpovědnost * MeSH
• veřejná politika MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

BACKGROUND: Users with experience of eating disorders use the internet as a source of information, whether for prorecovery activities (such as web-based treatment, looking for information, support, and sharing) or activities that promote eating disorder behavior as a desirable lifestyle choice (such as pro-eating disorder communities and reading and creating pro-eating disorder posts). Their assessment of web-based eating disorder-related information is crucial for understanding the context of the illness and for health professionals and their web-based interventions. OBJECTIVE: This study aimed to understand the criteria young women with the experience of eating disorders use in evaluating eating disorder-related web-based information and what eating disorder-related characteristics of these women are involved in their evaluation. METHODS: We analyzed 30 semistructured individual interviews with Czech women aged 16 to 28 years with past or present eating disorder experience using a qualitative approach. Thematic analysis was adopted as an analytical tool. RESULTS: The specifics of eating disorder phases (the disorder stage and the treatment process) emerged as important aspects in the process of information assessment. Other specific characteristics of respondents (eg, motivation, abilities, and resources) addressed how the respondents arrived at certain web-based information and how they evaluated it. In addition, the respondents described some content cues as features of information (eg, novelty and social information pooling). Another finding is that other users' attitudes, experiences, activities, and personal features are involved in the information evaluation of these users and the information presented by them. Finally, the respondents evaluated the websites' visual look and graphic components. CONCLUSIONS: This study shows that web-based information evaluation reported by women with experience of eating disorders is a complex process. The assessment is influenced by current personal characteristics related to the illness (mainly the motivation for maintaining or curing the eating disorder) using cues associated with information content, other users, and website look. The study findings have important implications for health professionals, who should ask their clients questions about web-based communities and their needs to understand what information and sources they choose.

• Klíčová slova
• Czech women, eating disorders, web-based health information,
• MeSH
• internet MeSH
• lidé MeSH
• motivace MeSH
• poruchy příjmu potravy * terapie   MeSH
• zdravotnický personál MeSH
• životní styl MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

AIM: To describe a randomized controlled trial protocol that will evaluate the effectiveness of two web-based genomic nursing education interventions. BACKGROUND: Preparing future nurses to be competent in genetic and genomic concepts is fundamental to ensure appropriate clinical application. However, genetics-genomics concepts are still new in the field of nursing. Little is known about what type and kind of web-based nursing education is effective in improving the knowledge of nursing students. To address these knowledge gaps, a web-based 'Genomic Nursing Education Intervention' will be developed and compared with an existing online education programme. DESIGN: A randomized controlled trial of two groups with pre-test and repeated posttesting. METHODS: The Genomic Nursing Concept Inventory, a validated tool, will be used to assess the genetics-genomics knowledge of nursing students. Participants will be randomly allocated to either a control or an intervention group. The control group will receive the standard web-based nursing education, while the intervention group will receive a newly developed web-based education intervention. Outcome measures include the students' knowledge level of nursing genetics-genomics concepts. Participants will be retested at 3 and 6 months. CONCLUSION: Current evidence shows that ensuring nurses have adequate education in genetic-genomic concepts is challenging. This study will demonstrate which of two web-based nursing education methods is more effective in teaching genetic-genomic concepts. This research project will better prepare the nursing profession in their careers for the emerging advance technologies in genetics-genomics and personalized health care. IMPACT: Current evidence shows major challenges in ensuring that nurses have adequate education in genetics-genomics concepts. Less is known about what approaches to web-based education are effective to improve the knowledge gaps of nursing students in genetics-genomics concepts. This study will determine which type of web-based nursing education is effective in improving the genetics-genomics knowledge of nursing students. This research project will help better prepare nurses in dealing with advances in genetics-genomics in their careers. TRIAL REGISTRATION: This study is registered in ClinicalTrials.gov (ID number NCT03963687) https://clinicaltrials.gov/show/NCT03963687.

目标: 描述一个评估两种基于网络基因组护理教育干预措施效果的随机对照试验方案。 背景资料: 培养未来的护士在基因和基因组概念方面的能力是确保其掌握适当临床应用的基础。然而,遗传学-基因组学的概念在护理领域仍然算是新知识。目前还不清楚怎样的网络护理教育能有效地提高护理生的知识水平。为解决这些知识缺口,我们将开发网络“基因组护理教育干预”,并与现有的在线教育计划进行比较。 设计: 对测试前组和重复测试后组进行随机对照试验。 方法: 基因组护理概念量表是一个经过验证的工具,将用于评估护理生的遗传学-基因组学知识。参与者将被随机分配到对照组或干预组。对照组接受标准的网络护理教育,干预组接受新开发的网络教育干预。结果测量包含了学生对护理遗传学-基因组学概念的知识水平。在3个月和6个月时对参与者进行重新测试。 结论: 目前的证据表明,确保护士掌握基因-基因组概念方面充足的教育具有挑战性。这项研究证明两种基于网络的护理教育方法中哪一种能更有效地教授基因-基因组概念。这项研究项目将更好地为护理专业人士的职业生涯做好准备,以适应遗传学领域新兴的先进技术--即基因组学和个性化医疗保健。 影响: 目前的证据显示,确保护士掌握充足的遗传学-基因组学概念教育存在重大的挑战性。目前还不清楚哪一种网络教育方法可以有效地改善护士生在遗传学-基因组学概念方面的知识差距。本研究将确定哪类网络护理教育能有效提高护士生在遗传学-基因组学方面的知识。这项研究项目将有助于护士在职业生涯中更好地应对在遗传学-基因组学方面取得的进步。 试验注册: 本研究在ClinicalTrials.gov上注册(注册编号:NCT03963687)https://clinicaltrials.gov/show/NCT03963687.

• Klíčová slova
• education, genetics, genomics, midwives, nurses, nursing, protocol, web based,
• MeSH
• genomika výchova   MeSH
• internet MeSH
• lidé MeSH
• randomizované kontrolované studie jako téma MeSH
• studenti ošetřovatelství * MeSH
• studium ošetřovatelství bakalářské * MeSH
• studium ošetřovatelství * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• protokol klinické studie MeSH

BioDat is a software tool that simplifies routine recording, storing and analyzing technical and biological 1D signals. The basic features of the BioDat software are: database format based on PhysioNet, a shared database of signals running in network environment accessible for users with permissions, importing and/or exporting data from and/or to different formats (d-file, EDF, ASCII, Matlab), accessing the database through HTTP(S) and ordinary web browsers. The application is written in Java and runs both standalone and as installation-free Java applet.

• MeSH
• databáze faktografické * MeSH
• internet * MeSH
• počítačová grafika MeSH
• počítačové zpracování signálu * MeSH
• software * MeSH
• systémy řízení databází * MeSH
• ukládání a vyhledávání informací metody   MeSH
• uživatelské rozhraní počítače * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Enzymes are in high demand for very diverse biotechnological applications. However, natural biocatalysts often need to be engineered for fine-tuning their properties towards the end applications, such as the activity, selectivity, stability to temperature or co-solvents, and solubility. Computational methods are increasingly used in this task, providing predictions that narrow down the space of possible mutations significantly and can enormously reduce the experimental burden. Many computational tools are available as web-based platforms, making them accessible to non-expert users. These platforms are typically user-friendly, contain walk-throughs, and do not require deep expertise and installations. Here we describe some of the most recent outstanding web-tools for enzyme engineering and formulate future perspectives in this field.

• MeSH
• biotechnologie * MeSH
• internet * MeSH
• rozpustnost MeSH
• výpočetní biologie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

Protein structure determines biological function. Accurately conceptualizing 3D protein/ligand structures is thus vital to scientific research and education. Virtual reality (VR) enables protein visualization in stereoscopic 3D, but many VR molecular-visualization programs are expensive and challenging to use; work only on specific VR headsets; rely on complicated model-preparation software; and/or require the user to install separate programs or plugins. Here we introduce ProteinVR, a web-based application that works on various VR setups and operating systems. ProteinVR displays molecular structures within 3D environments that give useful biological context and allow users to situate themselves in 3D space. Our web-based implementation is ideal for hypothesis generation and education in research and large-classroom settings. We release ProteinVR under the open-source BSD-3-Clause license. A copy of the program is available free of charge from http://durrantlab.com/protein-vr/, and a working version can be accessed at http://durrantlab.com/pvr/.

• MeSH
• internet * MeSH
• konformace proteinů MeSH
• proteiny * chemie   ultrastruktura   MeSH
• virtuální realita * MeSH
• výpočetní biologie metody   MeSH
• zobrazování trojrozměrné metody   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• proteiny * MeSH

PURPOSE: Rapid identification and characterization of multidrug-resistant Klebsiella pneumoniae strains is necessary due to the increasing frequency of severe infections in patients. The decreasing cost of next-generation sequencing enables us to obtain a comprehensive overview of genetic information in one step. The aim of this study is to demonstrate and evaluate the utility and scope of the application of web-based databases to next-generation sequenced (NGS) data. METHODOLOGY: The whole genomes of 11 clinical Klebsiella pneumoniae isolates were sequenced using Illumina MiSeq. Selected web-based tools were used to identify a variety of genetic characteristics, such as acquired antimicrobial resistance genes, multilocus sequence types, plasmid replicons, and identify virulence factors, such as virulence genes, cps clusters, urease-nickel clusters and efflux systems. RESULTS: Using web-based tools hosted by the Center for Genomic Epidemiology, we detected resistance to 8 main antimicrobial groups with at least 11 acquired resistance genes. The isolates were divided into eight sequence types (ST11, 23, 37, 323, 433, 495 and 562, and a new one, ST1646). All of the isolates carried replicons of large plasmids. Capsular types, virulence factors and genes coding AcrAB and OqxAB efflux pumps were detected using BIGSdb-Kp, whereas the selected virulence genes, identified in almost all of the isolates, were detected using CLC Genomic Workbench software. CONCLUSION: Applying appropriate web-based online tools to NGS data enables the rapid extraction of comprehensive information that can be used for more efficient diagnosis and treatment of patients, while data processing is free of charge, easy and time-efficient.

• Klíčová slova
• BIGSdb-Kp, Klebsiella pneumoniae, MLST, PlasmidFinder, ResFinder, next generation sequencing,
• MeSH
• antibakteriální látky farmakologie   MeSH
• databáze genetické MeSH
• DNA bakterií genetika   MeSH
• genom bakteriální * MeSH
• internet MeSH
• Klebsiella pneumoniae genetika   MeSH
• mnohočetná bakteriální léková rezistence genetika   MeSH
• multilokusová sekvenční typizace MeSH
• plazmidy MeSH
• sekvence nukleotidů MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• DNA bakterií MeSH

Automatic system for ranking of educational health care related web resources based on quality criteria was developed. Automatic retrieval of new web resources from well-known directories followed by their automatic evaluation is a keystone of the system. The system provides this information: a) location of the resource and its author, b) number of back links to the resource, c) checks presence of electronic labels of quality. Currently the system encompasses more than 500 links to educational web resources divided into 47 medical specialities. The resources in all categories are ranked according to their link popularity, the electronic labels of quality are presented. History of link popularity is recorded and might be displayed at every resource. There are 2 language editions. The system operates automatically, the editors check and correct the retrieved values. The described system adds to webcite indexing criteria for objective evaluation of quality of webpages. It is useful for the selection of optimum education resources in health care.

• Publikační typ
• časopisecké články MeSH

BACKGROUND: Structural biomolecular data are commonly stored in the PDB format. The PDB format is widely supported by software vendors because of its simplicity and readability. However, the PDB format cannot fully address many informatics challenges related to the growing amount of structural data. To overcome the limitations of the PDB format, a new textual format mmCIF was released in June 1997 in its version 1.0. mmCIF provides extra information which has the advantage of being in a computer readable form. However, this advantage becomes a disadvantage if a human must read and understand the stored data. While software tools exist to help to prepare mmCIF files, the number of available systems simplifying the comprehension and interpretation of the mmCIF files is limited. FINDINGS: In this paper we present mmView - a cross-platform web-based application that allows to explore comfortably the structural data of biomacromolecules stored in the mmCIF format. The mmCIF categories can be easily browsed in a tree-like structure, and the corresponding data are presented in a well arranged tabular form. The application also allows to display and investigate biomolecular structures via an integrated Java application Jmol. CONCLUSIONS: The mmView software system is primarily intended for educational purposes, but it can also serve as a useful research tool. The mmView application is offered in two flavors: as an open-source stand-alone application (available from http://sourceforge.net/projects/mmview) that can be installed on the user's computer, and as a publicly available web server.

• Publikační typ
• časopisecké články MeSH

Biomolecular channels play important roles in many biological systems, e.g. enzymes, ribosomes and ion channels. This article introduces a web-based interactive MOLEonline 2.0 application for the analysis of access/egress paths to interior molecular voids. MOLEonline 2.0 enables platform-independent, easy-to-use and interactive analyses of (bio)macromolecular channels, tunnels and pores. Results are presented in a clear manner, making their interpretation easy. For each channel, MOLEonline displays a 3D graphical representation of the channel, its profile accompanied by a list of lining residues and also its basic physicochemical properties. The users can tune advanced parameters when performing a channel search to direct the search according to their needs. The MOLEonline 2.0 application is freely available via the Internet at http://ncbr.muni.cz/mole or http://mole.upol.cz.

• MeSH
• cytochrom P-450 CYP3A chemie   MeSH
• enzymy chemie   MeSH
• internet MeSH
• iontové kanály chemie   MeSH
• konformace proteinů MeSH
• molekulární modely MeSH
• počítačová grafika MeSH
• ribozomy chemie   MeSH
• software * MeSH
• uživatelské rozhraní počítače MeSH
• velké podjednotky ribozomu archebakteriální chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• cytochrom P-450 CYP3A MeSH
• enzymy MeSH
• iontové kanály MeSH