Metastatic MHC class I-negative mouse cells derived by transformation with human papillomavirus type 16
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11225590
PubMed Central
PMC2363731
DOI
10.1054/bjoc.2000.1615
PII: S000709200091615X
Knihovny.cz E-resources
- MeSH
- Cell Line MeSH
- Neoplasms, Experimental immunology metabolism pathology MeSH
- Gene Expression MeSH
- Histocompatibility Antigens Class I metabolism MeSH
- Histocompatibility Antigens Class II metabolism MeSH
- Immunoblotting MeSH
- Immunohistochemistry MeSH
- Keratins analysis MeSH
- Humans MeSH
- Neoplasm Metastasis pathology MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Tumor Cells, Cultured cytology radiation effects MeSH
- Oncogene Proteins, Viral genetics MeSH
- Papillomaviridae genetics MeSH
- Papillomavirus E7 Proteins MeSH
- Plasmids administration & dosage genetics immunology MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Flow Cytometry MeSH
- ras Proteins genetics metabolism MeSH
- DNA, Recombinant MeSH
- Repressor Proteins * MeSH
- RNA genetics metabolism MeSH
- Transfection MeSH
- Cell Line, Transformed MeSH
- Neoplasm Transplantation MeSH
- Cell Transformation, Viral * MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- E6 protein, Human papillomavirus type 16 MeSH Browser
- Histocompatibility Antigens Class I MeSH
- Histocompatibility Antigens Class II MeSH
- Keratins MeSH
- oncogene protein E7, Human papillomavirus type 16 MeSH Browser
- Oncogene Proteins, Viral MeSH
- Papillomavirus E7 Proteins MeSH
- ras Proteins MeSH
- DNA, Recombinant MeSH
- Repressor Proteins * MeSH
- RNA MeSH
In the endeavour to develop a model for studying gene therapy of cancers associated with human papillomaviruses (HPVs), mouse cells were transformed with the HPV type 16 (HPV16) and activated H-ras oncogenes. This was done by cotransfection of plasmid p16HHMo, carrying the HPV16 E6/E7 oncogenes, and plasmid pEJ6.6, carrying the gene coding for human H-ras oncoprotein activated by the G12V mutation, into secondary C57BL/6 mouse kidney cells. An oncogenic cell line, designated MK16/1/IIIABC, was derived. The epithelial origin of the cells was confirmed by their expression of cytokeratins. No MHC class I and class II molecules were detected on the surface of MK16/1/IIIABC cells. Spontaneous metastases were observed in lymphatic nodes and lungs after prolonged growth of MK16/1/IIIABC-induced subcutaneous tumours. Lethally irradiated MK16/1/IIIABC cells induced protection against challenge with 10(5) homologous cells, but not against a higher cell dose (5 x 10(5)). Plasmids p16HHMo and pEJ6.6 were also used for preventive immunization of mice. In comparison with a control group injected with pBR322, they exhibited moderate protection, in terms of prolonged survival, against MK16/1/IIIABC challenge (P < 0.03). These data suggest that MK16/1/IIIABC cells may serve as a model for studying immune reactions against HPV16-associated human tumours.
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