MRI of transplanted pancreatic islets
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15562474
DOI
10.1002/mrm.20282
Knihovny.cz E-resources
- MeSH
- Dextrans MeSH
- Microscopy, Electron MeSH
- Diabetes Mellitus, Experimental pathology MeSH
- Phantoms, Imaging MeSH
- Contrast Media MeSH
- Blood Glucose analysis MeSH
- Rats MeSH
- Magnetic Resonance Imaging methods MeSH
- Magnetite Nanoparticles MeSH
- Ferrosoferric Oxide MeSH
- Oxides MeSH
- Rats, Inbred Lew MeSH
- Islets of Langerhans Transplantation * MeSH
- Iron MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Dextrans MeSH
- ferumoxides MeSH Browser
- Contrast Media MeSH
- Blood Glucose MeSH
- Magnetite Nanoparticles MeSH
- Ferrosoferric Oxide MeSH
- Oxides MeSH
- Iron MeSH
A promising treatment method for type 1 diabetes mellitus is transplantation of pancreatic islets containing beta-cells. The aim of this study was to develop an MR technique to monitor the distribution and fate of transplanted pancreatic islets in an animal model. Twenty-five hundred purified and magnetically labeled islets were transplanted through the portal vein into the liver of experimental rats. The animals were scanned using a MR 4.7-T scanner. The labeled pancreatic islets were clearly visualized in the liver in both diabetic and healthy rats as hypointense areas on T2*-weighted MR images during the entire measurement period. Transmission electron microscopy confirmed the presence of iron-oxide nanoparticles inside the cells of the pancreatic islets. A significant decrease in blood glucose levels in diabetic rats was observed; normal glycemia was reached 1 week after transplantation. This study, therefore, represents a promising step toward possible clinical application in human medicine.
References provided by Crossref.org
Cationic fluorinated micelles for cell labeling and 19F-MR imaging
Pre-Microporation Improves Outcome of Pancreatic Islet Labelling for Optical and 19F MR Imaging
Imaging the Beta-cell mass: why and how
