Permeability and microstructure of model stratum corneum lipid membranes containing ceramides with long (C16) and very long (C24) acyl chains
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
28363088
DOI
10.1016/j.bpc.2017.03.004
PII: S0301-4622(16)30463-X
Knihovny.cz E-resources
- MeSH
- Ceramides chemistry MeSH
- Cholesterol MeSH
- X-Ray Diffraction MeSH
- Epidermis chemistry metabolism MeSH
- Cholesterol Esters MeSH
- Humans MeSH
- Fatty Acids MeSH
- Membrane Lipids chemistry MeSH
- Microscopy, Atomic Force MeSH
- Permeability MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ceramides MeSH
- Cholesterol MeSH
- cholesteryl sulfate MeSH Browser
- Cholesterol Esters MeSH
- lignoceric acid MeSH Browser
- Fatty Acids MeSH
- Membrane Lipids MeSH
The Stratum corneum (SC) prevents water loss from the body and absorption of chemicals. SC intercellular spaces contain ceramides (Cer), free fatty acids (FFA), cholesterol (Chol) and cholesteryl sulfate (CholS). Cer with "very long" acyl chains (for example, N-lignoceroyl-sphingosine, CerNS24) are important for skin barrier function, whereas increased levels of "long" acyl Cer (for example, N-palmitoyl-sphingosine, CerNS16) occur in patients suffering from atopic eczema or psoriasis. We studied the impact of the replacement of CerNS24 by CerNS16 on the barrier properties and microstructure of model SC lipid membranes composed of Cer/FFA/Chol/CholS. Membranes containing the long CerNS16 were significantly more permeable to water (by 38-53%), theophylline (by 50-55%) and indomethacin (by 83-120%) than those containing the very long CerNS24 (either with lignoceric acid or a mixture of long to very long chain FFA). Langmuir monolayers with CerNS24 were more condensed than with CerNS16 and atomic force microscopy showed differences in domain formation. X-ray powder diffraction revealed that CerNS24-based membranes formed one lamellar phase and separated Chol, whereas the CerNS16-based membranes formed up to three phases and Chol. These results suggest that replacement of CerNS24 by CerNS16 has a direct negative impact on membrane structure and permeability.
References provided by Crossref.org
Lysosphingolipids in ceramide-deficient skin lipid models
Polymorphism, Nanostructures, and Barrier Properties of Ceramide-Based Lipid Films
Behavior of 1-Deoxy-, 3-Deoxy- and N-Methyl-Ceramides in Skin Barrier Lipid Models
