Extracellularly distributed collagen and chondrocytes seeded in gelatine and poly-ɛ-caprolactone scaffolds are visualized by two-photon excitation microscopy (TPEM) and second-harmonic generation (SHG) imaging in both forward and backward nondescanned modes. Joint application of TPEM and SHG imaging in combination with stereological measurements of collagen enables us not only to take high-resolution 3-D images, but also to quantitatively analyze the collagen volume and a spatial arrangement of cell-collagen-scaffold systems, which was previously impossible. This novel approach represents a powerful tool for the analysis of collagen-containing scaffolds with applications in cartilage tissue engineering.
- MeSH
- chondrocyty cytologie metabolismus transplantace MeSH
- fluorescenční mikroskopie metody MeSH
- interpretace obrazu počítačem metody MeSH
- kolagen metabolismus ultrastruktura MeSH
- králíci MeSH
- kultivované buňky MeSH
- nelineární dynamika MeSH
- tkáňové podpůrné struktury MeSH
- zobrazování trojrozměrné metody MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Statins have revolutionized the treatment of hypercholesterolemia due to their ability to inhibit cholesterol biosynthesis. Their immunomodulatory and anti-inflammatory effects and positive effects on the treatment of atherosclerosis and its complications are well known. Here, we describe the effects of statins on the treatment of presbycusis in C57BL/6J mice. In this strain with accelerated aging, we demonstrate that animals treated with atorvastatin (10mg/kg per day in chow diet) for 2 months showed larger amplitudes of distortion product otoacoustic emissions (DPOAE) than did the non-treated control group. This finding indicates a better survival of outer hair cell function in the inner ear of C57BL/6J mice. The observed decreased expression of intercellular and vascular adhesion molecules in the aortic wall of atorvastatin-treated animals suggests that reducing endothelial inflammatory effects may contribute to the positive effect of atorvastatin on the amplitudes of DPOAE by influencing the blood supply to the inner ear. No such beneficial effect of statins was found in apoE(-/-) mice treated with atorvastatin under the same conditions. Our results suggest that statins could also slow down the age-related deterioration of hearing in man.
- MeSH
- akustická stimulace MeSH
- apolipoproteiny E nedostatek MeSH
- časové faktory MeSH
- exprese genu genetika MeSH
- financování organizované MeSH
- kyseliny heptylové terapeutické užití MeSH
- mezibuněčná adhezivní molekula-1 metabolismus MeSH
- myši inbrední C57BL MeSH
- myši knockoutované MeSH
- myši MeSH
- otoakustické emise spontánní účinky léků MeSH
- potrava speciální MeSH
- presbyakuze dietoterapie patologie prevence a kontrola MeSH
- pyrroly terapeutické užití MeSH
- receptory vasopresinů metabolismus MeSH
- sluchový práh fyziologie účinky léků MeSH
- stárnutí MeSH
- statiny terapeutické užití MeSH
- vnitřní ucho patofyziologie MeSH
- vztah dávky záření a odpovědi MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
