Autologous cell therapy (ACT) is a new therapeutic approach for diabetic patients with no-option chronic limb-threatening ischemia (NO-CLTI). The aim of our study was to quantify cell populations of cell therapy products (CTPs) obtained by three different isolation methods and to correlate their numbers with changes in transcutaneous oxygen pressure (TcPO2). CTPs were separated either from stimulated peripheral blood (PB) (n = 11) or harvested from bone marrow (BM) processed either by Harvest SmartPReP2 (n = 50) or sedimented with succinate gelatin (n = 29). The clinical effect was evaluated by the change in TcPO2 after 1, 3 and 6 months. TcPO2 increased significantly in all three methods at each time point in comparison with baseline values (p < .01) with no significant difference among them. There was no correlation between the change in TcPO2 and the size of injected cell populations. We only observed a weak correlation between the number of injected white blood cells (WBC) and an increase in TcPO2 at 1 and 3 months. Our study showed that all three isolation methods of ACT were similarly relatively efficient in the treatment of NO-CLTI. We observed no correlation of TcPO2 increase with the number of injected monocytes, lymphocytes or CD34+. We observed a weak correlation between TcPO2 increase and the number of injected WBCs.
- MeSH
- autologní transplantace metody MeSH
- buněčná a tkáňová terapie * metody MeSH
- chronická kritická ischemie končetin * terapie diagnóza chirurgie MeSH
- ischemie * terapie etiologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- transkutánní měření krevních plynů metody MeSH
- výsledek terapie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
BACKGROUND: Diabetic patients (DPs) with foot ulcers can receive autologous cell therapy (ACT) as a last therapeutic option. Even DPs who have undergone organ transplantation and are using immunosuppressive (IS) drugs can be treated by ACT. The aim of our study was to analyze the effects of IS drugs on the characteristics of bone marrow-derived stem cells (BM-MSCs). METHODS: The cells were isolated from the bone marrow of DPs, cultivated for 14-18 days, and phenotypically characterized using flow cytometry. These precursor cells were cultured in the presence of various IS drugs. The impact of IS drugs on metabolic activity was measured using a WST-1 assay, and the expression of genes for immunoregulatory molecules was detected through RT-PCR. Cell death was analyzed through the use of flow cytometry, and the production of cytokines was determined by ELISA. RESULTS: The mononuclear fraction of cultured cells contained mesenchymal stem cells (CD45-CD73+CD90+CD105+), myeloid angiogenic cells (CD45+CD146-), and endothelial colony-forming cells (CD45-CD146+). IS drugs inhibited metabolic activity, the expression of genes for immunoregulatory molecules, the production of cytokines, and the viability of the cells. CONCLUSIONS: The results indicate that IS drugs in a dose-dependent manner had a negative impact on the properties of BM-MSCs used to treat ischemic diabetic foot ulcers, and that these drugs could affect the therapeutic potential of BM-MSCs.
- Publikační typ
- časopisecké články MeSH
Diabetes mellitus představuje onemocnění, které je často spojeno s mnohočetnými komplikacemi, a to zejména vlivem dlouhodobě neuspokojivé kompenzace. Ve spojitosti s diabetem je však málokdy zmiňováno riziko rozvoje sexuálních dysfunkcí, které postihují muže i ženy (1). Sexuální komplikace nenarušují pouze sociální a pohlavní život pacientů, ale jsou mnohdy prvním příznakem dalších zdravotních obtíží. Z těchto důvodů by u lidí s diabetem měl být včasný záchyt a správná léčba sexuálních dysfunkcí předmětem zájmu lékařů pečujících o diabetiky.
Diabetes mellitus is a disease associated with multiple complications due to ineffective diabetes management in the early period after diagnosis and especially in the long term. However, the risk of developing sexual dysfunctions, which affect both men and women, is rarely mentioned in association with diabetes (1). Sexual complications not only disrupt with the social and sexual life of patients, but are often the first symptom of other health problems. For these reasons, in people with diabetes, early detection and proper treatment of sexual dysfunctions should be of concern physicians caring for diabetic patients.
- MeSH
- erektilní dysfunkce diagnóza etiologie terapie MeSH
- hypogonadismus etiologie patofyziologie terapie MeSH
- inhibitory fosfodiesterasy 5 terapeutické užití MeSH
- komplikace diabetu * diagnóza terapie MeSH
- lidé MeSH
- předčasná ejakulace diagnóza etiologie terapie MeSH
- sexuální dysfunkce fyziologická * diagnóza etiologie terapie MeSH
- sexuální dysfunkce psychické diagnóza etiologie terapie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
