Cerebrospinal fluid (CSF) samples (n=50) from patients with neurological disease (bacterial infection, viral infection, neuroborreliosis and multiple sclerosis) were analysed to characterize cell populations by fluorescent immunocytometry with the CD-Sapphire haematology analyser. Reagent combinations applied to all CSF samples comprised CD3/CD19/HLA-DR and CD4/CD8, with some being further analysed using CD3/CD4, CD3/CD16 and CD3/CD25 protocols. Of the 50 samples, 11 were excluded because of high proportions of nonviable cells (n=2) or insufficient cell numbers (n=9). Apart from bacterial infection with granulocytosis, all diagnostic groups showed high proportions (51.4-77.0%) of CD3+ T cells. There was a modest association between T-cell and B-cell counts, but absolute B-cell numbers exceeded 5 cells/microl in only 7/39 cases (neuroborreliosis, n=6; bacterial meningitis, n=1). CD3/Ia antigen (activation) co-expression was low and only exceeded 5% in 7/39 samples with no diagnostic correlation. Primary CD4+ and CD8+ T-cell subsets showed similar quantitative trends and CD4/CD8 co-analysis revealed the presence in all diagnostic groups (neuroborreliosis and multiple sclerosis in particular) of a CD4+CD8int fraction that was predominantly CD3+ and CD16- and had a morphological profile consistent with small lymphoid cells. Supplementary CD-Sapphire cellular immunological analysis of most CSF samples is feasible using the procedure detailed in this communication.
- MeSH
- cytologické techniky metody MeSH
- granulocyty cytologie patologie MeSH
- intrakraniální krvácení MeSH
- lidé MeSH
- lymfocyty cytologie patologie MeSH
- mononukleární fagocytární systém cytologie patologie MeSH
- mozkomíšní mok cytologie chemie mikrobiologie MeSH
- nádorové cirkulující buňky klasifikace patologie MeSH
- patologické procesy etiologie klasifikace patologie MeSH
- Check Tag
- lidé MeSH
- MeSH
- eozinofily cytologie patologie MeSH
- glukosa metabolismus MeSH
- hematoencefalická bariéra cytologie chemie patologie MeSH
- intrakraniální krvácení metabolismus MeSH
- lidé MeSH
- mozkomíšní mok cytologie chemie MeSH
- nemoci centrálního nervového systému MeSH
- neutrofily cytologie patologie MeSH
- prekurzorové buňky monocytů-makrofágů cytologie patologie MeSH
- proteiny v mozkomíšním moku diagnostické užití MeSH
- referenční hodnoty MeSH
- syndrom systémové zánětlivé reakce MeSH
- Check Tag
- lidé MeSH
The levels of prealbumin (PAB, transthyretin) were determined and evaluated in the cerebrospinal fluid (CSF) and serum in various subgroups of the multiple sclerosis (MS) patients. In severely disabled patients, serum PAB was elevated more frequently. CSF and serum PAB concentrations were higher in treated than in nontreated patients; the values above the upper reference limits were also more frequently found in treated patients. PAB index showed a tendency to decrease during the course of the disease. The routine determination of PAB in CSF and serum is, therefore, recommended to be recognized in MS patients as a substantial clinical value and, thus, be comprised, including also immunoglobulins and other parameters, into the spectrum of characteristics in Protein Panel.
- MeSH
- eozinofily imunologie MeSH
- glukosa MeSH
- krevní proteiny MeSH
- kyselina mléčná MeSH
- lidé MeSH
- meningitida diagnóza imunologie MeSH
- počet leukocytů MeSH
- T-lymfocyty pomocné-indukující imunologie MeSH
- T-lymfocyty imunologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- kazuistiky MeSH
A routine diagnostic procedure of cryptococcal meningitis using Alcian Blue and Nuclear Fast Red staining is described in a group of 16 patients. Cerebrospinal fluid findings, including clinical cytology, routine biochemistry and protein fractions, are presented.
