BACKGROUND: The main mechanisms causing high-level resistance to fluoroquinolones (FQ) are encoded chromosomally; that includes mutations in genes coding DNA-gyrase, but overexpression of efflux pumps contributes to increased minimum inhibitory concentration (MIC) of FQ as well. However, genes responsible for FQ-resistance may be harboured in transferable/conjugative plasmids. For some time, there was an assumption that resistance to FQ cannot be transferable in conjugation due to their synthetic origin, until 1998, when plasmid-mediated resistance transmission in Klebsiella pneumoniae was proved. We aimed to detect the occurrence of transferable FQ-resistance among Gram- negative bacteria isolated from patients in Czech and Slovak hospitals. METHODS: In this study, we tested 236 clinical isolates of Gram-negative bacteria for transferable resistance. Among relevant isolates we performed PCR detection of transferable fluoroquinolone genes (qnr). RESULTS: We have observed transfer of determinants of cephalosporin-resistance, aminoglycoside resistance as well as FQ-resistance (in 10 cases; 4.24%) not only intra-species but inter-species too. The presence of qnr gene was detected in two isolates of forty tested (5%). We have also observed that determinants of cephalosporin-resistance and aminoglycoside-resistance were linked to those of FQ-resistance and were transferred en block in conjugation. CONCLUSION: We have proved that resistance to fluoroquinolones can be transferred horizontally via conjugation among Gram-negative bacteria of different species and is associated with resistance to other antibiotics.
- MeSH
- antibakteriální látky farmakologie terapeutické užití MeSH
- Enterobacteriaceae účinky léků izolace a purifikace MeSH
- fluorochinolony farmakologie terapeutické užití MeSH
- lidé MeSH
- mnohočetná bakteriální léková rezistence účinky léků genetika MeSH
- polymerázová řetězová reakce MeSH
- Pseudomonas aeruginosa účinky léků izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Slovenská republika MeSH
- MeSH
- antibakteriální látky MeSH
- bakteriemie farmakoterapie mikrobiologie MeSH
- gramnegativní bakterie izolace a purifikace účinky léků MeSH
- grampozitivní bakterie izolace a purifikace účinky léků MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- mnohočetná bakteriální léková rezistence MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
Autori demonštrovali transferabilitu génov antibiotickej rezistencie u nozokomiálnych kmeňovKlebsiella pneumoniae, izolovaných od novorodencov v Detskej fakultnej nemocnici s poliklinikouv Bratislave, rezistentných na cefotaxím, ceftazidím a aztreonam. Determinanty rezistencie nab-laktámové antibiotiká (karbenicilín, cefaloridín, cefotaxím, ceftazidím a aztreonam) boli prenosnéna recipientné kmene Escherichia coli K-12 a Proteus mirabilis P-38. Prenos determinantov rezis-tencie z donorných kmeňov bol dokázaný analýzou spektra rezistencie u transkonjugantných klo-nov recipientných kmeňov metódou nepriamej selekcie. Pomocou dvojdiskového difúzneho testubola u týchto kmeňov zistená schopnosť produkovať ESBL (b-laktamázy s rozšíreným spektromúčinku). Synergia medzi klavulanátom a cefotaxímom, klavulanátom a ceftazidímom, alebo klavu-lanátom a aztreonamom indikovala produkciu ESBL týmito kmeňmi.
The authors demonstrated the transferability of antibiotic resistance genes in nosocomial strainsof Klebsiella pneumoniae, isolated from newborn children at the Paediatric University Hospital inBratislava. Strains were resistant to cefotaxime, ceftazidime and aztreonam. The determinants ofresistance (carbenicillin, cephaloridine, cefotaxime, ceftazidime and aztreonam) were transferredto recipient strains of Escherichia coli K-12 and Proteus mirabilis P-38. The transfer of resistancedeterminant from donor strains was demonstrated by the analysis of the resistance spectrum intransconjugant clones of recipient strains by the method of indirect selection. The ability ofproduction of extended spectrum b-lactamases (ESBL) was demonstrated by the double disc diffu-sion test. Synergy between clavulanate and cefotaxime, clavulanate and ceftazidime and/or clavu-lanate and aztreonam indicated production of ESBL by these strains.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriofágy genetika MeSH
- beta-laktamasy genetika MeSH
- beta-laktamová rezistence MeSH
- imipenem farmakologie MeSH
- laktamy MeSH
- lidé MeSH
- Pseudomonas aeruginosa genetika izolace a purifikace účinky léků MeSH
- transdukce genetická MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
