Our study aimed to investigate subacute exposure to alcohol in relation to bone microstructure of mice. Animals from experimental (E) group drank a solution composed of 15 % ethanol and water for 14 days (one remodeling cycle), while those from control (C) group drank only water. In the compact bone of E group, decreased bone formation and increased porosity were observed which corresponds with lower levels of serum alkaline phosphatase and glutathione. Alcohol significantly increased sizes of primary osteon's vascular canals and decreased those of secondary osteons, Haversian canals. Relative bone volume, bone mineral density (BMD), relative bone volume without marrow cavity were also lower in E group. On the contrary, trabecular bone microstructure did not differ significantly between E and C groups. Liver function test showed higher levels of alanine aminotransferase, aspartate aminotransferase in alcohol-fed mice. Serum calcium, phosphate were significantly lower in E group. According to our study, only changes in compact bone microstructure of mice following one remodeling cycle were observed due to both direct and indirect effects of alcohol.
- MeSH
- ethanol aplikace a dávkování toxicita MeSH
- kostní denzita účinky léků fyziologie MeSH
- kostní matrix diagnostické zobrazování účinky léků fyziologie MeSH
- myši MeSH
- zobrazování trojrozměrné metody MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- biologické markery analýza MeSH
- fraktury kostí epidemiologie klasifikace MeSH
- kostní denzita genetika MeSH
- lidé MeSH
- osteoporóza diagnóza genetika MeSH
- polymerázová řetězová reakce metody MeSH
- polymorfismus genetický genetika MeSH
- postmenopauzální osteoporóza epidemiologie genetika MeSH
- receptory kalcitoninu genetika MeSH
- receptory kalcitriolu genetika MeSH
- receptory pro estrogeny genetika MeSH
- remodelace kosti genetika MeSH
- věkové faktory MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- přehledy MeSH
Differences in microscopic structure of the femur between 1-month-old transgenic rabbits carrying the hFVIII gene and non-transgenic rabbits were investigated. Bone microstructure was evaluated from the point of view of qualitative and quantitative histological characteristics. We identified fibrolamellar bone tissue only in the transgenic animals. Measured values for area, perimeter of the Haversian canals and minimum diameter of the primary osteons' vascular canals were higher in 1-month-old transgenic individuals (P < 0.05; P < 0.001). We also observed lower concentrations of Ca, P, K, solids, and total mineral content in femora of transgenic rabbits. A statistically significant difference was observed for the concentration of Ca (P < 0.05). Our results indicate evident changes in both qualitative and quantitative histological characteristics of the femur, which result especially in better blood supply and slightly reduced mineralization process in 1-month-old transgenic rabbits.
- MeSH
- faktor VIII genetika MeSH
- femur cytologie MeSH
- fyziologická kalcifikace MeSH
- geneticky modifikovaná zvířata MeSH
- kostní denzita MeSH
- králíci MeSH
- lidé MeSH
- mléčné bílkoviny genetika MeSH
- novorozená zvířata MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
