Our study aimed to investigate subacute exposure to alcohol in relation to bone microstructure of mice. Animals from experimental (E) group drank a solution composed of 15 % ethanol and water for 14 days (one remodeling cycle), while those from control (C) group drank only water. In the compact bone of E group, decreased bone formation and increased porosity were observed which corresponds with lower levels of serum alkaline phosphatase and glutathione. Alcohol significantly increased sizes of primary osteon's vascular canals and decreased those of secondary osteons, Haversian canals. Relative bone volume, bone mineral density (BMD), relative bone volume without marrow cavity were also lower in E group. On the contrary, trabecular bone microstructure did not differ significantly between E and C groups. Liver function test showed higher levels of alanine aminotransferase, aspartate aminotransferase in alcohol-fed mice. Serum calcium, phosphate were significantly lower in E group. According to our study, only changes in compact bone microstructure of mice following one remodeling cycle were observed due to both direct and indirect effects of alcohol.
- MeSH
- ethanol aplikace a dávkování toxicita MeSH
- kostní denzita účinky léků fyziologie MeSH
- kostní matrix diagnostické zobrazování účinky léků fyziologie MeSH
- myši MeSH
- zobrazování trojrozměrné metody MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Prospective, controlled clinical trials in postmenopausal osteoporosis typically compare effects of an active drug with placebo in addition to vitamin D and calcium supplementation in both treatment arms. While clinical benefits are documented, the effect of this supplementation in the placebo arm and in clinical practice on bone material composition properties is unknown. The purpose of the present study was to evaluate these bone quality indices (specifically mineral/matrix, nanoporosity, glycosaminoglycan content, mineral maturity/crystallinity, and pyridinoline content) in patients that either received long-term vitamin D (400-1200IU) and calcium (1.0-1.5g) supplementation, or did not. We have analyzed by Raman microspectroscopy the bone forming trabecular surfaces of iliac crest in pre-treatment samples of a teriparatide study and the endpoint biopsies of the control arm obtained from the HORIZON trial. In general, the mineral/matrix ratio and the glycosaminoglycan (GAG) content was higher while nanoporosity, (a surrogate for tissue water content), the mineral maturity/crystallinity (MMC) and the pyridinoline (Pyd) content was lower in patients without long-term supplementation. Moreover, all indices were significantly dependent on tissue age. In conclusion, vitamin D and calcium supplementation is associated with altered mineral and organic matrix properties.
- MeSH
- aminokyseliny metabolismus MeSH
- analýza rozptylu MeSH
- fyziologická kalcifikace účinky léků MeSH
- glykosaminoglykany metabolismus MeSH
- kostní matrix účinky léků metabolismus MeSH
- lidé MeSH
- nanočástice chemie MeSH
- poréznost MeSH
- postmenopauzální osteoporóza farmakoterapie patofyziologie MeSH
- potravní doplňky * MeSH
- Ramanova spektroskopie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- vápník farmakologie terapeutické užití MeSH
- vitamin D farmakologie terapeutické užití MeSH
- Check Tag
- lidé MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- chrom * škodlivé účinky MeSH
- elektronová mikroskopie MeSH
- kostní matrix účinky léků MeSH
- modely nemocí na zvířatech MeSH
- parodont * účinky léků MeSH
- parodontitida chemicky indukované MeSH
- potkani Sprague-Dawley * anatomie a histologie MeSH
- resorpce alveolární kosti chemicky indukované MeSH
- ztráta zubů chemicky indukované MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
