Although the identification of animal species and muscles have been reported previously, no studies have been found on the use of NIR spectroscopy to identify individual animals from the analysis of commercial meat cuts. The aim of this study was to evaluate the use of a portable near infrared (NIR) instrument combined with classical chemometrics methods [principal component analysis (PCA) and partial least squares discriminant analysis PLS-DA)] to identify the origin of individual goat animals using the spectral signature of their commercial cut. Samples were collected from several carcasses (6 commercial cuts x 24 animals) sourced from a commercial abattoir in Queensland (Australia). The NIR spectra of the samples were collected using a portable NIR instrument in the wavelength range between 950 and 1600 nm. Overall, the PLS-DA models correctly classify 82% and 79% of the individual goat samples using either the goat rack or loin cut samples, respectively. The study demonstrated that NIR spectroscopy was able to identify individual goat animals based on the spectra properties of some of the commercial cut samples analysed (e.g. loin and rack). These results showed the potential of this technique to identify individual animals as an alternative to other laboratory methods and techniques commonly used in meat traceability.
- Publikační typ
- časopisecké články MeSH
Allelic and genotypic distribution of polymorphisms in diacylglycerol acyltransferase 1 (DGAT1), fatty acid binding protein 4 (FABP4), fatty acid synthase (FASN), and peroxisome proliferator-activated receptor-γ coactivator-1α (PPARGC1A) genes were assessed in 679 Fleckvieh bulls. Single-locus genotype effects and the combined effect of the two polymorphisms within the FASN gene were evaluated for association with the intramuscular fat content and fatty acid profile determined in muscle and subcutaneous fat. The FASN (g.16024G>A) and FASN (g.17924A>G) polymorphisms were significantly associated mainly with C14:0, C16:0, and C18:1 n-9 concentrations as well as with the atherogenic index. The proportion of explained phenotypic variation markedly increased when the effect of a combination of the two polymorphisms within the FASN gene was tested, with the highest values of 8.6% and 14.8%, respectively, observed for C14:0 in muscle and subcutaneous fat. With a focus on improving the fatty acid composition of beef, the results of this study provide valuable information about the markers applicable in marker-assisted selection.
- MeSH
- alely MeSH
- ateroskleróza MeSH
- diacylglycerol-O-acyltransferasa genetika metabolismus MeSH
- druhová specificita MeSH
- fenotyp * MeSH
- genotyp * MeSH
- jednonukleotidový polymorfismus MeSH
- lidé MeSH
- maso analýza MeSH
- mastné kyseliny metabolismus MeSH
- podkožní tuk metabolismus MeSH
- polymorfismus genetický * MeSH
- proteiny vázající mastné kyseliny genetika metabolismus MeSH
- skot MeSH
- svaly metabolismus MeSH
- syntázy mastných kyselin genetika metabolismus MeSH
- transkripční faktory genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The objective of the study was to investigate the effects of sex and slaughter age on the fatty acid (FA) composition and stearoyl-CoA desaturase gene expression in muscle and adipose tissue. Twenty-four Charolais × Simmental crossbred bulls and heifers were raised under similar conditions and slaughtered at 14 or 18 months of age. The total amount of FA in muscle increased markedly in older animals with higher contents of monounsaturated FA (MUFA) in heifers than bulls. The proportions of MUFA and desaturation indices were higher in heifers, whereas polyunsaturated FA were higher in bulls in both muscle and subcutaneous adipose tissue. The results of this study demonstrated sex-dependent differences in the FA composition of muscle and subcutaneous adipose tissue from cattle slaughtered at different ages. The expression of the stearoyl-CoA desaturase gene was higher in the adipose tissue of heifers compared to bulls, and its variation partly contributed to sex- and age-differences in the FA composition of bovine adipose tissue.
- MeSH
- kosterní svaly chemie enzymologie MeSH
- kyseliny mastné mononenasycené analýza MeSH
- mastné kyseliny analýza MeSH
- messenger RNA genetika metabolismus MeSH
- nenasycené mastné kyseliny analýza MeSH
- podkožní tuk chemie enzymologie MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- regulace genové exprese MeSH
- sexuální faktory MeSH
- skot MeSH
- stearyl-CoA-desaturasa genetika metabolismus MeSH
- věkové faktory MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
The previously reported genetic polymorphisms of the stearoyl-CoA desaturase (SCD1) and sterol regulatory element binding protein-1 (SREBP-1) genes were investigated in Fleckvieh bulls using the PCR-RFLP and AS-PCR methods, respectively. The genomic DNA was obtained from a total of 370 bulls. The frequencies of alleles A and V of the single nucleotide polymorphism in exon 5 of the SCD1 gene (SNP 878C>T) were 0.555 and 0.445, respectively. In the 84-bp Ins/Del polymorphism in intron 5 of the SREBP-1 gene, the frequency of the L allele (insertion) was markedly higher (0.920) than that of the S allele (deletion; 0.080). Fatty acid profile was determined in a total of 367 samples of muscle fat (MSF) and 150 samples of subcutaneous fat (SCF). The AA genotype of SCD1 polymorphism showed a lower content of C18:0 (P<0.01) and higher contents of C14:1 cis-9 (P<0.001) and C18:1 cis-9 (P<0.05) in MSF compared to the VV genotype. As a result, the bulls with genotypes AA or AV had lower SFA (P<0.01), higher MUFA (P<0.05) and higher MUFA/SFA (P<0.01) than VV animals. The results obtained for SCF were similar. The SREBP-1 polymorphism was associated with a higher content of C14:1 cis-9 (P<0.01) in the LS compared to LL genotype in SCF. The results of this study demonstrated the existence of the polymorphisms in the SCD1 and SREBP-1 genes in the population of Fleckvieh cattle and their associations with the concentrations of several MSF and SCF fatty acids.
- MeSH
- DNA analýza MeSH
- exony MeSH
- frekvence genu MeSH
- genom MeSH
- genotyp MeSH
- introny MeSH
- jednonukleotidový polymorfismus MeSH
- kosterní svaly chemie MeSH
- kyseliny mastné mononenasycené analýza MeSH
- mastné kyseliny analýza genetika MeSH
- podkožní tuk chemie MeSH
- polymerázová řetězová reakce metody MeSH
- protein SREBP1 genetika MeSH
- skot MeSH
- stearyl-CoA-desaturasa genetika MeSH
- uhlík MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
