Cruciform structures are preferential targets for many architectural and regulatory proteins, as well as a number of DNA binding proteins with weak sequence specificity. Some of these proteins are also capable of inducing the formation of cruciform structures upon DNA binding. In this paper we analyzed the amino acid composition of eighteen cruciform binding proteins of Homo sapiens. Comparison with general amino acid frequencies in all human proteins revealed unique differences, with notable enrichment for lysine and serine and/or depletion for alanine, glycine, glutamine, arginine, tyrosine and tryptophan residues. Based on bootstrap resampling and fuzzy cluster analysis, multiple molecular mechanisms of interaction with cruciform DNA structures could be suggested, including those involved in DNA repair, transcription and chromatin regulation. The proteins DEK, HMGB1 and TOP1 in particular formed a very distinctive group. Nonetheless, a strong interaction network connecting nearly all the cruciform binding proteins studied was demonstrated. Data reported here will be very useful for future prediction of new cruciform binding proteins or even construction of predictive tool/web-based application.
- MeSH
- aminokyseliny chemie MeSH
- chromatin MeSH
- chromozomální proteiny, nehistonové chemie MeSH
- DNA vazebné proteiny chemie MeSH
- DNA-topoisomerasy I chemie MeSH
- konformace nukleové kyseliny MeSH
- lidé MeSH
- onkogenní proteiny chemie MeSH
- protein HMGB1 chemie MeSH
- proteiny vázající poly-ADP-ribosu chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Publikační typ
- abstrakt z konference MeSH
- MeSH
- kardiostimulace umělá MeSH
- kardiostimulátor MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- kongresy MeSH
- MeSH
- dospělí MeSH
- hemodynamika MeSH
- kardiochirurgické výkony MeSH
- koronární nemoc diagnóza chirurgie MeSH
- lidé středního věku MeSH
- lidé MeSH
- myokard MeSH
- radioisotopová scintigrafie metody statistika a číselné údaje MeSH
- revaskularizace myokardu MeSH
- senioři MeSH
- srdce fyziologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
