Mitochondrial retrograde signaling is a pathway of communication from mitochondria to the nucleus. Recently, natural mitochondrial genome (mtDNA) polymorphisms (haplogroups) received increasing attention in the pathophysiology of human common diseases. However, retrograde effects of mtDNA variants on such traits are difficult to study in humans. The conplastic strains represent key animal models to elucidate regulatory roles of mtDNA haplogroups on defined nuclear genome background. To analyze the relationship between mtDNA variants and cardiometabolic traits, we derived a set of rat conplastic strains (SHR-mtBN, SHR-mtF344 and SHR-mtLEW), harboring all major mtDNA haplotypes present in common inbred strains on the nuclear background of the spontaneously hypertensive rat (SHR). The BN, F344 and LEW mtDNA differ from the SHR in multiple amino acid substitutions in protein coding genes and also in variants of tRNA and rRNA genes. Different mtDNA haplotypes were found to predispose to various sets of cardiometabolic phenotypes which provided evidence for significant retrograde effects of mtDNA in the SHR. In the future, these animals could be used to decipher individual biochemical components involved in the retrograde signaling.
- MeSH
- fenotyp MeSH
- kardiovaskulární nemoci * metabolismus MeSH
- krysa rodu rattus MeSH
- mitochondriální DNA * genetika MeSH
- mitochondrie metabolismus MeSH
- potkani inbrední F344 MeSH
- potkani inbrední SHR MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Age and sex play an essential role in the cardiac tolerance to ischemia-reperfusion injury: cardiac resistance significantly decreases during postnatal maturation and the female heart is more tolerant than the male myocardium. It is widely accepted that mitochondrial dysfunction, and particularly mitochondrial permeability transition pore (MPTP) opening, plays a major role in determining the extent of cardiac ischemia-reperfusion injury. We have observed that the MPTP sensitivity to the calcium load differs in mitochondria isolated from neonatal and adult myocardium, as well as from adult male and female hearts. Neonatal and female mitochondria are more resistant both in the extent and in the rate of mitochondrial swelling induced by high calcium concentration. Our data further suggest that age- and sex-dependent specificity of the MPTP is not the result of different amounts of ATP synthase and cyclophilin D: neonatal and adult hearts, similarly as the male and female hearts, contain comparable amounts of MPTP and its regulatory protein cyclophilin D. We can speculate that the lower sensitivity of MPTP to the calcium-induced swelling may be related to the higher ischemic tolerance of both neonatal and female myocardium.
- MeSH
- lidé MeSH
- pohlavní dimorfismus * MeSH
- reperfuzní poškození myokardu metabolismus patologie patofyziologie MeSH
- srdce * patofyziologie MeSH
- srdeční mitochondrie metabolismus patologie MeSH
- transportní proteiny mitochondriální membrány metabolismus MeSH
- vápník metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- MeSH
- financování organizované MeSH
- Publikační typ
- abstrakty MeSH
- MeSH
- financování organizované MeSH
- Publikační typ
- abstrakty MeSH
- MeSH
- financování organizované MeSH
- Publikační typ
- abstrakty MeSH
- MeSH
- finanční podpora výzkumu jako téma MeSH
- Publikační typ
- abstrakty MeSH
Child health care is an integral part and practically the most important part of the health care delivery system in today's society. This article discusses the special characteristics of child health care within the total population care effort, with emphasis on the prevention, diagnosis and the treatment of physical and psychological ailments of infancy and childhood.
- MeSH
- dítě MeSH
- genetické poradenství MeSH
- kojenec MeSH
- lidé MeSH
- mezinárodní agentury MeSH
- pediatrie MeSH
- poskytování zdravotní péče organizace a řízení MeSH
- primární zdravotní péče organizace a řízení MeSH
- regionální zdravotnické plánování MeSH
- školní zdravotnické služby organizace a řízení MeSH
- služby preventivní péče organizace a řízení MeSH
- služby zdravotní péče o dítě organizace a řízení MeSH
- zdravotnické služby - potřeby a požadavky MeSH
- zneužívání zdravotnických služeb MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Československo MeSH
