- MeSH
- analýza potravin MeSH
- biologická dostupnost MeSH
- cinnamáty MeSH
- flavonoidy MeSH
- flavonoly MeSH
- flavony MeSH
- isoflavony MeSH
- kyselina benzoová MeSH
- lidé MeSH
- lignany MeSH
- manipulace s potravinami MeSH
- ovoce * MeSH
- polyfenoly * fyziologie chemie metabolismus MeSH
- potraviny a nápoje * MeSH
- stilbeny MeSH
- zelenina * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Klíčová slova
- pozitivní účinky, negativní účinky,
- MeSH
- antikarcinogenní látky MeSH
- antioxidancia * fyziologie MeSH
- bezpečnost * MeSH
- fytoestrogeny škodlivé účinky MeSH
- karcinogeny MeSH
- kardiovaskulární nemoci prevence a kontrola MeSH
- lékové interakce MeSH
- lidé MeSH
- mutageny škodlivé účinky MeSH
- polyfenoly * fyziologie metabolismus škodlivé účinky terapeutické užití MeSH
- potravní doplňky škodlivé účinky MeSH
- rostlinné extrakty MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
Every day, genomes are affected by genotoxic factors that create multiple DNA lesions. Several DNA repair systems have evolved to counteract the deleterious effects of DNA damage. These systems include a set of DNA repair mechanisms, damage tolerance processes, and activation of cell-cycle checkpoints. This study describes selected confocal microscopy techniques that investigate DNA damage-related nuclear events after UVA- and γ-irradiation and compare the DNA damage response (DDR) induced by the two experimental approaches. In both cases, we observed induction of the nucleotide excision repair (NER) pathway and formation of localized double-strand breaks (DSBs). This was confirmed by analysis of cyclobutane pyrimidine dimers (CPDs) in the DNA lesions and by increased levels of γH2AX and 53BP1 proteins in the irradiated genome. DNA damage by UVA-lasers was potentiated by either BrdU or Hoechst 33342 pre-sensitization and compared to non-photosensitized cells. DSBs were also induced without BrdU or Hoechst 33342 pre-treatment. Interestingly, no cyclobutane pyrimidine dimers (CPDs) were detected after 405 nm UVA laser micro-irradiation in non-photosensitized cells. The effects of UVA and γ-irradiation were also studied by silver staining of nucleolar organizer regions (AgNORs). This experimental approach revealed changes in the morphology of nucleoli after genome injury. Additionally, to precisely characterize DDR in locally induced DNA lesions, we analysed the kinetics of the 53BP1 protein involved in DDR by fluorescence recovery after photobleaching (FRAP).
- MeSH
- 53BP1 MeSH
- antigeny jaderné MeSH
- buněčné jadérko účinky záření MeSH
- chromozomální proteiny, nehistonové metabolismus MeSH
- DNA vazebné proteiny metabolismus MeSH
- histony metabolismus MeSH
- kinetika MeSH
- luminescentní proteiny metabolismus MeSH
- mikroskopie metody MeSH
- myši MeSH
- poškození DNA * MeSH
- pyrimidinové dimery metabolismus MeSH
- ultrafialové záření * MeSH
- záření gama * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
