Civilization factors are responsible for the increasing of human exposure to mycobacteria from environment, water, and food during the last few decades. Urbanization, lifestyle changes and new technologies in the animal and plant industry are involved in frequent contact of people with mycobacteria. Type 1 diabetes is a multifactorial polygenic disease; its origin is conditioned by the mutual interaction of genetic and other factors. The environmental factors and certain pathogenetic pathways are shared by some immune mediated chronic inflammatory and autoimmune diseases, which are associated with triggers originating mainly from Mycobacterium avium subspecies paratuberculosis, an intestinal pathogen which persists in the environment. Type 1 diabetes and some other chronic inflammatory diseases thus pose the global health problem which could be mitigated by measures aimed to decrease the human exposure to this neglected zoonotic mycobacterium.
An enzyme-linked immunosorbent assay (ELISA) method is described for the semi-quantitative determination of semicarbazide (SEM), the marker residue for the banned nitrofuran drug, nitrofurazone, in chicken eggs. The sample homogenate is subjected to acid hydrolysis and derivatisation with o-nitrobenzaldehyde, followed by ethyl acetate/hexane extraction and detection by ELISA. The ELISA procedure has been validated using 0.3, 1.0 and 3 microg kg(-1) of SEM in fortified samples. Detection capability (CC(ss)) was based on the acceptance of 5% false compliant results for a given concentration level according to Commission Decision 2002/657/EC and was determined to be 0.3 microg kg(-1) with a respective limit of detection of 0.13 microg kg(-1). A validated LC-MS/MS method was used for the analysis of incurred egg samples and the results compared with ELISA. A good correlation between the results obtained from ELISA and LC-MS/MS within the concentration range 0.12-20.3 microg kg(-1) was observed in samples collected from chickens fed with a medicated ration of nitrofurazone (r = 0.992, n = 14). Validated ELISA enabled reliable monitoring of SEM levels in eggs collected from incurred chickens over a 90-day period.
- MeSH
- chromatografie kapalinová metody MeSH
- ELISA metody MeSH
- financování organizované MeSH
- hmotnostní spektrometrie metody MeSH
- karcinogeny analýza MeSH
- kontaminace potravin analýza MeSH
- kontrola léčiv a omamných látek MeSH
- kur domácí MeSH
- nitrofurazon MeSH
- rezidua léčiv analýza MeSH
- semikarbazidy analýza MeSH
- vejce analýza MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- validační studie MeSH
- MeSH
- lidé MeSH
- proteiny chemická syntéza chemie terapeutické užití MeSH
- radioizotopy jodu MeSH
- Check Tag
- lidé MeSH
- Klíčová slova
- pasteurovy vakcíny sněti slezinné,
- MeSH
- Bacillus anthracis imunologie účinky léků MeSH
- bakteriální vakcíny * farmakologie imunologie MeSH
- glukosidy * farmakologie imunologie MeSH
- imunologické techniky MeSH
- koně MeSH
- kultivační techniky metody využití MeSH
- skot MeSH
- vakcíny proti antraxu aplikace a dávkování imunologie terapeutické užití MeSH
- veterinární léky MeSH
- virulence MeSH
- zoonózy MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
