By using dimethyl sulfoxide or Tween 80 (1 or 0.2%), the production of L-lysine was increased by 20-28 and 23-25%, respectively, in regulatory mutant strains of Corynebacterium glutamicum. The stimulation observed is supposed to be caused by influencing cellular surface structures.
Gene manipulation in mycobacteria developed in two phases. In the first phase genes of mycobacteria were transferred into cells of E. coli and Streptomyces lividans. In the second phase, heterologous genes were transferred into mycobacteria either with a shuttle plasmid or hybrid plasmids. A prerequisite for successful gene manipulation in mycobacteria was a thorough understanding of plasmids in mycobacteria. Construction of recombinant DNA molecules contributed not only to the fact that mycobacteria did not remain outside the mainstream of modern genetic research but also to their present practical importance.
- MeSH
- bakteriální geny MeSH
- Escherichia coli genetika MeSH
- Mycobacterium * genetika MeSH
- plazmidy MeSH
- Streptomyces genetika MeSH
- transfekce MeSH
- Publikační typ
- přehledy MeSH
Mutant strains of Mycobacterium sp. V-649 producing highly mucous colonies on a solid cultivation medium were prepared after treatment with N-methyl-N'-nitro-N-nitrosoguanidine and production of the exocellular polysaccharide was tested. The strains were cultivated in media with suitable sugar sources under submerged conditions. It was found that Mycobacterium sp. V-649/15 produces a maximum of 15-19% polymer after a 5-6-d cultivation. Gas chromatography indicated that the exocellular polysaccharide produced by this strain is of glucan type.
